ALDH2 Antibody (4G6A3)

Western Blot: ALDH2 Antibody (4G6A3) [NBP2-37397] - Western blot analysis using ALDH2 mouse mAb against HepG2 (1), A549 (2) cell lysate, and Rat live (3), Mouse liver (4), Mouse brain (5) tissue lysate.

Immunocytochemistry/Immunofluorescence: ALDH2 Antibody (4G6A3) [NBP2-37397] - Immunofluorescence analysis of HepG2 cells using ALDH2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

Immunohistochemistry: ALDH2 Antibody (4G6A3) [NBP2-37397] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ALDH2 mouse mAb with DAB staining.

Flow Cytometry: ALDH2 Antibody (4G6A3) [NBP2-37397] - Flow cytometric analysis of HeLa cells using ALDH2 mouse mAb (green) and negative control (red).

Western Blot: ALDH2 Antibody (4G6A3) [NBP2-37397] - Western blot analysis using ALDH2 mAb against human ALDH2 recombinant protein. (Expected MW is 47.4 kDa)

Western Blot: ALDH2 Antibody (4G6A3) [NBP2-37397] - Western blot analysis using ALDH2 mAb against HEK293 (1) and ALDH2 (AA: 317-517)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemistry: ALDH2 Antibody (4G6A3) [NBP2-37397] - Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ALDH2 mouse mAb with DAB staining.

ELISA: ALDH2 Antibody (4G6A3) [NBP2-37397] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
• Clone: 4G6A3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

ALDH2 Antibody (4G6A3) Summary

• Immunogen: Purified recombinant fragment of human ALDH2 (AA: 317-517) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALDH2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/Immunofluorescence 1:75
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • CyTOF-ready
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 56.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 0.5 % protein stabilizer.
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for ALDH2 Antibody (4G6A3)

• acetaldehyde dehydrogenase 2
• aldehyde dehydrogenase 2 family (mitochondrial)
• aldehyde dehydrogenase, mitochondrial
• ALDH class 2
• ALDH2
• ALDH-E2
• ALDHI
• ALDM
• EC 1.2.1
• EC 1.2.1.3
• liver mitochondrial ALDH
• MGC1806
• nucleus-encoded mitochondrial aldehyde dehydrogenase 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ALDH2