Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody (OTI1B6)

Western Blot: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody (OTI1B6) [NBP2-02483] - Analysis of extracts (10ug) from 2 different cell lines by using anti-ALDH3A1 monoclonal antibody at 1:200 dilution.

Immunocytochemistry/Immunofluorescence: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody (OTI1B6) [NBP2-02483] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ALDH3A1.

Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody (OTI1B6) [NBP2-02483] - Staining of paraffin-embedded prostate tissue using anti-ALDH3A1 mouse monoclonal antibody.

Flow Cytometry: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody (OTI1B6) [NBP2-02483] - HEK293T cells transfected with either pCMV6-ENTRY ALDH3A1.(Red) or empty vector control plasmid (Blue) were immunostained with anti-ALDH3A1 mouse monoclonal, and then analyzed by flow cytometry.

Western Blot: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody (OTI1B6) [NBP2-02483] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALDH3A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALDH3A1.

Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody (OTI1B6) [NBP2-02483] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-ALDH3A1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody (OTI1B6) [NBP2-02483] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-ALDH3A1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody (OTI1B6) [NBP2-02483] - Staining of paraffin-embedded liver tissue using anti-ALDH3A1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody (OTI1B6) [NBP2-02483] - Staining of paraffin-embedded pancreas tissue using anti-ALDH3A1 mouse monoclonal antibody.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI1B6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody (OTI1B6) Summary

• Immunogen: Full length human recombinant protein of human ALDH3A1(NP_000682) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALDH3A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:2000
• Theoretical MW: 50.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody (OTI1B6)

• aldehyde dehydrogenase 3 family, member A1
• Aldehyde dehydrogenase 3
• Aldehyde Dehydrogenase 3A1
• Aldehyde Dehydrogenase 3-A1
• Aldehyde dehydrogenase family 3 member A1
• aldehyde dehydrogenase isozyme 3
• aldehyde dehydrogenase type III
• ALDH3A1
• ALDH3aldehyde dehydrogenase, dimeric NADP-preferring
• ALDHIII
• EC 1.2.1
• EC 1.2.1.5
• MGC10406
• stomach aldehyde dehydrogenase

Background

Aldehyde dehydrogenases oxidize various aldehydes to the corresponding acids. They are involved in the detoxification of alcohol-derived acetaldehyde and in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. The enzyme encoded by this gene forms a cytoplasmic homodimer that preferentially oxidizes aromatic and medium-chain (6 carbons or more) saturated and unsaturated aldehyde substrates. It is thought to promote resistance to UV and 4-hydroxy-2-nonenal-induced oxidative damage in the cornea. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ALDH3A1
• Entrez:
  • 218(Human)