Aldolase A Antibody (JM54-19)

Western Blot: Aldolase A Antibody (JM54-19) [NBP2-75402] - Analysis of Aldolase on Zebrafish tissue lysates using anti-Aldolase antibody at 1/200 dilution.

Immunocytochemistry/Immunofluorescence: Aldolase A Antibody (JM54-19) [NBP2-75402] - Staining Aldolase in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Aldolase A Antibody (JM54-19) [NBP2-75402] - Analysis of paraffin-embedded rat skeletal muscle tissue using anti-Aldolase antibody. Counter stained with hematoxylin.

Western Blot: Aldolase A Antibody (JM54-19) [NBP2-75402] - Analysis of Aldolase on different lysates using anti-Aldolase antibody at 1/1,000 dilution.Positive control:Lane 1: A549 Lane 2: Hela Lane 3: Mouse liver Lane 4: Rat liverLane 5: Rat spleen

Immunocytochemistry/Immunofluorescence: Aldolase A Antibody (JM54-19) [NBP2-75402] - Staining Aldolase in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Aldolase A Antibody (JM54-19) [NBP2-75402] - Staining Aldolase in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Aldolase A Antibody (JM54-19) [NBP2-75402] - Analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-Aldolase antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Aldolase A Antibody (JM54-19) [NBP2-75402] - Analysis of paraffin-embedded human pancreas tissue using anti-Aldolase antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Aldolase A Antibody (JM54-19) [NBP2-75402] - Analysis of paraffin-embedded mouse brain tissue using anti-Aldolase antibody. Counter stained with hematoxylin.

Product Discontinued

Aldolase A Antibody (JM54-19) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within N-terminal human Aldolase A . (SwissProt: P04075 Human; SwissProt: P05064 Mouse; SwissProt: P05065 Rat)
• Localization: Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ALDOA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 39 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Aldolase A Antibody (JM54-19)

• ALDA
• aldolase A, fructose-bisphosphate
• EC 4.1.2.13
• fructose-1,6-bisphosphate triosephosphate-lyase
• fructose-bisphosphate aldolase A
• GSD12
• Lung cancer antigen NY-LU-1
• MGC10942
• MGC17716
• MGC17767
• Muscle-type aldolase

Background

Aldolase A (fructose bisphosphate aldolase) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3 phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants which encode the same protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ALDOA