Alpha Actinin 4 Antibody

Images

 
Western Blot: Alpha Actinin 4 Antibody [NBP1-89818] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: Alpha Actinin 4 Antibody [NBP1-89818] - Immunofluorescent staining of human cell line A-431 shows localization to nuclear bodies.
Immunohistochemistry-Paraffin: Alpha Actinin 4 Antibody [NBP1-89818] - Staining of human liver shows strong cytoplasmic and membranous positivity in bile duct cells.
Western Blot: Alpha Actinin 4 Antibody [NBP1-89818] - Analysis in human cell line A-549.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Alpha Actinin 4 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:MFIVHTIEEIEGLISAHDQFKSTLPDADREREAILAIHKEAQRIAESNHIKLSGSNPYTTVTPQIINSKWEKVQQLVPKRDHALLEEQSKQQSNEHLRRQFASQANVVGPWIQTKMEEIGRISIEMNGTLE
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ACTN4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:500
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:1000-1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Alpha Actinin 4 Protein (NBP1-89818PEP)
Publications
Read Publication using NBP1-89818.

Reactivity Notes

Reactivity reported in scientific literature (PMID: 22361696)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Alpha Actinin 4 Antibody

  • actinin alpha4 isoform
  • actinin, alpha 4
  • ACTININ-4
  • alpha-actinin-4
  • DKFZp686K23158
  • F-actin cross-linking protein
  • focal segmental glomerulosclerosis 1
  • FSGS
  • FSGS1
  • Non-muscle alpha-actinin 4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Alpha Actinin 4 Antibody (NBP1-89818)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ACTN4