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HAF018). A specific band was detected for a-Fetoprotein/AFP at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_lightbox" target="_blank">
HAF018). A specific band was detected for a-Fetoprotein/AFP at approximately 70 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
MAB002, open histogram) followed by PE-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0102B). No staining in the a-Fetoprotein/AFP knockout HepG2 cell line was observed. View our protocol for Staining Membrane-associated Proteins." class="big_lightbox" target="_blank">
MAB004, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_lightbox" target="_blank">
HAF019). A specific band was detected for a-Fetoprotein/AFP at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_lightbox" target="_blank">
MAB5369) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse ?‑Fetoprotein/AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5369) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
MAB5369) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse alpha ‑Fetoprotein/AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5369) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
Alpha 1 Fetoprotein encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.
![Western Blot alpha-Fetoprotein/AFP Antibody (189502) [Unconjugated]](http://images.novusbio.com/images/antibody/alphaFetoprotein_MAB1368_Western_Blot_20276.jpg)
![Immunocytochemistry alpha-Fetoprotein/AFP Antibody (189506) [Unconjugated]](http://images.novusbio.com/images/antibody/alphaFetoprotein_MAB1369_Immunocytochemistry__Immunofluorescence_17789.jpg)
![Sandwich ELISA alpha-Fetoprotein/AFP Antibody Pair [HRP]](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-Pair-[HRP]-Sandwich-ELISA-NBP2-79389-img0001.jpg)
![N/A alpha-Fetoprotein/AFP [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_alphaFetoprotein_MAFP00_ELISA_938.jpg)
![N/A alpha-Fetoprotein/AFP [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_alphaFetoprotein_DAFP00_ELISA_566.jpg)
![N/A alpha-Fetoprotein/AFP [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_alphaFetoprotein_DY1369_ELISA_1635.jpg)
![Immunocytochemistry alpha-Fetoprotein/AFP Antibody (189502) [Unconjugated]](http://images.novusbio.com/images/antibody/alphaFetoprotein_MAB1368_Immunocytochemistry__Immunofluorescence_18643.jpg)
![Intracellular Staining by Flow Cytometry alpha-Fetoprotein/AFP Antibody (189502) [Unconjugated]](http://images.novusbio.com/images/antibody/alphaFetoprotein_MAB1368_Flow_Cytometry_20263.jpg)
![Simple Western alpha-Fetoprotein/AFP Antibody (189502) [Unconjugated]](http://images.novusbio.com/fullsize/antibody/16253.jpg)
![Knockout Validated alpha-Fetoprotein/AFP Antibody (189502) [Unconjugated]](http://images.novusbio.com/images/antibody/alphaFetoprotein_MAB1368_Knockout_Validated_21503.jpg)
![Knockout Validated alpha-Fetoprotein/AFP Antibody (189502) [Unconjugated]](http://images.novusbio.com/images/antibody/alphaFetoprotein_MAB1368_Knockout_Validated_22402.jpg)
![Immunocytochemistry/ Immunofluorescence alpha-Fetoprotein/AFP Antibody (189502) [Unconjugated]](http://images.novusbio.com/fullsize/mab1368_human-mouse-alpha-fetoprotein-mab-clone-189502-41202410325519.jpg)
![Immunocytochemistry/ Immunofluorescence alpha-Fetoprotein/AFP Antibody (189502) [Unconjugated]](http://images.novusbio.com/fullsize/mab1368_human-mouse-alpha-fetoprotein-mab-clone-189502-41202412514817.jpg)
![Immunohistochemistry alpha-Fetoprotein/AFP Antibody (189502) [Unconjugated]](http://images.novusbio.com/fullsize/mab1368_human-mouse-alpha-fetoprotein-mab-clone-189502-41202412512672.jpg)
![Immunocytochemistry/ Immunofluorescence alpha-Fetoprotein/AFP Antibody (189502) [Unconjugated]](http://images.novusbio.com/fullsize/mab1368_human-mouse-alpha-fetoprotein-mab-clone-189502-41202412514844.jpg)
![Intracellular Staining by Flow Cytometry alpha-Fetoprotein/AFP Antibody (189506) [Unconjugated]](http://images.novusbio.com/images/antibody/alphaFetoprotein_MAB1369_Flow_Cytometry_20916.jpg)
![Knockout Validated alpha-Fetoprotein/AFP Antibody (189506) [Unconjugated]](http://images.novusbio.com/images/antibody/alphaFetoprotein_MAB1369_Knockout_Validated_22418.jpg)
![N/A alpha-Fetoprotein/AFP [HRP]](http://images.novusbio.com/fullsize/elisa/alphaFetoprotein_MAFP00_ELISA_497.jpg)
![N/A alpha-Fetoprotein/AFP [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_alphaFetoprotein_DAFP00_ELISA_567.jpg)
![N/A alpha-Fetoprotein/AFP [HRP]](http://images.novusbio.com/fullsize/elisa/alpha-Fetoprotein_DAFP00_ELISA_25.jpg)
