Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [Unconjugated]


Western blot shows lysates of human liver tissue, human testis tissue, and mouse liver. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse Aldehyde Dehydrogenase 1‑A1/ALDH1A1 Antigen Affinity-purified more
Aldehyde Dehydrogenase 1-A1/ALDH1A1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antigen Affinity-purified Polyclonal more
Aldehyde Dehydrogenase 1‑A1/ALDH1A1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Goat Anti-Human/Mouse Aldehyde Dehydrogenase 1‑A1/ALDH1A1 Antigen more
Simple Western lane view shows lysates of human liver tissue and mouse liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Aldehyde Dehydrogenase 1‑A1/ALDH1A1 at approximately 57 kDa (as indicated) more

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, IHC, IP, ICC/IF

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Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [Unconjugated] Summary

E. coli-derived recombinant human Aldehyde Dehydrogenase 1-A1/ALDH1A1
Accession # P00352
Detects human Aldehyde Dehydrogenase 1‑A1/ALDH1A1 in direct ELISAs and detects human and mouse Aldehyde Dehydrogenase 1‑A1/ALDH1A1 in Western blots.
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Read Publications using
AF5869 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [Unconjugated]

  • acetaldehyde dehydrogenase 1
  • ALDC
  • aldehyde dehydrogenase 1 family, member A1
  • aldehyde dehydrogenase 1, soluble
  • Aldehyde Dehydrogenase 1A1
  • Aldehyde Dehydrogenase 1-A1
  • Aldehyde dehydrogenase family 1 member A1
  • Aldehyde dehydrogenase, cytosolic
  • aldehyde dehydrogenase, liver cytosolic
  • ALDH class 1
  • ALDH1
  • ALDH11
  • ALDH1A1
  • ALDH-E1
  • EC 1.2.1
  • EC
  • MGC2318
  • PUMB1
  • RALDH 1
  • RALDH1
  • retinal dehydrogenase 1
  • retinaldehyde dehydrogenase 1


Aldehyde dehydrogenases (ALDHs) are NAD(P)+-dependent enzymes that detoxify aldehydes by oxidizing them to carboxylic acids. Nineteen ALDHs are present in humans, expressed in a variety of organelles and having different substrate preferences (1). ALDH1A1 is a cytosolic enzyme that preferentially oxidizes retinaldehyde to retinoic acid (2). ALDH1A1 is expressed in the epithelium of many organs, including brain, liver, testis, eye lens and cornea (3). ALDH1A1 is highly expressed in brain dopaminergic neurons, where it produces the retinoic acid required for their differentiation and development (4). The retinoic acid produced by ALDH1A1 is also important for the differentiation of hematopoietic stem cells (5). ALDH1A1 is a major enzyme in the oxidation of acetaldehyde, a toxic metabolite of ethanol (6).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (AF5869)(7)

We have publications tested in 3 confirmed species: Human, Mouse, Xenograft.

We have publications tested in 4 applications: Flow Cytometry, ICC, IHC, Western Blot.

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Showing Publications 1 - 7 of 7.
Publications using AF5869 Applications Species
X Liu, Y Ye, L Zhu, X Xiao, B Zhou, Y Gu, H Si, H Liang, M Liu, J Li, Q Jiang, J Li, S Yu, R Ma, S Su, JY Liao, Q Zhao Niche stiffness sustains cancer stemness via TAZ and NANOG phase separation Nature Communications, 2023-01-16;14(1):238. 2023-01-16 [PMID: 36646707] (IHC, Human) IHC Human
W Zeng, L Xiong, W Wu, S Li, J Liu, L Yang, L Lao, P Huang, M Zhang, H Chen, N Miao, Z Lin, Z Liu, X Yang, J Wang, P Wang, E Song, Y Yao, Y Nie, J Chen, D Huang CCL18 signaling from tumor-associated macrophages activates fibroblasts to adopt a chemoresistance-inducing phenotype Oncogene, 2022-11-22;0(0):. 2022-11-22 [PMID: 36418470] (Flow Cytometry, Xenograft) Flow Cytometry Xenograft
J Kim, J Jeon, B Song, N Lee, S Ko, Y Cha, P Leblanc, H Seo, KS Kim Spotting-based differentiation of functional dopaminergic progenitors from human pluripotent stem cells Nature Protocols, 2022-02-09;0(0):. 2022-02-09 [PMID: 35140411] (ICC, Human) ICC Human
M Pereira Lu, M Azcorra, G Caronia-Br, JF Poulin, Z Gaertner, S Gatica, OA Moreno-Ram, N Nouri, M Dubois, YC Ma, C Ramakrishn, L Fenno, YS Kim, K Deisseroth, F Cicchetti, DA Dombeck, R Awatramani Sox6 expression distinguishes dorsally and ventrally biased dopamine neurons in the substantia nigra with distinctive properties and embryonic origins Cell Reports, 2021-11-09;37(6):109975. 2021-11-09 [PMID: 34758317] (IHC, Mouse) IHC Mouse
J Wu, J Kung, J Dong, L Chang, C Xie, A Habib, S Hawes, N Yang, V Chen, Z Liu, R Evans, B Liang, L Sun, J Ding, J Yu, S Saez-Atien, B Tang, Z Khaliq, DT Lin, W Le, H Cai Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning Cell Rep, 2019-07-30;28(5):1167-1181.e7. 2019-07-30 [PMID: 31365862] (IHC, Mouse) IHC Mouse
C Sgobio, J Wu, W Zheng, X Chen, J Pan, AG Salinas, MI Davis, DM Lovinger, H Cai Aldehyde dehydrogenase 1-positive nigrostriatal dopaminergic fibers exhibit distinct projection pattern and dopamine release dynamics at mouse dorsal striatum Sci Rep, 2017-07-13;7(1):5283. 2017-07-13 [PMID: 28706191] (IHC, Mouse) IHC Mouse
W Cai, G Chen, Q Luo, J Liu, X Guo, T Zhang, F Ma, L Yuan, B Li, J Cai PMP22 Regulates Self-Renewal and Chemoresistance of Gastric Cancer Cells Mol. Cancer Ther, 2017-03-23;0(0):. 2017-03-23 [PMID: 28336807] (Western Blot, Human) Western Blot Human

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Gene Symbol ALDH1A1