alpha-Smooth Muscle Actin Antibody (1A4) [Alexa Fluor® 647]

Human peripheral blood monocytes were stained with Mouse Anti-Human alpha -Smooth Muscle Actin Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # IC1420R, filled histogram) or isotype control antibody (Catalog # IC003R, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: Flow
• Clone: 1A4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.

alpha-Smooth Muscle Actin Antibody (1A4) [Alexa Fluor® 647] Summary

• Immunogen: Peptide corresponding to residues EEEDSTALVC of the N-term of human alpha -smooth muscle actin (1)
• Specificity: Detects human alpha -Smooth Muscle Actin.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACTA2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 0.25-1 ug/10^6 cells
• Application Notes: Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  12 months from date of receipt, 2 to 8 degreesC as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: 0.09% Sodium Azide
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354. This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for alpha-Smooth Muscle Actin Antibody (1A4) [Alexa Fluor® 647]

• AAT6
• ACTA2
• Actin alpha 2
• actin, alpha 2, smooth muscle, aorta
• actin, aortic smooth muscle
• ACTSA
• ACTVS
• alpha 2 actin
• alpha SMA
• alpha smooth muscle actin
• alpha-actin-2
• alpha-SMA
• alphaSmooth Muscle Actin
• alpha-Smooth Muscle Actin
• a-sma
• Cell growth-inhibiting gene 46 protein
• growth-inhibiting gene 46
• MYMY5
• SMA
• smooth muscle actin

Background

alpha -Smooth Muscle Actin has been frequently used as a marker of smooth muscle differentiation (1, 2).

1. Skalli, O. et al. (1986) J. Cell Biol. 103:2787.
2. Oishi, K. et al. (2002) J. Physiol. 540:139.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for alpha-Smooth Muscle Actin Antibody (IC1420R)(5)

Publications using IC1420R

• Park, J;Overbey, EG;Narayanan, SA;Kim, J;Tierney, BT;Damle, N;Najjar, D;Ryon, KA;Proszynski, J;Kleinman, A;Hirschberg, JW;MacKay, M;Afshin, EE;Granstein, R;Gurvitch, J;Hudson, BM;Rininger, A;Mullane, S;Church, SE;Meydan, C;Church, G;Beheshti, A;Mateus, J;Mason, CE; Spatial multi-omics of human skin reveals KRAS and inflammatory responses to spaceflight Nature communications 2024-06-11 [PMID: 38862494] (Immunohistochemistry, Human)
• Devon Duron Ehnes, Ammar Alghadeer, Sesha Hanson-Drury, Yan Ting Zhao, Gwen Tilmes, Julie Mathieu et al. Sci-Seq of Human Fetal Salivary Tissue Introduces Human Transcriptional Paradigms and a Novel Cell Population Frontiers in Dental Medicine 2022-12-21 [PMID: 36540608]
• Hwang WL, Jagadeesh KA, Guo JA Et al. Single-nucleus and spatial transcriptome profiling of pancreatic cancer identifies multicellular dynamics associated with neoadjuvant treatment Nat Genet 2022-07-28 [PMID: 35902743]
  
  Details:
  Citation using the Alexa Fluor 532 version of this antibody.
• Rolando S. Matos, Davide Maselli, John H. McVey, Christian Heiss, Paola Campagnolo 3D Printed Bioreactor Enabling the Pulsatile Culture of Native and Angioplastied Large Arteries Frontiers in Cardiovascular Medicine 2022-06-21 [PMID: 35800166]
• Park J, Foox J, Hether T Et al. System-wide transcriptome damage and tissue identity loss in COVID-19 patients Cell Rep Med 2022-03-02 [PMID: 35233546]
  
  Details:
  Citation using the Alexa Fluor 647 version of this antibody.

FAQs for alpha-Smooth Muscle Actin Antibody (IC1420R). (Showing 1 - 1 of 1 FAQs).

1. I do not see horse listed as a predicted or confirmed species. Would you be able to confirm if any of your alpha-SMA antibodies will react with equine samples?
   • We have not directly tested equine samples nor do we have cited publications that have tested equine samples. Since alpha-Smooth Muscle Actin is a highly conserved sequence, these antibodies should react with most mammal species.

Bioinformatics

• Gene Symbol: ACTA2