PRODH Antibody

Images

 
Immunohistochemistry-Paraffin: PRODH Antibody [NB100-2832] - (3.8ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

PRODH Antibody Summary

Immunogen
Peptide with sequence C-ERDGSGTNKRDKQYQ corresponding to internal region according to NP_057419.2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PRODH
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Immunohistochemistry 3 - 6 ug/ml
  • Immunohistochemistry-Paraffin 3 - 6 ug/ml
  • Peptide ELISA Detection limit 1:16000
Application Notes
WB: Preliminary experiments gave bands at approx. 85 kDa band and 25 kDa band in human brain lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 68.0 kDa band according to NP_057419.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). IHC-P: Cerebellum shows mitochondrial staining in smaller neurons.
Publications
Read Publication using NB100-2832.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PRODH Antibody

  • EC 1.5.99.8
  • FLJ33744
  • HSPOX2
  • mitochondrial
  • p53-induced gene 6 protein
  • PIG6MGC148078
  • POX2
  • PRODH2MGC148079
  • proline dehydrogenase (oxidase) 1
  • proline dehydrogenase (proline oxidase )
  • Proline oxidase
  • proline oxidase, mitochondrial
  • SCZD4
  • tumor protein p53 inducible protein 6

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PRODH Antibody (NB100-2832)(1)

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Bioinformatics

Gene Symbol PRODH
Entrez
Uniprot