alpha-Actinin 1 Antibody (3F1) - Azide and BSA Free

Western Blot: alpha-Actinin 1 Antibody (3F1) [H00000087-M01] - Analysis of ACTN1 expression in transfected 293T cell line by ACTN1 monoclonal antibody (M01), clone 3F1.Lane 1: ACTN1 transfected lysate(103.1 KDa).Lane 2: Non-transfected lysate.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 3F1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

alpha-Actinin 1 Antibody (3F1) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: ACTN1 (NP_001093, 543 a.a. ~ 639 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFGAQA
• Marker: Z-disk Marker
• Specificity: ACTN1 - actinin, alpha 1
• Isotype: IgG3 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACTN1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for alpha-Actinin 1 Antibody (3F1) - Azide and BSA Free

• actinin 1 smooth muscle
• actinin, alpha 1
• ACTN1
• alpha Actinin 1
• alphaActinin 1
• alpha-Actinin 1
• Alpha-actinin cytoskeletal isoform
• alpha-actinin-1
• BDPLT15
• F-actin cross-linking protein
• FLJ40884
• FLJ54432
• Non-muscle alpha-actinin-1

Background

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for alpha-Actinin 1 Antibody (H00000087-M01)(2)

Publications using H00000087-M01

• Sakane A, Abdallah AA, Nakano K et al. Rab13 Small G Protein and Junctional Rab13-binding Protein (JRAB) Orchestrate Actin Cytoskeletal Organization during Epithelial Junctional Development. J Biol Chem. 2012-10-24 [PMID: 23100251]
• Quick Q, Skalli O. alpha-Actinin 1 and alpha-actinin 4: Contrasting roles in the survival, motility, and RhoA signaling of astrocytoma cells. Exp Cell Res;316(7):1137-47. 2010-04-15 [PMID: 20156433]

Bioinformatics

• Gene Symbol: ACTN1
• Entrez:
  • 87(Human)
• OMIM:
  • 102575(Human)
• Uniprot:
  • NP_001093(Human)