alpha-Fetoprotein/AFP Antibody (C3) [Janelia Fluor® 549]

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Product Details

Summary
Reactivity Hu, Po, Ca, Pm, Bv(-), Ca(-), Mu(-), Rt(-)Species Glossary
Applications Flow, ICC/IF, IHC, IHC-Fr, IHC-P, KO
Clone
C3
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 549

Order Details

alpha-Fetoprotein/AFP Antibody (C3) [Janelia Fluor® 549] Summary

Immunogen
Alpha fetoprotein (AFP) purified from serum of a hepatoma patient
Localization
Cytoplasmic
Marker
Hepatocellular/Germ Cell Tumor Marker
Specificity
It recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as alpha-fetoprotein (AFP) (ISOBM TD-2 workshop). This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
AFP
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Knockout Validated
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for alpha-Fetoprotein/AFP Antibody (C3) [Janelia Fluor® 549]

  • AFP
  • Alpha-1-fetoprotein
  • alpha-fetoglobulin
  • alpha-Fetoprotein
  • DSCAM2
  • FETA
  • HP
  • HPAFP

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Po, Ca, Pm, Bv(-), Ca(-), Mu(-), Rt(-)
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, KO

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-34577AF350
Alexa Fluor 405 NBP2-34577AF405
Alexa Fluor 488 NBP2-34577AF488
Alexa Fluor 532 NBP2-34577AF532
Alexa Fluor 594 NBP2-34577AF594
Alexa Fluor 647 NBP2-34577AF647
Alexa Fluor 700 NBP2-34577AF700
Alexa Fluor 750 NBP2-34577AF750
Allophycocyanin NBP2-34577APC
Biotin NBP2-34577B
DyLight 350 NBP2-34577UV
DyLight 405 NBP2-34577V
DyLight 488 NBP2-34577G
DyLight 550 NBP2-34577R
DyLight 594 NBP2-34577DL594
DyLight 650 NBP2-34577C
DyLight 680 NBP2-34577FR
DyLight 755 NBP2-34577IR
FITC NBP2-34577F
HRP NBP2-34577H
Janelia Fluor 549 NBP2-34577JF549
Janelia Fluor 646 NBP2-34577JF646
PE NBP2-34577PE
PerCP NBP2-34577PCP

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Bioinformatics Tool for alpha-Fetoprotein/AFP Antibody (NBP2-34577JF549)

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