alpha-Smooth Muscle Actin Antibody (1A4) [Phycoerythrin]

Human peripheral blood monocytes were stained with Mouse Anti-Human a-Smooth Muscle Actin PE-conjugated Monoclonal Antibody (Catalog # IC1420P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

SMC marker expression after expansion in different media. (a) Histograms of flow cytometric surface marker expression (grey) of CD13, CD14, CD31, CD44, CD45, CD73, CD90, and CD140b on single live cells at passage three or four identified according to the gating strategy shown in Supplementary Fig. S2. Marker expression is given in comparison to the unstained control (white). (b) Gene expression level of SMC marker in isolated cells at passage one or two, normalized to the expression of the housekeeping gene RPL13A. Values of the individual donors are shown as points superimposed onto the boxplots. Expansion medium of each donor is given in brackets; D - DMEM, M - M199, S - supplements (FGF, EGF, insulin, ascorbic acid), VL - VascuLife. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32249802), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: Flow
• Clone: 1A4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

alpha-Smooth Muscle Actin Antibody (1A4) [Phycoerythrin] Summary

• Immunogen: alpha -Smooth Muscle Actin N-terminal Synthetic Decapeptide
• Specificity: Detects human alpha -Smooth Muscle Actin.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACTA2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for alpha-Smooth Muscle Actin Antibody (1A4) [Phycoerythrin]

• AAT6
• ACTA2
• Actin alpha 2
• actin, alpha 2, smooth muscle, aorta
• actin, aortic smooth muscle
• ACTSA
• ACTVS
• alpha 2 actin
• alpha SMA
• alpha smooth muscle actin
• alpha-actin-2
• alpha-SMA
• alphaSmooth Muscle Actin
• alpha-Smooth Muscle Actin
• a-sma
• Cell growth-inhibiting gene 46 protein
• growth-inhibiting gene 46
• MYMY5
• SMA
• smooth muscle actin

Background

alpha ‑Smooth Muscle Actin has been frequently used as a marker of smooth muscle differentiation (1, 2).

1. Skalli, O. et al. (1986) J. Cell Biol. 103:2787.
2. Oishi, K. et al. (2002) J. Physiol. 540:139.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for alpha-Smooth Muscle Actin Antibody (IC1420P)(20)

Publications using IC1420P

• Miquelestorena-Standley, E;da Silva, AVV;Monnier, M;Chadet, S;Piollet, M;Héraud, A;Lemoine, R;Bochaton, T;Derumeaux, G;Roger, S;Ivanes, F;Angoulvant, D; Human peripheral blood mononuclear cells display a temporal evolving inflammatory profile after myocardial infarction and modify myocardial fibroblasts phenotype Scientific reports 2023-10-05 [PMID: 37798364] (ICC, Human)
• Muchli?ska, A;Wenta, R;?ci?ska, W;Markiewicz, A;Suchodolska, G;Senkus, E;?aczek, AJ;Bednarz-Knoll, N; Improved Characterization of Circulating Tumor Cells and Cancer-Associated Fibroblasts in One-Tube Assay in Breast Cancer Patients Using Imaging Flow Cytometry Cancers 2023-08-18 [PMID: 37627197] (Flow Cytometry, Human)
• Anna Piotrowska, Renata Zaucha, Oliwia Król, Michał Aleksander Żmijewski Vitamin D Modulates the Response of Patient-Derived Metastatic Melanoma Cells to Anticancer Drugs International Journal of Molecular Sciences 2023-04-28 [PMID: 37175742] (Immunocytochemistry, Human)
• Anna Piotrowska, Renata Zaucha, Oliwia Król, Michał Aleksander Żmijewski Vitamin D Modulates the Response of Patient-Derived Metastatic Melanoma Cells to Anticancer Drugs International Journal of Molecular Sciences 4/28/2023 [PMID: 37175742]
• Salhab A, Amer J, Lu Y, Safadi R Sodium+/taurocholate cotransporting polypeptide as target therapy for liver fibrosis Gut 7/15/2021 [PMID: 34266968]
• D van Uden, T Koudstaal, JAC van Hulst, IM Bergen, C Gootjes, NW Morrell, G van Loo, JH von der Th, TPP van den Bo, MR Ghigna, F Perros, D Montani, M Kool, KA Boomars, RW Hendriks Central Role of Dendritic Cells in Pulmonary Arterial Hypertension in Human and Mice International Journal of Molecular Sciences, 2021-02-10;22(4):. 2021-02-10 [PMID: 33578743] (IHC, Mouse)
• W Pustlauk, TH Westhoff, L Claeys, T Roch, S Gei beta ler, N Babel Induced osteogenic differentiation of human smooth muscle cells as a model of vascular calcification Sci Rep, 2020-04-06;10(1):5951. 2020-04-06 [PMID: 32249802] (ICC, Human)
• Michal Segal-Salto, Neta Barashi, Avi Katav, Vicktoria Edelshtein, Arnon Aharon, Sharon Hashmueli et al. A blocking monoclonal antibody to CCL24 alleviates liver fibrosis and inflammation in experimental models of liver damage JHEP Reports 2/1/2020 [PMID: 32039405]
• L Swadling, LJ Pallett, MO Diniz, JM Baker, OE Amin, KA Stegmann, AR Burton, NM Schmidt, A Jeffery-Sm, N Zakeri, K Suveizdyte, F Froghi, G Fusai, WM Rosenberg, BR Davidson, A Schurich, AK Simon, MK Maini Human Liver Memory CD8+ T Cells Use Autophagy for Tissue Residence Cell Rep, 2020-01-21;30(3):687-698.e6. 2020-01-21 [PMID: 31968246] (Flow Cytometry, Human)
• Y Yuan, M Li, CH To, TC Lam, P Wang, Y Yu, Q Chen, X Hu, B Ke The Role of the RhoA/ROCK Signaling Pathway in Mechanical Strain-Induced Scleral Myofibroblast Differentiation Invest. Ophthalmol. Vis. Sci., 2018-07-02;59(8):3619-3629. 2018-07-02 [PMID: 30029249] (Flow Cytometry, Guinea Pig)
• J Amer, A Salhab, S Doron, G Morali, R Safadi A novel flow cytometry tool for fibrosis scoring through hepatic stellate cell differentiation Cytometry A, 2018-03-08;0(0):. 2018-03-08 [PMID: 29517852] (Flow Cytometry, Human)
• CY Lo, C Michaeloud, PK Bhavsar, CD Huang, PJ Chang, CH Wang, HP Kuo, KF Chung Reduced suppressive effect of ?2-adrenoceptor agonist on fibrocyte function in severe asthma Respir. Res., 2017-11-21;18(1):194. 2017-11-21 [PMID: 29162108] (Flow Cytometry, Human)
• P Oak, T Pritzke, I Thiel, M Koschlig, DS Mous, A Windhorst, N Jain, O Eickelberg, K Foerster, A Schulze, W Goepel, T Reicherzer, H Ehrhardt, RJ Rottier, P Ahnert, L Gortner, TJ Desai, A Hilgendorf Attenuated PDGF signaling drives alveolar and microvascular defects in neonatal chronic lung�disease EMBO Mol Med, 2017-11-01;0(0):. 2017-11-01 [PMID: 28923828] (Flow Cytometry, Mouse)
• Kwan P, Ding J, Tredget E MicroRNA 181b regulates decorin production by dermal fibroblasts and may be a potential therapy for hypertrophic scar. PLoS ONE, 2015-04-02;10(4):e0123054. 2015-04-02 [PMID: 25837671] (Flow Cytometry, Human)
• Huang L, Nakayama H, Klagsbrun M, Mulliken J, Bischoff J Glucose transporter 1-positive endothelial cells in infantile hemangioma exhibit features of facultative stem cells. Stem Cells, 2015-01-01;33(1):133-45. 2015-01-01 [PMID: 25187207] (Flow Cytometry, Human)
• Abu-Tair L, Axelrod J, Doron S, Ovadya Y, Krizhanovsky V, Galun E, Amer J, Safadi R Natural killer cell-dependent anti-fibrotic pathway in liver injury via Toll-like receptor-9. PLoS ONE, 2013-12-10;8(12):e82571. 2013-12-10 [PMID: 24340043] (Flow Cytometry, Human)
• Dendooven A, van Oostrom O, van der Giezen DM, Leeuwis JW, Snijckers C, Joles JA, Robertson EJ, Verhaar MC, Nguyen TQ, Goldschmeding R Loss of endogenous bone morphogenetic protein-6 aggravates renal fibrosis. Am. J. Pathol., 2011-03-01;178(3):1069-79. 2011-03-01 [PMID: 21356359] (Flow Cytometry, Human)
• Semenov O, Koestenbauer S, Riegel M, Zech N, Zimmermann R, Zisch A, Malek A Multipotent mesenchymal stem cells from human placenta: critical parameters for isolation and maintenance of stemness after isolation. Am J Obstet Gynecol, 2009-12-24;202(2):193.e1-193.e1. 2009-12-24 [PMID: 20035913] (Flow Cytometry, Human)
• Asosingh K, Aldred MA, Vasanji A, Drazba J, Sharp J, Farver C, Comhair SA, Xu W, Licina L, Huang L, Anand-Apte B, Yoder MC, Tuder RM, Erzurum SC Circulating angiogenic precursors in idiopathic pulmonary arterial hypertension. Am. J. Pathol., 2008-02-07;172(3):615-27. 2008-02-07 [PMID: 18258847] (Flow Cytometry, Human)
• Nazareth MR, Broderick L, Simpson-Abelson MR, Kelleher RJ, Yokota SJ, Bankert RB Characterization of human lung tumor-associated fibroblasts and their ability to modulate the activation of tumor-associated T cells. J. Immunol., 2007-05-01;178(9):5552-62. 2007-05-01 [PMID: 17442937] (Flow Cytometry, Human)

FAQs for alpha-Smooth Muscle Actin Antibody (IC1420P). (Showing 1 - 1 of 1 FAQs).

1. I do not see horse listed as a predicted or confirmed species. Would you be able to confirm if any of your alpha-SMA antibodies will react with equine samples?
   • We have not directly tested equine samples nor do we have cited publications that have tested equine samples. Since alpha-Smooth Muscle Actin is a highly conserved sequence, these antibodies should react with most mammal species.

Bioinformatics

• Gene Symbol: ACTA2