Aldo-keto Reductase 1C3/AKR1C3 Antibody

Western blot shows lysates of Huh-7 human hepatoma cell line, A549 human lung carcinoma cell line, and human liver tissue. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human Aldo-keto Reductase 1C3/AKR1C3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7678) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Aldo-keto Reductase 1C3/AKR1C3 at approximately 35-36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Aldo-keto Reductase 1C3/AKR1C3 Antibody Summary

• Immunogen: E. coli-derived recombinant human Aldo-keto Reductase 1C3/AKR1C3
  Met1-Tyr323
  Accession # P42330
• Specificity: Detects human Aldo-keto Reductase 1C3/AKR1C3 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human (rh) AKR1C1 is observed, and approximately 25% cross-reactivity with rhAKR1C4 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: AKR1C3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Aldo-keto Reductase 1C3/AKR1C3 Antibody

• 17-beta-hydroxysteroid dehydrogenase type 5
• 3-alpha-HSD type 2
• 3-alpha-hydroxysteroid dehydrogenase type 2
• AKR1C3
• Aldo-keto Reductase 1C3
• aldo-keto reductase family 1 member C3
• aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II)
• AldoketoReductase 1C3
• Chlordecone reductase homolog HAKRb
• DD3
• DD-3
• DD317-beta-HSD 5
• DDH1
• DDX
• Dihydrodiol dehydrogenase 3
• Dihydrodiol dehydrogenase type I
• dihydrodiol dehydrogenase X
• EC 1.1.1
• EC 1.1.1.112,3-alpha-HSD type II, brain
• EC 1.1.1.188
• EC 1.1.1.213
• EC 1.1.1.63
• EC 1.1.1.64
• EC 1.3.1.20
• HA1753
• HAKRB
• HAKRBtype IIb 3-alpha hydroxysteroid dehydrogenase
• HAKRe
• HSD17B5
• hydroxysteroid (17-beta) dehydrogenase 5
• Indanol dehydrogenase
• KIAA0119hluPGFS
• PGFS
• Prostaglandin F synthase
• Testosterone 17-beta-dehydrogenase 5
• Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase

Background

AKR1C3 (Aldo-Keto Reductase family 1 member C3; also 17-beta HSD 5, prostaglandin F synthase/PGFS and 3-alpha HSD type 2) is a 35-36 kDa member of the four gene 3-alpha HSD family, aldo-keto reductase superfamily of enzymes. It is expressed by multiple cell types, including prostate epithelium, T cells, and hepatocytes. AKR1C3 generates testosterone and progesterone, catalyzes the conversion of aldehydes and ketones into alcohols, and the mediates the reduction of prostaglandin D2 into PGF2. Human AKR1C3 is 323 amino acids (aa) in length. There are three potential isoform variants. One contains an alternative start site at Met120, a second shows a five aa substitution for aa 1-28, and a third possesses a 15 aa substitution for aa 124-323. Full-length human AKR1C3 shares 73% aa sequence identity with mouse AKR1C3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AKR1C3
• Uniprot:
  • P42330(Human)