alpha-Fetoprotein/AFP Antibody (C3) [Biotin]

• Reactivity: Hu, Po, Ca, Pm, Bv(-), Mu(-), Rt(-)
• Applications: ELISA, Flow, IHC, CyTOF-ready, KO
• Clone: C3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Biotin

alpha-Fetoprotein/AFP Antibody (C3) [Biotin] Summary

• Description: This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
• Immunogen: Alpha feto protein (AFP) Purified from serum of a hepatoma patient (Uniprot: P02771)
• Localization: Cytoplasmic
• Marker: Hepatocellular/Germ Cell Tumor Marker
• Specificity: It recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as alpha-fetoprotein (AFP) (ISOBM TD-2 workshop). This monoclonal antibody is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: AFP
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Knockout Validated
  • Sandwich ELISA
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Reactivity Notes

Does not react with Bovine, Mouse or Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for alpha-Fetoprotein/AFP Antibody (C3) [Biotin]

• AFP
• Alpha-1-fetoprotein
• alpha-fetoglobulin
• alpha-Fetoprotein
• DSCAM2
• FETA
• HP
• HPAFP

Background

Alpha 1 Fetoprotein encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for alpha-Fetoprotein/AFP Antibody (NBP2-34577B)(1)

Publication using NBP2-34577B

• Dadfar S, Sekula-Neuner S, Trouillet V, et al. Evaluation of click chemistry microarrays for immunosensing of alpha-fetoprotein (AFP) Beilstein J. Nanotechnol. 2019-12-16 [PMID: 31921529] (S-ELISA)

Bioinformatics

• Gene Symbol: AFP