alpha Tubulin 4a Antibody (JM73-24)

Western Blot: alpha Tubulin 4a Antibody (JM73-24) [NBP2-67148] - Analysis of alpha Tubulin 4A on different lysates using anti-alpha Tubulin 4A antibody at 1/1,000 dilution. Positive control: Lane1: A431 Lane2: Rat brain tissue Lane3: NIH-3T3 Lane4: PC-12

Immunocytochemistry/Immunofluorescence: alpha Tubulin 4a Antibody (JM73-24) [NBP2-67148] - Staining alpha Tubulin 4A in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: alpha Tubulin 4a Antibody (JM73-24) [NBP2-67148] - Analysis of paraffin-embedded mouse testes tissue using anti-alpha Tubulin 4A antibody. Counter stained with hematoxylin.

Flow Cytometry: alpha Tubulin 4a Antibody (JM73-24) [NBP2-67148] - Analysis of Hela cells with alpha Tubulin 4A antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Immunocytochemistry/Immunofluorescence: alpha Tubulin 4a Antibody (JM73-24) [NBP2-67148] - Staining alpha Tubulin 4A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: alpha Tubulin 4a Antibody (JM73-24) [NBP2-67148] - Staining alpha Tubulin 4A in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: alpha Tubulin 4a Antibody (JM73-24) [NBP2-67148] - Analysis of paraffin-embedded human placenta tissue using anti-alpha Tubulin 4A antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: alpha Tubulin 4a Antibody (JM73-24) [NBP2-67148] - Analysis of paraffin-embedded human thyroid tissue using anti-alpha Tubulin 4A antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: alpha Tubulin 4a Antibody (JM73-24) [NBP2-67148] - Analysis of paraffin-embedded human tonsil tissue using anti-alpha Tubulin 4A antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: alpha Tubulin 4a Antibody (JM73-24) [NBP2-67148] - Analysis of paraffin-embedded mouse brain tissue using anti-alpha Tubulin 4A antibody. Counter stained with hematoxylin.

Product Discontinued

alpha Tubulin 4a Antibody (JM73-24) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human alpha Tubulin 4a aa 399-448 / 448. (SwissProt: P68366 Human; SwissProt: P68368 Mouse; SwissProt: Q5XIF6 Rat)
• Localization: Cytoskeleton.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TUBA4A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:100-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for alpha Tubulin 4a Antibody (JM73-24)

• alpha 1 (testis specific)
• alpha Tubulin 4a
• Alpha-tubulin 1
• FLJ30169
• H2-ALPHA
• Testis-specific alpha-tubulin
• TUBA1
• TUBA4A
• Tubulin alpha-1 chain
• tubulin alpha-4A chain
• Tubulin H2-alpha
• tubulin, alpha 1
• tubulin, alpha 4a

Background

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintainance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase ads a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle. Alpha tubulin is not suitable as loading control in adipose tissue as expression of tubulin in adipose tissue is very low ( Spiegelman and Farmer, Cell, 1982, 29(1):53-60) in cells undergoing adipose differentiation actin synthesis decreases by 90%. Excellent as a protein loading control antibody.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for alpha Tubulin 4a Antibody (NBP2-67148)(1)

Publication using NBP2-67148

• Fan X, Moustakas I, Torrens-Juaneda V et al. Transcriptional progression during meiotic prophase I reveals sex-specific features and X chromosome dynamics in human fetal female germline PLoS genetics 2021-09-01 [PMID: 34499650] (IHC-P, Human)

Bioinformatics

• Gene Symbol: TUBA4A