alpha-Internexin Antibody (529724) [Unconjugated]

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Western blot shows lysates of human cortex tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti‑Human alpha -Internexin Monoclonal Antibody (Catalog # MAB4844) followed by HRP-conjugated Anti-Mouse IgG ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
529724
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

alpha-Internexin Antibody (529724) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human alpha -Internexin
Val230-Glu450
Accession # Q16352
Specificity
Detects human  alpha ‑Internexin in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) NF-M or rhVimentin is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
INA
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for alpha-Internexin Antibody (529724) [Unconjugated]

  • 66 kDa neurofilament protein
  • alphaInternexin
  • alpha-Internexin
  • alpha-Inx
  • FLJ18662
  • FLJ57501
  • INA
  • internexin neuronal intermediate filament protein, alpha
  • MGC12702
  • NEF5
  • neurofilament 5 (66kD)
  • Neurofilament 5
  • Neurofilament-66
  • neurofilament-66, tax-binding protein
  • NF-66
  • NF-66alpha-internexin
  • TXBP-1

Background

alpha -Internexin ( alpha -Inx; also NF-66) is a 66 kDa member of the intermediate filament (IF) protein family. It was the first of two molecules named for its presumed interaction with cytoskeletal proteins. alpha -Inx is one of four Class IV neuronal IF proteins. It both self-assembles and complexes with NF-L, H and M in select cortical and cerebellar neurons. alpha -Inx contains one DNA-binding region (aa 10‑92), a poly‑Glu segment (aa 449‑454) and three serine phosphorylation sites (Ser72/335/496). There is one 494 aa alternative splice form that shows multiple short sequence aa substitutions in the first 200 amino acids. Over aa 230‑450, human alpha -Inx shows more that 96% aa identity with mouse, canine and rat alpha -Inx.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol INA
Uniprot