alpha-Galactosidase A/GLA Antibody - BSA Free

Western Blot: alpha-Galactosidase A/GLA Antibody [NBP2-16576] - Whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Galactosidase alpha antibody at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: alpha-Galactosidase A/GLA Antibody [NBP2-16576] - Analysis of paraffin-embedded Cal27 xenograft, using antibody at 1:100 dilution.

Western Blot: alpha-Galactosidase A/GLA Antibody [NBP2-16576] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE gel, diluted at 1:1000.

Western Blot: alpha-Galactosidase A/GLA Antibody [NBP2-16576] - Rat tissue extracts (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Galactosidase alpha antibody [N1C2] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunoprecipitation: alpha-Galactosidase A/GLA Antibody [NBP2-16576] - Immunoprecipitation of GLA protein from 293T whole cell extracts using 5 ug of Galactosidase alpha antibody [N1C2]. Western blot analysis was performed using Galactosidase alpha antibody [N1C2]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

• Reactivity: Hu, Rt
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

alpha-Galactosidase A/GLA Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human alpha-Galactosidase A/GLA. The exact sequence is proprietary.
• Localization: Lysosome
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GLA
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 49 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Pig (84%), Bovine (80%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for alpha-Galactosidase A/GLA Antibody - BSA Free

• agalsidase alfa
• Agalsidase alpha
• Agalsidase
• Alpha-D-galactosidase A
• alpha-D-galactoside galactohydrolase 1
• Alpha-D-galactoside galactohydrolase
• alpha-gal A
• alpha-galactosidase A
• EC 3.2.1
• EC 3.2.1.22
• GALA
• galactosidase, alpha
• GLA
• Melibiase

Background

This gene encodes a homodimeric glycoprotein that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. This enzyme predominantly hydrolyzes ceramide trihexoside, and it can catalyze the hydrolysis of melibiose into galactose and glucose. A variety of mutations in this gene affect the synthesis, processing, and stability of this enzyme, which causes Fabry disease, a rare lysosomal storage disorder that results from a failure to catabolize alpha-D-galactosyl glycolipid moieties. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GLA
• Uniprot:
  • P06280()