alpha-Smooth Muscle Actin Antibody (1A4) [PerCP (Peridinin-chlorophyll Protein Complex)]

Human peripheral blood monocytes were stained with Mouse Anti-Human a-Smooth Muscle Actin PerCP-conjugated Monoclonal Antibody (Catalog # IC1420C, filled histogram) or isotype control antibody (Catalog # IC003C, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: Flow
• Clone: 1A4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: PerCP (Peridinin-chlorophyll Protein Complex)

alpha-Smooth Muscle Actin Antibody (1A4) [PerCP (Peridinin-chlorophyll Protein Complex)] Summary

• Immunogen: alpha -Smooth Muscle Actin N-terminal Synthetic Decapeptide
• Specificity: Detects human alpha -Smooth Muscle Actin.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACTA2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for alpha-Smooth Muscle Actin Antibody (1A4) [PerCP (Peridinin-chlorophyll Protein Complex)]

• AAT6
• ACTA2
• Actin alpha 2
• actin, alpha 2, smooth muscle, aorta
• actin, aortic smooth muscle
• ACTSA
• ACTVS
• alpha 2 actin
• alpha SMA
• alpha smooth muscle actin
• alpha-actin-2
• alpha-SMA
• alphaSmooth Muscle Actin
• alpha-Smooth Muscle Actin
• a-sma
• Cell growth-inhibiting gene 46 protein
• growth-inhibiting gene 46
• MYMY5
• SMA
• smooth muscle actin

Background

alpha ‑Smooth Muscle Actin has been frequently used as a marker of smooth muscle differentiation (1, 2).

1. Skalli, O. et al. (1986) J. Cell Biol. 103:2787.
2. Oishi, K. et al. (2002) J. Physiol. 540:139.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for alpha-Smooth Muscle Actin Antibody (IC1420C)(1)

Publication using IC1420C

• Jun-Hung Cho, Bhaumik Patel, Santosh Bonala, Sasikanth Manne, Yan Zhou, Surya K Vadrevu, Jalpa Patel, Marco Peronaci, Shanawaz Ghouse, Elizabeth P Henske, Fabrice Roegiers, Krinio Giannikou, David J Kwiatkows, Hossein Mansouri, Maciej M Markiewsk, Brandon White, Magdalena Karbownic Notch transactivates Rheb to maintain the multipotency of TSC-null cells. Nature Communications, 2017-11-29;0(0):2041-1723. 2017-11-29 [PMID: 29184052] (Flow Cytometry, Rat, Human)

FAQs for alpha-Smooth Muscle Actin Antibody (IC1420C). (Showing 1 - 1 of 1 FAQ).

1. I do not see horse listed as a predicted or confirmed species. Would you be able to confirm if any of your alpha-SMA antibodies will react with equine samples?
   • We have not directly tested equine samples nor do we have cited publications that have tested equine samples. Since alpha-Smooth Muscle Actin is a highly conserved sequence, these antibodies should react with most mammal species.

Bioinformatics

• Gene Symbol: ACTA2