ALK/CD246 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: ALK/CD246 Antibody [NBP2-48518] - Staining of human cell line RH-30 shows localization to plasma membrane. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: ALK/CD246 Antibody [NBP2-48518] - Staining of human cerebral cortex shows cytoplasmic positivity in neurons.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ALK/CD246 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ALK/CD246 Antibody - BSA Free (NBP2-48518) is a polyclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: IFGTGHSSLESPTNMPSPSPDYFTWNLTWIMKDSFPFLSHRSRYGLECSFDFPCELEYSPPLHDLRNQSWSWRRIPSEEASQMDLLDGPGAERSKEMPRGSFLLLNTSADSKHTILS
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ALK
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  ALK/CD246 Recombinant Protein Antigen (NBP2-48518PEP)

Reactivity Notes

Mouse (84%), Rat (83%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for ALK/CD246 Antibody - BSA Free

• ALK tyrosine kinase receptor
• ALK
• anaplastic lymphoma kinase (Ki-1)
• Anaplastic lymphoma kinase
• anaplastic lymphoma receptor tyrosine kinase
• CD246 antigen
• CD246
• EC 2.7.10.1
• Ki-1
• NBLST3
• Tcrz

Background

ALK is a novel receptor protein-tyrosine kinase having a putative transmembrane domain and an extracellular domain. The 2;5 chromosomal translocation occurs in most anaplastic large-cell non-Hodgkin's lymphomas arising from activated T lymphocytes. This rearrangement was shown to fuse the NPM nucleolar phosphoprotein gene on chromosome 5q35 to a previously unidentified protein tyrosine kinase gene, ALK, on chromosome 2p23 (1). In the predicted hybrid protein, the amino terminus of NPM is linked to the catalytic domain of ALK. Immunoblotting with anti-ALK antibody shows that ALK is highly expressed in the neonatal brain (2). Also expressed in the small intestine, testis, and brain but not in normal lymphoid cells, ALK shows greatest sequence similarity to the insulin receptor subfamily of kinases. Unscheduled expression of the truncated ALK may contribute to malignant transformation in these lymphomas (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ALK