alpha-Actinin 1 Antibody (OTI7A4)

Images

 
Western Blot: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-ACTN1 monoclonal antibody (1:200).
Immunocytochemistry/ Immunofluorescence: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of Hela cells transiently transfected by pCMV6-ENTRY Alpha Actinin 1.
Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of paraffin-embedded Human Ovary tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.
Western Blot: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Alpha Actinin 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Western Blot: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251] - Analysis of extracts (10ug) from 8 different cell lines by using anti-ACTN1 monoclonal antibody at 1:200 dilution.
Immunocytochemistry/ Immunofluorescence: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of HeLa cells using anti-Alpha Actinin 1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of paraffin-embedded Human bladder tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of paraffin-embedded Human breast tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of paraffin-embedded Human Kidney tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of paraffin-embedded Human liver tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.
Knockout Validated: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, and ACTN1-Knockout HeLa cells (KO) were separated by SDS-PAGE and ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, KO
Clone
OTI7A4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.88 mg/ml

alpha-Actinin 1 Antibody (OTI7A4) Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 650-893 of human alpha-actinin (NP_001093) produced in E.coli.
Marker
Z-disk Marker
Specificity
This antibody is specific for Homo sapiens actinin, alpha 1 (ACTN1), transcript variant 2.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ACTN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50
  • Knockout Validated
Theoretical MW
102.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
alpha-Actinin 1 Knockout HeLa Cell Lysate
Publications
Read Publications using
NBP1-48251 in the following applications:

  • IHC
    2 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27512039). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.88 mg/ml
Purity
Immunogen affinity purified

Alternate Names for alpha-Actinin 1 Antibody (OTI7A4)

  • actinin 1 smooth muscle
  • actinin, alpha 1
  • ACTN1
  • alpha Actinin 1
  • alphaActinin 1
  • alpha-Actinin 1
  • Alpha-actinin cytoskeletal isoform
  • alpha-actinin-1
  • BDPLT15
  • F-actin cross-linking protein
  • FLJ40884
  • FLJ54432
  • Non-muscle alpha-actinin-1

Background

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for alpha-Actinin 1 Antibody (NBP1-48251)(2)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for alpha-Actinin 1 Antibody (NBP1-48251) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-70078AF350
Alexa Fluor 405 NBP2-70078AF405
Alexa Fluor 488 NBP2-70078AF488
Alexa Fluor 532 NBP2-70078AF532
Alexa Fluor 594 NBP2-70078AF594
Alexa Fluor 647 NBP2-70078AF647
Alexa Fluor 700 NBP2-70078AF700
Alexa Fluor 750 NBP2-70078AF750
Biotin NBP2-70078B
DyLight 350 NBP2-70078UV
DyLight 405 NBP2-70078V
DyLight 488 NBP2-70078G
DyLight 550 NBP2-70078R
DyLight 594 NBP2-70078DL594
DyLight 650 NBP2-70078C
DyLight 680 NBP2-70078FR
DyLight 755 NBP2-70078IR
FITC NBP2-70078F
HRP NBP2-70078H
Janelia Fluor 549 NBP2-70078JF549
Janelia Fluor 646 NBP2-70078JF646

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Bioinformatics

Gene Symbol ACTN1
Entrez