alpha-Fetoprotein/AFP: Novus Biologicals

MAB1368

alpha-Fetoprotein/AFP Antibody (189502) [Unconjugated]

(3 Reviews)

100 μg

MAB1368

HAF018). A specific band was detected for a-Fetoprotein/AFP at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse a-Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF018). A specific band was detected for a-Fetoprotein/AFP at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse a-Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a-Fetoprotein/AFP at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

a-Fetoprotein/AFP was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human/Mouse a-Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_lightbox">

HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human/Mouse a-Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368, filled histogram) or isotype control antibody (Catalog # <a class=

MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_thumb" />

Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for alpha ‑Fetoprotein/AFP at approximately 70 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human/Mouse alpha ‑Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

HAF018). A specific band was detected for a-Fetoprotein/AFP at approximately 70 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

<b>Genetic Strategies Validation. </b>Western blot shows lysates of HepG2 human hepatocellular carcinoma parental cell line and a-Fetoprotein/AFP knockout HepG2 cell line (KO). PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse a-Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF018). A specific band was detected for a-Fetoprotein/AFP at approximately 70 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

MAB002, open histogram) followed by PE-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0102B). No staining in the a-Fetoprotein/AFP knockout HepG2 cell line was observed. View our protocol for Staining Membrane-associated Proteins." class="big_lightbox">

<b>Genetic Strategies Validation. </b>a-Fetoprotein/AFP knockout HepG2 hepatocellular carcinoma cell line was stained with Mouse Anti-Human a-Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368, filled histogram) or isotype control antibody (Catalog # <a class=

MAB002, open histogram) followed by PE-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0102B). No staining in the a-Fetoprotein/AFP knockout HepG2 cell line was observed. View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

Functional glycosylation of alpha ‐dystroglycan and characterization of dystroglycanopathy patient‐specific iPSCsCurrent model of the core M3 functional glycan structure on alpha ‐dystroglycan and enzymes involved in its synthesis. ECM ligands, such as laminins, bind to the Xyl‐GlucA disaccharide repeats (IIH6 epitope). Man, mannose; GlcNAc, N‐acetylglucosamine; GalNAc, N‐acetylgalactosamine; Rbo5P, ribitol‐5‐phosphate; Xyl, xylose; GlcA, glucuronic acid.Representative images of immunostaining demonstrate that FKRP‐iPSCs express specific pluripotency‐associated markers, including NANOG, OCT4, Tra‐1‐60, and SSEA4.FKRP‐iPSCs have a normal karyotype.In vitro differentiation of FKRP‐iPSCs to cells representing ectoderm ( beta ‐III Tubulin, Tuj1), mesoderm (SMA, smooth muscle actin), and endoderm (AFP, alpha ‐fetoprotein).Data information: Scale bars, 50 μm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31566294), licensed under a CC-BY license. Not internally tested by R&D Systems.

Verification of the pluripotency of the iPSC lines with the TDP-43 A90V mutation.(A) Fluorescence microscopy images of the expression of the pluripotency markers NANOG, OCT4, SSEA4, TRA-1-60, and TRA-1-81 in control (37L25) and patient (36L10) iPSC lines. Scale bar: 20 µm. (B) All iPSC lines differentiated into cells of the three germ layers, as shown by expression of desmin (mesoderm), TUJ1 (ectoderm), and alpha-fetoprotein (AFP, endoderm). These analyses indicate iPSC lines generated here are indeed pluripotent. Scale bar: 20 µm. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0076055), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>Unchanged tumor marker levels in liver, lung, brain, and skin of plasma-treated mice. Representative images of immunohistochemistry of different tumor markers (TM): AFP (I) in liver, beta 2M in brain (II), CEA in lung (III), as well as NSE staining in skin tissue (IV) after one year in positive control (+ve ctrl, left) and plasma-treated (right) animals (A). Scale bar 50 µm (right columns) or 100 µm (left columns). Using ELISA, we analyzed AFP (I), and calcitonin (CT) (II), a TM of medullary thyroid carcinoma, in blood serum (B; * p < 0.05; (n > 9). The mRNA expression levels of five TM and beta -actin in liver, brain, lung, and ear skin tissues from plasma- and untreated (ctrl) mice were compared with organs from a HCC-bearing mouse (+ve ctrl). At least three independent experiments were performed and summarized in the indicated experimental groups (m, male; f, female). AFP, alpha -fetoprotein; NOPE, neighbor of Punc 11; beta 2M, beta 2-microglobulin; NSE, neuron specific enolase; CEA, carcinoembryonic antigen (C). Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/18/4/868), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>Pertussis toxin does not affect the maintenance of pluripotency pluripotent stem cell colonies or the ability to form all three embryonic germ layers.(A) Pertussis toxin does not affect the maintenance of pluripotency of hES or hiPS cells as assessed by the expression of the pluripotency markers Nanog, Oct4, Sox2, TDGF1, and ZFP42 by quantitative real-time PCR. (B) Immunocytochemistry of TRA-1-81 (green) and Oct-3/4 (red) also did not reveal differences between hES or hiPS cells treated with pertussis toxin and control treatments in pluripotent stem cell colonies. Nuclei were stained with Hoechst (blue). Scale bar, 200 µm. (C) hES or iPS cells treated with pertussis toxin formed embryoid bodies and differentiated into cell types of the all three embryonic germ layers as assessed by immunocytochemistry for alpha -fetoprotein (a marker of endoderm), alpha -smooth muscle actin (mesoderm) and beta III-tubulin (ectoderm). Scale bar, 100 µm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/19936228), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	IC1368U-100UG
Alexa Fluor 405	IC1368V-100UG
Alexa Fluor 488	IC1368G
Alexa Fluor 532	FAB1368X
Alexa Fluor 594	IC1368T-100UG
Alexa Fluor 647	IC1368R-100UG
Alexa Fluor 700	IC1368N-100UG
Alexa Fluor 750	IC1368S-100UG
Alexa Fluor Plus 405	FAB1368AFP405-100UG
Alexa Fluor Plus 488	FAB1368AFP488-100UG
Alexa Fluor Plus 555	FAB1368AFP555-100UG
Alexa Fluor Plus 594	FAB1368AFP594-100UG
Alexa Fluor Plus 647	FAB1368AFP647-100UG
Alexa Fluor Plus 680	FAB1368AFP680-100UG
Allophycocyanin	FAB1368A
Biotin	FAB1368B
CoraFluor 1	FAB1368CL1
DyLight 350	FAB1368D
DyLight 405	FAB1368E
DyLight 488	FAB1368K
DyLight 550	FAB1368L
DyLight 594	FAB1368M
DyLight 650	FAB1368W
DyLight 680	FAB1368Y
DyLight 755	FAB1368Z
FITC	FAB1368F
HRP	FAB1368H
Janelia Fluor 525	FAB1368JF525
Janelia Fluor 549	FAB1368I
Janelia Fluor 646	FAB1368J
Janelia Fluor 669	FAB1368JF669
PerCP	FAB1368C
Phycoerythrin	IC1368P
mFluor Violet 450 SE	FAB1368MFV450
mFluor Violet 500 SE	FAB1368MFV500
mFluor Violet 610 SE	FAB1368MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	IC1368G
Carrier Free	IC1368P
Carrier Free	MAB1368-SP
Carrier Free	IC1368V-100UG
Carrier Free	IC1368U-100UG
Carrier Free	IC1368T-100UG
Carrier Free	IC1368S-100UG
Carrier Free	IC1368R-100UG
Carrier Free	IC1368N-100UG

compare

(select up to 3 products from this page to compare)

MAB1369

alpha-Fetoprotein/AFP Antibody (189506) [Unconjugated]

(21 Publications)

100 μg

MAB1369

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

a-Fetoprotein/AFP was detected in immersion fixed BG01V human embryonic stem cells differentiated to hepatocytes using Mouse Anti-Human a-Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="a-Fetoprotein/AFP was detected in immersion fixed BG01V human embryonic stem cells differentiated to hepatocytes using Mouse Anti-Human a-Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

MAB004, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_lightbox">

HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human a-Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1369, filled histogram) or isotype control antibody (Catalog # <a class=

MAB004, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

<b>Genetic Strategies Validation. </b>a-Fetoprotein/AFP was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line but is not detected in a-Fetoprotein/AFP knockout (KO) HepG2 Human Cell Line cell line using Mouse Anti-Human a-Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	IC1369U-100UG
Alexa Fluor 405	IC1369V-100UG
Alexa Fluor 488	IC1369G-100UG
Alexa Fluor 532	FAB1369X
Alexa Fluor 594	IC1369T-100UG
Alexa Fluor 647	IC1369R-100UG
Alexa Fluor 700	IC1369N-100UG
Alexa Fluor 750	IC1369S-100UG
Alexa Fluor Plus 405	FAB1369AFP405-100UG
Alexa Fluor Plus 488	FAB1369AFP488-100UG
Alexa Fluor Plus 555	FAB1369AFP555-100UG
Alexa Fluor Plus 594	FAB1369AFP594-100UG
Alexa Fluor Plus 647	FAB1369AFP647-100UG
Alexa Fluor Plus 680	FAB1369AFP680-100UG
Allophycocyanin	FAB1369A
Biotin	FAB1369B
CoraFluor 1	FAB1369CL1
DyLight 350	FAB1369D
DyLight 405	FAB1369E
DyLight 488	FAB1369K
DyLight 550	FAB1369L
DyLight 594	FAB1369M
DyLight 650	FAB1369W
DyLight 680	FAB1369Y
DyLight 755	FAB1369Z
FITC	FAB1369F
HRP	FAB1369H
Janelia Fluor 525	FAB1369JF525
Janelia Fluor 549	FAB1369I
Janelia Fluor 646	FAB1369J
Janelia Fluor 669	FAB1369JF669
PE	FAB1369P
PerCP	FAB1369C
mFluor Violet 450 SE	FAB1369MFV450
mFluor Violet 500 SE	FAB1369MFV500
mFluor Violet 610 SE	FAB1369MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB1369-SP
Carrier Free	IC1369V-100UG
Carrier Free	IC1369U-100UG
Carrier Free	IC1369T-100UG
Carrier Free	IC1369S-100UG
Carrier Free	IC1369R-100UG
Carrier Free	IC1369N-100UG
Carrier Free	IC1369G-100UG

compare

(select up to 3 products from this page to compare)

AF5369

alpha-Fetoprotein/AFP Antibody

(1 Review)

(12 Publications)

100 μg

AF5369

HAF019). A specific band was detected for a-Fetoprotein/AFP at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_lightbox">

Western blot shows lysates of mouse plasma and rat placenta tissue. PVDF membrane was probed with 1 µg/mL of Mouse a-Fetoprotein/AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5369) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF019). A specific band was detected for a-Fetoprotein/AFP at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." alt="Western blot shows lysates of mouse plasma and rat placenta tissue. PVDF membrane was probed with 1 µg/mL of Mouse a-Fetoprotein/AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5369) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for a-Fetoprotein/AFP at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_thumb" />

Extensive hepatocyte damage by hepatocyte-specific activation of FOXO3 associated with upregulation of HCC-concomitant factors. a More inflammation can be observed in livers from 9-week-old FOXO3CAHep transgenic mice than in control animals. b Expression of Trp53 and Myc by qPCR in livers from 9-week-old control and FOXO3CAHep transgenic mice. c Western blot analysis in livers from 9-week-old control and FOXO3CAHep mice with p53 and c-Myc and GAPDH as loading control (left panel), as well as densitometric quantification (middle and right panels). d Expression of Afp by qPCR in livers from 9-week-old control and FOXO3CAHep transgenic mice (n = 4). e Representative IHC staining for AFP (bar = 100 μm).f Western blot analysis in livers from 9-week-old control and FOXO3CAHep mice with AFP and GAPDH as loading control (upper panel), as well as densitometric quantification (lower panel). g Serum AFP levels in 9-week-old control and FOXO3CAHep mice Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31488102), licensed under a CC-BY license. Not internally tested by R&D Systems.

Enhanced proliferation and elevated expression of AFP and Vimentin in livers of DEN-injected 40-week-old FOXO3CAHep transgenic mice. a Representative IHC staining for Ki67, AFP, p53, E-Cadherin, N-Cadherin, and Vimentin in 40-week-old DEN-injected control and DEN-injected FOXO3CAHep transgenic mice (bar = 100 μm). b Scoring of Ki67-positivity (n = 4). c Western blot analysis in livers from 40-week-old DEN-injected control and DEN-injected FOXO3CAHep transgenic mice with AFP, c-Myc, p53, E-Cadherin, N-Cadherin, Vimentin and GAPDH as loading control (upper part), as well as densitometric quantification (lower part) Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31488102), licensed under a CC-BY license. Not internally tested by R&D Systems.

MAB5369) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse ?‑Fetoprotein/AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5369) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Mouse/Rat IGF-I was serially diluted and captured by Rat Anti-Mouse ?‑Fetoprotein/AFP Monoclonal Antibody (Catalog # <a class=

MAB5369) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse ?‑Fetoprotein/AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5369) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

MAB5369) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse alpha ‑Fetoprotein/AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5369) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Mouse/Rat IGF-I was serially diluted and captured by Rat Anti-Mouse alpha ‑Fetoprotein/AFP Monoclonal Antibody (Catalog # <a class=

MAB5369) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse alpha ‑Fetoprotein/AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5369) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF5369U-100UG
Alexa Fluor 405	AF5369V-100UG
Alexa Fluor 488	AF5369G-100UG
Alexa Fluor 532	AF5369X-100UG
Alexa Fluor 594	AF5369T-100UG
Alexa Fluor 647	AF5369R-100UG
Alexa Fluor 700	AF5369N-100UG
Alexa Fluor 750	AF5369S-100UG
Alexa Fluor Plus 405	AF5369AFP405-100UG
Alexa Fluor Plus 488	AF5369AFP488-100UG
Alexa Fluor Plus 555	AF5369AFP555-100UG
Alexa Fluor Plus 594	AF5369AFP594-100UG
Alexa Fluor Plus 647	AF5369AFP647-100UG
Alexa Fluor Plus 680	AF5369AFP680-100UG
Allophycocyanin	AF5369A
Biotin	BAF5369
CoraFluor 1	AF5369CL1
DyLight 350	AF5369D
DyLight 405	AF5369E
DyLight 488	AF5369K
DyLight 550	AF5369L
DyLight 594	AF5369M
DyLight 650	AF5369W
DyLight 680	AF5369Y
DyLight 755	AF5369Z
FITC	AF5369F
HRP	AF5369H
Janelia Fluor 525	AF5369JF525
Janelia Fluor 549	AF5369I
Janelia Fluor 646	AF5369J
Janelia Fluor 669	AF5369JF669
PE	AF5369P
PerCP	AF5369C
mFluor Violet 450 SE	AF5369MFV450
mFluor Violet 500 SE	AF5369MFV500
mFluor Violet 610 SE	AF5369MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF5369-SP

compare

(select up to 3 products from this page to compare)

AF1369

alpha-Fetoprotein/AFP Antibody [Unconjugated]

(1 Review)

(6 Publications)

100 μg

AF1369

Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of human liver tissue and H4‑II‑E‑C3 rat hepatoma cell line. PVDF membrane was probed with 0.2 µg/mL of Chicken Anti-Human/Rat alpha ‑Fetoprotein/ AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1369) followed by HRP-conjugated Anti-Chicken IgY Secondary Antibody. A specific band was detected for alpha ‑Fetoprotein/AFP at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using <a class=

Immunoblot Buffer Group 1." alt="Western blot shows lysates of human liver tissue and H4‑II‑E‑C3 rat hepatoma cell line. PVDF membrane was probed with 0.2 µg/mL of Chicken Anti-Human/Rat alpha ‑Fetoprotein/ AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1369) followed by HRP-conjugated Anti-Chicken IgY Secondary Antibody. A specific band was detected for alpha ‑Fetoprotein/AFP at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL016) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

a-Fetoprotein/AFP was detected in immersion fixed BG01V human embryonic stem cells using Chicken Anti-Human/Rat a-Fetoprotein/AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Chicken IgY Secondary Antibody (red; Catalog # <a class=

NL016) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

alpha ‑Fetoprotein/AFP was detected in immersion fixed paraffin-embedded sections of human liver cancer using 15 µg/mL Chicken anti-Human/ Rat alpha ‑Fetoprotein/AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1369) overnight at 4 °C. Tissue was stained (brown) and counterstained with hematoxylin (blue). View our protocol for <a class=

Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

NL018) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

NL018) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

Reactivity:

Hu, Rt

Species Glossary

Reactivity: Hu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Chicken Polyclonal

Host: Chicken Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF1369

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF1369-SP

compare

(select up to 3 products from this page to compare)

MAFP00

Mouse alpha-Fetoprotein/AFP Quantikine ELISA Kit

(17 Publications)

1 Kit

MAFP00

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

compare

(select up to 3 products from this page to compare)

NBP2-32914

alpha-Fetoprotein/AFP Antibody (C3)

(1 Publication)

0.1 mg

NBP2-32914

Flow (Intracellular): alpha-Fetoprotein/AFP Antibody (C3) [NBP2-32914] - An intracellular stain was performed on HepG2 cells with alpha-Fetoprotein/AFP Antibody [C3] NBP2-34577AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700. Image using the Alexa Fluor 700 Azide and BSA Free format of this antibody.

Reactivity:

Hu, Po, Ca, Pm, Bv(-), Mu(-), Rt(-)

Species Glossary

Reactivity: Hu, Po, Ca, Pm, Bv(-), Mu(-), Rt(-)

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-34577AF350
Alexa Fluor 405	NBP2-34577AF405
Alexa Fluor 488	NBP2-34577AF488
Alexa Fluor 532	NBP2-34577AF532
Alexa Fluor 594	NBP2-34577AF594
Alexa Fluor 647	NBP2-34577AF647
Alexa Fluor 700	NBP2-34577AF700
Alexa Fluor 750	NBP2-34577AF750
Alexa Fluor Plus 405	NBP2-34577AFP405
Alexa Fluor Plus 488	NBP2-34577AFP488
Alexa Fluor Plus 555	NBP2-34577AFP555
Alexa Fluor Plus 594	NBP2-34577AFP594
Alexa Fluor Plus 647	NBP2-34577AFP647
Alexa Fluor Plus 680	NBP2-34577AFP680
Allophycocyanin	NBP2-34577APC
Biotin	NBP2-34577B
CoraFluor 1	NBP2-34577CL1
DyLight 350	NBP2-34577UV
DyLight 405	NBP2-34577V
DyLight 488	NBP2-34577G
DyLight 550	NBP2-34577R
DyLight 594	NBP2-34577DL594
DyLight 650	NBP2-34577C
DyLight 680	NBP2-34577FR
DyLight 755	NBP2-34577IR
FITC	NBP2-34577F
HRP	NBP2-34577H
Janelia Fluor 525	NBP2-34577JF525
Janelia Fluor 549	NBP2-34577JF549
Janelia Fluor 646	NBP2-34577JF646
Janelia Fluor 669	NBP2-34577JF669
PE	NBP2-34577PE
PerCP	NBP2-34577PCP
mFluor Violet 450 SE	NBP2-34577MFV450
mFluor Violet 500 SE	NBP2-34577MFV500
mFluor Violet 610 SE	NBP2-34577MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
IHC-Prediluted	NBP2-45009
Azide and BSA Free	NBP2-34577-0.1mg

compare

(select up to 3 products from this page to compare)

H00000174-M01

alpha-Fetoprotein/AFP Antibody (1G7) - Azide and BSA Free

(9 Publications)

0.1 mg

H00000174-M01

Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01] - Immunoperoxidase of monoclonal antibody to AFP ( Cat # H00000174-M01 ) on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]

Western Blot: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01] - AFP monoclonal antibody (M01), clone 1G7 Analysis of AFP expression in HepG2.

Immunocytochemistry/Immunofluorescence: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01] - Analysis of monoclonal antibody to AFP on HepG2 cell. Antibody concentration 30 ug/ml.

Immunoprecipitation: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01] - Analysis of AFP transfected lysate using anti-AFP monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with AFP MaxPab rabbit polyclonal antibody.

ELISA: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01] - Detection limit for recombinant GST tagged AFP is approximately 0.03ng/ml as a capture antibody.

Reactivity:

Hu, Po

Species Glossary

Reactivity: Hu, Po

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

compare

(select up to 3 products from this page to compare)

DAFP00

Human alpha-Fetoprotein Quantikine ELISA Kit

(12 Publications)

1 Kit

DAFP00

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

compare

(select up to 3 products from this page to compare)

NBP1-76275

alpha-Fetoprotein/AFP Antibody - BSA Free

(6 Publications)

0.1 mg

NBP1-76275

Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody [NBP1-76275] - Rat liver tissue with AFP antibody at 10 ug/mL.

Immunohistochemistry: alpha-Fetoprotein/AFP Antibody - BSA Free [NBP1-76275] - Immunohistochemistry of alpha-Fetoprotein/AFP in mouse liver tissue with alpha-Fetoprotein/AFP antibody at 2.5 u/ml.

Reactivity:

Hu, Mu, Rt, Po

Species Glossary

Reactivity: Hu, Mu, Rt, Po

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-76275AF350
Alexa Fluor 405	NBP1-76275AF405
Alexa Fluor 488	NBP1-76275AF488
Alexa Fluor 532	NBP1-76275AF532
Alexa Fluor 594	NBP1-76275AF594
Alexa Fluor 647	NBP1-76275AF647
Alexa Fluor 700	NBP1-76275AF700
Alexa Fluor 750	NBP1-76275AF750
Alexa Fluor Plus 405	NBP1-76275AFP405
Alexa Fluor Plus 488	NBP1-76275AFP488
Alexa Fluor Plus 555	NBP1-76275AFP555
Alexa Fluor Plus 594	NBP1-76275AFP594
Alexa Fluor Plus 647	NBP1-76275AFP647
Alexa Fluor Plus 680	NBP1-76275AFP680
Allophycocyanin	NBP1-76275APC
Biotin	NBP1-76275B
CoraFluor 1	NBP1-76275CL1
DyLight 350	NBP1-76275UV
DyLight 405	NBP1-76275V
DyLight 488	NBP1-76275G
DyLight 550	NBP1-76275R
DyLight 594	NBP1-76275DL594
DyLight 650	NBP1-76275C
DyLight 680	NBP1-76275FR
DyLight 755	NBP1-76275IR
FITC	NBP1-76275F
HRP	NBP1-76275H
Janelia Fluor 525	NBP1-76275JF525
Janelia Fluor 549	NBP1-76275JF549
Janelia Fluor 646	NBP1-76275JF646
Janelia Fluor 669	NBP1-76275JF669
PE	NBP1-76275PE
PerCP	NBP1-76275PCP
mFluor Violet 450 SE	NBP1-76275MFV450
mFluor Violet 500 SE	NBP1-76275MFV500
mFluor Violet 610 SE	NBP1-76275MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-76275-0.025mg

compare

(select up to 3 products from this page to compare)

NBP2-03084

alpha-Fetoprotein/AFP Antibody (OTI2A9)

0.1 ml

NBP2-03084

Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody (OTI2A9) [NBP2-03084] - Staining of paraffin-embedded Human liver tissue using anti-Alpha 1 Fetoprotein mouse monoclonal antibody.

Flow Cytometry: alpha-Fetoprotein/AFP Antibody (OTI2A9) [NBP2-03084] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Alpha 1 Fetoprotein antibody, and then analyzed by flow cytometry.

Western Blot: alpha-Fetoprotein/AFP Antibody (OTI2A9) [NBP2-03084] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Alpha 1 Fetoprotein (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Alpha 1 Fetoprotein.

Flow Cytometry: alpha-Fetoprotein/AFP Antibody (OTI2A9) [NBP2-03084] - Analysis of Jurkat cells, using anti-Alpha 1 Fetoprotein antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Reactivity:

Hu, Ca, Pm, Pm

Species Glossary

Reactivity: Hu, Ca, Pm, Pm

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-70103AF350
Alexa Fluor 405	NBP2-70103AF405
Alexa Fluor 488	NBP2-70103AF488
Alexa Fluor 532	NBP2-70103AF532
Alexa Fluor 594	NBP2-70103AF594
Alexa Fluor 647	NBP2-70103AF647
Alexa Fluor 700	NBP2-70103AF700
Alexa Fluor 750	NBP2-70103AF750
Alexa Fluor Plus 405	NBP2-70103AFP405
Alexa Fluor Plus 488	NBP2-70103AFP488
Alexa Fluor Plus 555	NBP2-70103AFP555
Alexa Fluor Plus 594	NBP2-70103AFP594
Alexa Fluor Plus 647	NBP2-70103AFP647
Alexa Fluor Plus 680	NBP2-70103AFP680
Allophycocyanin	NBP2-70103APC
Biotin	NBP2-70103B
CoraFluor 1	NBP2-70103CL1
DyLight 350	NBP2-70103UV
DyLight 405	NBP2-70103V
DyLight 488	NBP2-70103G
DyLight 550	NBP2-70103R
DyLight 594	NBP2-70103DL594
DyLight 650	NBP2-70103C
DyLight 680	NBP2-70103FR
DyLight 755	NBP2-70103IR
FITC	NBP2-70103F
HRP	NBP2-70103H
Janelia Fluor 525	NBP2-70103JF525
Janelia Fluor 549	NBP2-70103JF549
Janelia Fluor 646	NBP2-70103JF646
Janelia Fluor 669	NBP2-70103JF669
PE	NBP2-70103PE
PerCP	NBP2-70103PCP
mFluor Violet 450 SE	NBP2-70103MFV450
mFluor Violet 500 SE	NBP2-70103MFV500
mFluor Violet 610 SE	NBP2-70103MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-70103

compare

(select up to 3 products from this page to compare)

NBP2-32915

alpha-Fetoprotein/AFP Antibody (SPM334)

(1 Publication)

0.1 mg

NBP2-32915

Reactivity:

Hu, Po, Ca, Pm, Bv(-), Mu(-), Rt(-)

Species Glossary

Reactivity: Hu, Po, Ca, Pm, Bv(-), Mu(-), Rt(-)

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-37754AF350
Alexa Fluor 405	NBP2-37754AF405
Alexa Fluor 488	NBP2-37754AF488
Alexa Fluor 532	NBP2-37754AF532
Alexa Fluor 594	NBP2-37754AF594
Alexa Fluor 647	NBP2-37754AF647
Alexa Fluor 700	NBP2-37754AF700
Alexa Fluor 750	NBP2-37754AF750
Alexa Fluor Plus 405	NBP2-37754AFP405
Alexa Fluor Plus 488	NBP2-37754AFP488
Alexa Fluor Plus 555	NBP2-37754AFP555
Alexa Fluor Plus 594	NBP2-37754AFP594
Alexa Fluor Plus 647	NBP2-37754AFP647
Alexa Fluor Plus 680	NBP2-37754AFP680
Allophycocyanin	NBP2-37754APC
Biotin	NBP2-37754B
CoraFluor 1	NBP2-37754CL1
DyLight 350	NBP2-37754UV
DyLight 405	NBP2-37754V
DyLight 488	NBP2-37754G
DyLight 550	NBP2-37754R
DyLight 594	NBP2-37754DL594
DyLight 650	NBP2-37754C
DyLight 680	NBP2-37754FR
DyLight 755	NBP2-37754IR
FITC	NBP2-37754F
HRP	NBP2-37754H
Janelia Fluor 525	NBP2-37754JF525
Janelia Fluor 549	NBP2-37754JF549
Janelia Fluor 646	NBP2-37754JF646
Janelia Fluor 669	NBP2-37754JF669
PE	NBP2-37754PE
PerCP	NBP2-37754PCP
mFluor Violet 450 SE	NBP2-37754MFV450
mFluor Violet 500 SE	NBP2-37754MFV500
mFluor Violet 610 SE	NBP2-37754MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-37754-0.1mg
IHC-Prediluted	NBP2-45010

compare

(select up to 3 products from this page to compare)

MAB13692

alpha-Fetoprotein/AFP Antibody (189508) - Azide and BSA Free

100 μg

MAB13692

Simple Western: alpha-Fetoprotein/AFP Antibody (189508) [MAB13692] - Lane view shows a specific band for Fetoprotein-AFP in 0.2 mg/mL of HepG2 and HepB3 lysate. Antibody at 5 ug/mL. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	MAB13692AF350
Alexa Fluor 405	MAB13692AF405
Alexa Fluor 488	MAB13692AF488
Alexa Fluor 532	MAB13692AF532
Alexa Fluor 594	MAB13692AF594
Alexa Fluor 647	MAB13692AF647
Alexa Fluor 700	MAB13692AF700
Alexa Fluor 750	MAB13692AF750
Alexa Fluor Plus 405	MAB13692AFP405
Alexa Fluor Plus 488	MAB13692AFP488
Alexa Fluor Plus 555	MAB13692AFP555
Alexa Fluor Plus 594	MAB13692AFP594
Alexa Fluor Plus 647	MAB13692AFP647
Alexa Fluor Plus 680	MAB13692AFP680
Allophycocyanin	MAB13692APC
Biotin	MAB13692B
DyLight 350	MAB13692UV
DyLight 405	MAB13692V
DyLight 488	MAB13692G
DyLight 550	MAB13692R
DyLight 594	MAB13692DL594
DyLight 650	MAB13692C
DyLight 680	MAB13692FR
DyLight 755	MAB13692IR
HRP	MAB13692H
PE	MAB13692PE

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	MAB13692-SP

compare

(select up to 3 products from this page to compare)

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

AFP Products By Gene Name

alpha-Fetoprotein/AFP Antibody (189502) [Unconjugated]

alpha-Fetoprotein/AFP Antibody (189506) [Unconjugated]

alpha-Fetoprotein/AFP Antibody

alpha-Fetoprotein/AFP Antibody [Unconjugated]

Mouse alpha-Fetoprotein/AFP Quantikine ELISA Kit

alpha-Fetoprotein/AFP Antibody (C3)

alpha-Fetoprotein/AFP Antibody (1G7) - Azide and BSA Free

Human alpha-Fetoprotein Quantikine ELISA Kit

alpha-Fetoprotein/AFP Antibody - BSA Free

alpha-Fetoprotein/AFP Antibody (OTI2A9)

alpha-Fetoprotein/AFP Antibody (SPM334)

alpha-Fetoprotein/AFP Antibody (189508) - Azide and BSA Free