alpha-Fetoprotein/AFP Antibody (SR2120) - BSA Free

Western Blot: alpha-Fetoprotein/AFP Antibody (SR2120) [NBP3-21716] - Western blot analysis using NBP3-21716 in HepG2 cell lysate.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clone: SR2120
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

alpha-Fetoprotein/AFP Antibody (SR2120) - BSA Free Summary

• Description: Novus Biologicals Rabbit alpha-Fetoprotein/AFP Antibody (SR2120) - BSA Free (NBP3-21716) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human alpha-Fetoprotein/AFP (Uniprot #: P02771)
• Specificity: Detects endogenous levels of total alpha-Fetoprotein/AFP
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: AFP
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunoprecipitation 1:50
  • Western Blot 1:500-1:2000
• Theoretical MW: 68 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for alpha-Fetoprotein/AFP Antibody (SR2120) - BSA Free

• AFP
• Alpha-1-fetoprotein
• alpha-fetoglobulin
• alpha-Fetoprotein
• DSCAM2
• FETA
• HP
• HPAFP

Background

Alpha 1 Fetoprotein encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AFP