Recombinant Human Aldolase A Protein (NBP1-40395)

Images

 
SDS-Page: Recombinant Human Aldolase A Protein [NBP1-40395] - Aldolase A, 41.5 kDa (384aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human Aldolase A Protein Summary

Description
A recombinant protein with a His-tag corresponding to amino acids 1 to 364 of Human Aldolase A

Source: E. coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MPYQYPALTP EQKKELSDIA HRIVAPGKGI LAADESTGSI AKRLQSIGTE NTEENRRFYR QLLLTADDRV NPCIGGVILF HETLYQKADD GRPFPQVIKS KGGVVGIKVD KGVVPLAGTN GETTTQGLDG LSERCAQYKK DGADFAKWRC VLKIGEHTPS ALAIMENANV LARYASICQQ NGIVPIVEPE ILPDGDHDLK RCQYVTEKVL AAVYKALSDH HIYLEGTLLK PNMVTPGHAC TQKFSHEEIA MATVTALRRT VPPAVTGITF LSGGQSEEEA SINLNAINKC PLLKPWALTF SYGRALQASA LKAWGGKKEN LKAAQEEYVK RALANSLACQ GKYTPSGQAG AAASESLFVS NHAY
Preparation
Method
E.coli
Details of Functionality
Specific activity is > 10 units/mg, one unit will convert 1.0 umol of fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehydes 3-phosphate per minute at pH 7.5 at 37C.
Protein/Peptide Type
Biologically Active Protein
Gene
ALDOA
Purity
>95% pure by SDS-PAGE

Applications/Dilutions

Theoretical MW
41.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH8.0) containing 100mM NaCl, 10% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95% pure by SDS-PAGE

Notes

The purity of this protein is > 95% by SDS-PAGE. Molecular weight is 41.5 kDa (384aa), confirmed by MALDI-TOF.

Alternate Names for Recombinant Human Aldolase A Protein

  • ALDA
  • aldolase A, fructose-bisphosphate
  • EC 4.1.2.13
  • fructose-1,6-bisphosphate triosephosphate-lyase
  • fructose-bisphosphate aldolase A
  • GSD12
  • Lung cancer antigen NY-LU-1
  • MGC10942
  • MGC17716
  • MGC17767
  • Muscle-type aldolase

Background

Fructose bisphosphate aldolase A, also known as Aldolase A is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. It is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Deficiency has been associated with myopathy and hemolytic anemia. Recombinant human Aldolase A, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-02164
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NB300-221
Species: Hu, Mu, Rt, Po, Av, Bv, Ch, Eq, Ha, Op, Pm, Sh, Ze
Applications: WB, Simple Western, ChIP, ICC/IF, IHC, IP
H00055821-D01P
Species: Hu, Mu
Applications: WB, ELISA
NBP2-25147
Species: Hu, Mu, Rt, Bv, Xp
Applications: WB, Simple Western, ICC/IF
NBP1-48336
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-31470
Species: Hu, Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
AF3589
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC
NBP2-19784
Species: Hu, Mu, Rt, Ye
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP2-32433
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-30249
Species: Hu, Mu, Rt, Xp
Applications: WB, ELISA, Flow, ICC/IF
NBP2-02541
Species: Hu, Pm
Applications: WB, ICC/IF, IHC, IHC-P
NB100-105
Species: Hu, Mu, Rt, Po, Bv, Ft, Mk, Pm, Rb, Sh, Xp, Ye
Applications: WB, ChIP, ELISA, Flow, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP, TCS, LA
NB600-41532
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NB300-223
Species: Hu, Mu, Rt, Ca
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-48308
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NB100-65214
Species: Hu, Mu, Rt, Ma
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-24449
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow
AF2180
Species: Hu
Applications: WB, Simple Western, IP
H00006690-M01
Species: Hu
Applications: WB, ELISA, IHC-P, IP
NBP1-40395
Species: Hu
Applications: Func, PAGE

Publications for Aldolase A Biologically Active Protein (NBP1-40395) (0)

There are no publications for Aldolase A Biologically Active Protein (NBP1-40395).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Aldolase A Biologically Active Protein (NBP1-40395) (0)

There are no reviews for Aldolase A Biologically Active Protein (NBP1-40395). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

View specific protocols for Aldolase A Biologically Active Protein (NBP1-40395): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Aldolase A Biologically Active Protein (NBP1-40395) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Aldolase A Products

Related Products by Gene

Bioinformatics Tool for Aldolase A Biologically Active Protein (NBP1-40395)

Discover related pathways, diseases and genes to Aldolase A Biologically Active Protein (NBP1-40395). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Aldolase A Biologically Active Protein (NBP1-40395)

Discover more about diseases related to Aldolase A Biologically Active Protein (NBP1-40395).
 

Pathways for Aldolase A Biologically Active Protein (NBP1-40395)

View related products by pathway.

PTMs for Aldolase A Biologically Active Protein (NBP1-40395)

Learn more about PTMs related to Aldolase A Biologically Active Protein (NBP1-40395).
 

Research Areas for Aldolase A Biologically Active Protein (NBP1-40395)

Find related products by research area.

Blogs on Aldolase A

There are no specific blogs for Aldolase A, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human Aldolase A Protein and receive a gift card or discount.

Bioinformatics

Gene Symbol ALDOA