Aldo-keto Reductase 1C3/AKR1C3 Antibody [CoraFluor™ 1]

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: CoraFluor 1

Aldo-keto Reductase 1C3/AKR1C3 Antibody [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: E. coli-derived recombinant human Aldo-keto Reductase 1C3/AKR1C3
  Met1-Tyr323
  Accession # P42330
• Specificity: Detects human Aldo-keto Reductase 1C3/AKR1C3 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human (rh) AKR1C1 is observed, and approximately 25% cross-reactivity with rhAKR1C4 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: AKR1C3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Aldo-keto Reductase 1C3/AKR1C3 Antibody [CoraFluor™ 1]

• 17-beta-hydroxysteroid dehydrogenase type 5
• 3-alpha-HSD type 2
• 3-alpha-hydroxysteroid dehydrogenase type 2
• AKR1C3
• Aldo-keto Reductase 1C3
• aldo-keto reductase family 1 member C3
• aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II)
• AldoketoReductase 1C3
• Chlordecone reductase homolog HAKRb
• DD3
• DD-3
• DD317-beta-HSD 5
• DDH1
• DDX
• Dihydrodiol dehydrogenase 3
• Dihydrodiol dehydrogenase type I
• dihydrodiol dehydrogenase X
• EC 1.1.1
• EC 1.1.1.112,3-alpha-HSD type II, brain
• EC 1.1.1.188
• EC 1.1.1.213
• EC 1.1.1.63
• EC 1.1.1.64
• EC 1.3.1.20
• HA1753
• HAKRB
• HAKRBtype IIb 3-alpha hydroxysteroid dehydrogenase
• HAKRe
• HSD17B5
• hydroxysteroid (17-beta) dehydrogenase 5
• Indanol dehydrogenase
• KIAA0119hluPGFS
• PGFS
• Prostaglandin F synthase
• Testosterone 17-beta-dehydrogenase 5
• Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase

Background

AKR1C3 encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ), and the oxidation of 9alpha,11beta-PGF2 to PGD2. It may play an important role in the pathogenesis of allergic diseases such as asthma, and may also have a role in controlling cell growth and/or differentiation. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AKR1C3