alpha-Smooth Muscle Actin Antibody (1A4) [Allophycocyanin]

Human peripheral blood monocytes were stained with Mouse Anti-Human a-Smooth Muscle Actin APC-conjugated Monoclonal Antibody (Catalog # IC1420A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

FGFR1 and TGF beta signaling activity in smooth muscle cells in a mouse atherosclerosis modelADissected mouse aorta demonstrating lipid‐rich plaques in brachiocephalic artery after 4 months of high‐fat diet compared to the normal diet in Apoe−/− mice. Panels b, d are cross sections of brachiocephalic artery from a, c stained with Oil Red O. L: lumen. Scale bar: 4 mm. n = 3 mice per group.BHistological analysis of mouse normal artery or atherosclerotic plaque in brachiocephalic artery with anti‐SM alpha ‐actin, anti‐Notch3, and anti‐SM‐MHC antibodies. Nuclei were counterstained with DAPI (blue). L: lumen. Scale bar: 62 μm. n = 3 mice per group.C–FAnalysis of brachiocephalic artery of Apoe−/− mice maintained for 4 months on either normal or high‐fat diet using anti‐CD31 (green), anti‐p‐FGFR1 (red; C), anti‐FGFR1 (red; D), anti‐p‐Smad2 (red; E), and anti‐p‐Smad3 (red; F) antibodies. Nuclei were counterstained with DAPI (blue). Scale bar: 62 μm. L: lumen. M: media. n = 6 mice per group.G–JQuantification of the number of medial smooth muscle cells expressing p‐FGFR1, FGFR1, p‐Smad2, and p‐Smad3. ND: normal diet. HFD: high‐fat diet.Data information: All data shown as means ± SD (***P < 0.001 compared to ND, NS: not significant compared to ND; unpaired two‐tailed Student's t‐test). A full table of P‐values for this figure is shown in Appendix Table S1. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/27189169), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: Flow
• Clone: 1A4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

alpha-Smooth Muscle Actin Antibody (1A4) [Allophycocyanin] Summary

• Immunogen: alpha -Smooth Muscle Actin N-terminal Synthetic Decapeptide
• Specificity: Detects human alpha -Smooth Muscle Actin.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACTA2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for alpha-Smooth Muscle Actin Antibody (1A4) [Allophycocyanin]

• AAT6
• ACTA2
• Actin alpha 2
• actin, alpha 2, smooth muscle, aorta
• actin, aortic smooth muscle
• ACTSA
• ACTVS
• alpha 2 actin
• alpha SMA
• alpha smooth muscle actin
• alpha-actin-2
• alpha-SMA
• alphaSmooth Muscle Actin
• alpha-Smooth Muscle Actin
• a-sma
• Cell growth-inhibiting gene 46 protein
• growth-inhibiting gene 46
• MYMY5
• SMA
• smooth muscle actin

Background

alpha ‑Smooth Muscle Actin has been frequently used as a marker of smooth muscle differentiation (1, 2).

1. Skalli, O. et al. (1986) J. Cell Biol. 103:2787.
2. Oishi, K. et al. (2002) J. Physiol. 540:139.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for alpha-Smooth Muscle Actin Antibody (IC1420A)(14)

Publications using IC1420A

• Yonemura, A;Semba, T;Zhang, J;Fan, Y;Yasuda-Yoshihara, N;Wang, H;Uchihara, T;Yasuda, T;Nishimura, A;Fu, L;Hu, X;Wei, F;Kitamura, F;Akiyama, T;Yamashita, K;Eto, K;Iwagami, S;Iwatsuki, M;Miyamoto, Y;Matsusaki, K;Yamasaki, J;Nagano, O;Saya, H;Song, S;Tan, P;Baba, H;Ajani, JA;Ishimoto, T; Mesothelial cells with mesenchymal features enhance peritoneal dissemination by forming a protumorigenic microenvironment Cell reports 2023-12-30 [PMID: 38232734] (Flow Cytometry, Mouse)
• Hye Jeong, J;Park, S;Lee, S;Kim, Y;Kyong Shim, I;Jeong, SY;Kyung Choi, E;Kim, J;Jun, E; Orthotopic model of pancreatic cancer using CD34+ humanized mice and generation of tumor organoids from humanized tumors International immunopharmacology 2023-06-16 [PMID: 37331294] (Flow Cytometry, Mouse)
• D Karakas, M Erkisa, RO Akar, G Akman, EY Senol, E Ulukaya Targeting Periostin Expression Makes Pancreatic Cancer Spheroids More Vulnerable to Natural Killer Cells Biomedicines, 2023-01-19;11(2):. 2023-01-19 [PMID: 36830807] (Flow Cytometry, Human)
• Malacrida B, Nichols S, Maniati E et al. A human multi-cellular model shows how platelets drive production of diseased extracellular matrix and tissue invasion iScience 6/25/2021 [PMID: 34189439]
• Delaine-Smith R, Maniati E, Malacrida B et al. Modelling TGF beta R and Hh pathway regulation of prognostic matrisome molecules in ovarian cancer iScience 5/1/2021 [PMID: 34189438]
• A Kasprzyk-P, A Wojciechow, M Kuc, J Zielinski, A Parulski, M Kusmierczy, A Lutynska, K Kozar-Kami microRNA expression profile in Smooth Muscle Cells isolated from thoracic aortic aneurysm samples Adv Med Sci, 2019-04-22;64(2):331-337. 2019-04-22 [PMID: 31022558] (Flow Cytometry, Human)
• S Nürnberger, C Lindner, J Maier, K Strohmeier, C Wurzer, P Slezak, S Suessner, W Holnthoner, H Redl, S Wolbank, E Priglinger Adipose-tissue-derived therapeutic cells in their natural environment as an autologous cell therapy strategy: the microtissue-stromal vascular fraction Eur Cell Mater, 2019-02-22;37(0):113-133. 2019-02-22 [PMID: 30793275] (Flow Cytometry, Human)
• S Shichino, S Ueha, S Hashimoto, M Otsuji, J Abe, T Tsukui, S Deshimaru, T Nakajima, M Kosugi-Kan, FH Shand, Y Inagaki, H Shimano, K Matsushima Transcriptome network analysis identifies protective role of the LXR/SREBP-1c axis in murine pulmonary fibrosis JCI Insight, 2019-01-10;4(1):. 2019-01-10 [PMID: 30626759] (Flow Cytometry, Mouse)
• K Souma, S Shichino, S Hashimoto, S Ueha, T Tsukui, T Nakajima, HI Suzuki, FHW Shand, Y Inagaki, T Nagase, K Matsushima Lung fibroblasts express a miR-19a-19b-20a sub-cluster to suppress TGF-?-associated fibroblast activation in murine pulmonary fibrosis Sci Rep, 2018-11-09;8(1):16642. 2018-11-09 [PMID: 30413725] (Flow Cytometry, Mouse)
• A Costa, Y Kieffer, A Scholer-Da, F Pelon, B Bourachot, M Cardon, P Sirven, I Magagna, L Fuhrmann, C Bernard, C Bonneau, M Kondratova, I Kuperstein, A Zinovyev, AM Givel, MC Parrini, V Soumelis, A Vincent-Sa, F Mechta-Gri Fibroblast Heterogeneity and Immunosuppressive Environment in Human Breast Cancer Cancer Cell, 2018-02-15;0(0):. 2018-02-15 [PMID: 29455927] (FACS, Human)
• C Mishima, N Kagara, JI Ikeda, E Morii, T Miyake, T Tanei, Y Naoi, M Shimoda, K Shimazu, SJ Kim, S Noguchi Mutational analysis of AKT1 and PIK3CA in intraductal papillomas of the breast with special reference to cellular components Am. J. Pathol., 2018-02-16;0(0):. 2018-02-16 [PMID: 29454754] (Flow Cytometry, Human)
• E Amati, O Perbellini, G Rotta, M Bernardi, K Chieregato, S Sella, F Rodeghiero, M Ruggeri, G Astori High-throughput immunophenotypic characterization of bone marrow- and cord blood-derived mesenchymal stromal cells reveals common and differentially expressed markers: identification of angiotensin-converting enzyme (CD143) as a marker differentially expressed between adult and perinatal tissue sources Stem Cell Res Ther, 2018-01-16;9(1):10. 2018-01-16 [PMID: 29338788] (Flow Cytometry, Human)
• Guangxin Li, Lingfeng Qin, Lei Wang, Xuan Li, Alexander W. Caulk, Jian Zhang et al. Inhibition of the mTOR pathway in abdominal aortic aneurysm: implications of smooth muscle cell contractile phenotype, inflammation, and aneurysm expansion American Journal of Physiology-Heart and Circulatory Physiology 6/1/2017 [PMID: 28213405]
• Chen PY, Qin L, Baeyens N et al. Endothelial-to-mesenchymal transition drives atherosclerosis progression. J Clin Invest 10/26/2015 [PMID: 26517696]

FAQs for alpha-Smooth Muscle Actin Antibody (IC1420A). (Showing 1 - 1 of 1 FAQs).

1. I do not see horse listed as a predicted or confirmed species. Would you be able to confirm if any of your alpha-SMA antibodies will react with equine samples?
   • We have not directly tested equine samples nor do we have cited publications that have tested equine samples. Since alpha-Smooth Muscle Actin is a highly conserved sequence, these antibodies should react with most mammal species.

Bioinformatics

• Gene Symbol: ACTA2