alpha-Internexin Antibody - BSA Free

Western Blot: alpha-Internexin Antibody [NB110-93355] - Western blot of rat cortex lysate showing specific immunolabeling of the ~ 66k alpha internexin protein.

Immunocytochemistry/Immunofluorescence: alpha-Internexin Antibody [NB110-93355] - Immunofluorescence of a section of rat cerebellum showing labeling of axons of granule cell and dendrites of Purkinje with alpha-internexin, labeling of the myelin sheaths around the axons with MBP, and additional nuclear staining with DAPI (blue).

Immunocytochemistry/Immunofluorescence: alpha-Internexin Antibody [NB110-93355] - Immunostaining of cultured neurons and glia showing specific labeling of neuronal processes (red) using our alpha-internexin antibody and microglia (green) with a coronin 1a antibody.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Chicken
• Conjugate: Unconjugated
• Format: BSA Free

alpha-Internexin Antibody - BSA Free Summary

• Description: Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).
• Immunogen: Recombinant rat alpha-internexin expressed in and purified from E. coli. Accession # P23565
• Marker: Immature Neuronal Marker
• Specificity: Specific for endogenous levels of the ~66 kDa alpha-Internexin protein. Minor bands at ~150 kDa are probably covalent dimers and bands at ~50 kDa represent alpha-internexin breakdown products.
• Isotype: IgY
• Clonality: Polyclonal
• Host: Chicken
• Gene: INA
• Purity: IgY purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:500
  • Immunohistochemistry 1:1000 - 1:5000
  • Western Blot 1:5000
• Theoretical MW: 66 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Reactivity assumed based on 100% sequence homology to a wide variety of mammalian species.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 10mM Sodium Azide
• Purity: IgY purified

Notes

Chicken products cannot be exported to Canada.

Alternate Names for alpha-Internexin Antibody - BSA Free

• 66 kDa neurofilament protein
• alphaInternexin
• alpha-Internexin
• alpha-Inx
• FLJ18662
• FLJ57501
• INA
• internexin neuronal intermediate filament protein, alpha
• MGC12702
• NEF5
• neurofilament 5 (66kD)
• Neurofilament 5
• Neurofilament-66
• neurofilament-66, tax-binding protein
• NF-66
• NF-66alpha-internexin
• TXBP-1

Background

Alpha-internexin is a Class IV intermediate filament originally discovered as it co-purifies with other neurofilament subunits (1). Alpha-internexin is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. It is expressed only in neurons and in large amounts early in neuronal development, but is down-regulated in many neurons as development proceeds. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to alpha-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition, recent studies show a marked up-regulation of alpha-internexin during neuronal regeneration (2). The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest. Recently Cairns et al. used this antibody to show that alpha-internexin is an abundant component of the inclusions of neurofilament inclusion body disease (NFID), a serious human neurodegenerative disorder (3,4). The antibody was also used to confirm the presence of circulating auto-antibodies to alpha-internexin in the sera of some patients with endocrine autoimmunity, as well as in some normal individuals (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: INA
• Entrez:
  • 9118(Human)
  • 226180(Mouse)
  • 24503(Rat)
• Uniprot:
  • P23565(Rat)