ALDH4A1 Antibody (002) - Azide and BSA Free

Western Blot: ALDH4A1 Antibody (002) [NBP2-90152] - Anti-ALDH4A1 rabbit monoclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: K562 Whole Cell Lysate. Lane C: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L(Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 62 kDa Observed band size: 62 kDa (We are unsure as to the identity of these extra bands.)

Immunoprecipitation: ALDH4A1 Antibody (002) [NBP2-90152] - ALDH4A1 was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate. Lane B:0.5 mg K562 Whole Cell Lysate. 2 uL anti-ALDH4A1 rabbit monoclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-ALDH4A1 rabbit monoclonal antibody,at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 62 kDa. Observed band size: 62 kDa.

• Reactivity: Hu
• Applications: WB, ELISA, IP
• Clone: 002
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ALDH4A1 Antibody (002) - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was obtained from a rabbit immunized with purified, recombinant Human ALDH4A1 (Accession#: AAH07581.1; Lys25-Gln563).
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ALDH4A1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:10000
  • Immunoprecipitation 1-4 uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for ALDH4A1 Antibody (002) - Azide and BSA Free

• aldehyde dehydrogenase 4 family, member A1
• Aldehyde dehydrogenase family 4 member A1
• ALDH4DKFZp779M035
• delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial
• EC 1.5.1.12
• mitochondrial delta-1-pyrroline 5-carboxylate dehydrogenase
• P5C dehydrogenase
• P5CD
• P5CDH

Background

ALDH4A1 belongs to the aldehyde dehydrogenase family of proteins. This enzyme is a mitochondrial matrix NAD-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate. Deficiency of this enzyme is associated with type II hyperprolinemia, an autosomal recessive disorder characterized by accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline. Two transcript variants encoding the same protein have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ALDH4A1 Antibody (NBP2-90152)(1)

Publication using NBP2-90152

• Kao Y, Huang LC, Hsu SY et al. The Effect of Disulfiram and Copper on Cellular Viability, ER Stress and ALDH Expression of Human Meningioma Cells Biomedicines Apr 12 2022 [PMID: 35453636]

Bioinformatics

• Gene Symbol: ALDH4A1