ALDH2 Antibody (4G6A3)

Images

 
Western Blot: ALDH2 Antibody (4G6A3) [NBP2-37397] - Western blot analysis using ALDH2 mouse mAb against HepG2 (1), A549 (2) cell lysate, and Rat live (3), Mouse liver (4), Mouse brain (5) tissue lysate.
Immunocytochemistry/ Immunofluorescence: ALDH2 Antibody (4G6A3) [NBP2-37397] - Immunofluorescence analysis of HepG2 cells using ALDH2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry: ALDH2 Antibody (4G6A3) [NBP2-37397] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ALDH2 mouse mAb with DAB staining.
Flow Cytometry: ALDH2 Antibody (4G6A3) [NBP2-37397] - Flow cytometric analysis of HeLa cells using ALDH2 mouse mAb (green) and negative control (red).
Western Blot: ALDH2 Antibody (4G6A3) [NBP2-37397] - Western blot analysis using ALDH2 mAb against human ALDH2 recombinant protein. (Expected MW is 47.4 kDa)
Western Blot: ALDH2 Antibody (4G6A3) [NBP2-37397] - Western blot analysis using ALDH2 mAb against HEK293 (1) and ALDH2 (AA: 317-517)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry: ALDH2 Antibody (4G6A3) [NBP2-37397] - Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ALDH2 mouse mAb with DAB staining.
ELISA: ALDH2 Antibody (4G6A3) [NBP2-37397] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
Clone
4G6A3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

ALDH2 Antibody (4G6A3) Summary

Immunogen
Purified recombinant fragment of human ALDH2 (AA: 317-517) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ALDH2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/Immunofluorescence 1:75
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
Application Notes
This antibody is Cytof ready.
Theoretical MW
56.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP2-37397.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 0.5 % protein stabilizer.
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for ALDH2 Antibody (4G6A3)

  • acetaldehyde dehydrogenase 2
  • aldehyde dehydrogenase 2 family (mitochondrial)
  • aldehyde dehydrogenase, mitochondrial
  • ALDH class 2
  • ALDH2
  • ALDH-E2
  • ALDHI
  • ALDM
  • EC 1.2.1
  • EC 1.2.1.3
  • liver mitochondrial ALDH
  • MGC1806
  • nucleus-encoded mitochondrial aldehyde dehydrogenase 2

Background

ALDH2 belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ALDH2 Antibody (NBP2-37397)(1)

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ALDH2