PYCR1 Antibody

Western Blot: PYCR1 Antibody [H00005831-B01P] - Pig liver extracts. Lane 1: 30 ug, Lane 2: 45 ug, Lane 3: 60 ug, Lane 4: secondary anti-mouse antibody only. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: PYCR1 Antibody [H00005831-B01P] - Analysis of purified antibody to PYCR1 on HeLa cell (antibody concentration 10 ug/mL).

Western Blot: PYCR1 Antibody [H00005831-B01P] - Expression in U-2 OS.

Western Blot: PYCR1 Antibody [H00005831-B01P] - Analysis of PYCR1 expression in transfected 293T cell line by PYCR1 polyclonal antibody. Lane 1: PYCR1 transfected lysate (35.09 kDa). Lane 2: Non-transfected lysate.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Mouse
• Conjugate: Unconjugated

PYCR1 Antibody Summary

• Immunogen: PYCR1 (NP_008838.2, 1 a.a. - 319 a.a.) full-length human protein. MSVGFIGAGQLAFALAKGFTAAGVLAAHKIMASSPDMDLATVSALRKMGVKLTPHNKETVQHSDVLFLAVKPHIIPFILDEIGADIEDRHIVVSCAAGVTISSIEKKLSAFRPAPRVIRCMTNTPVVVREGATVYATGTHAQVEDGRLMEQLLSSVGFCTEVEEDLIDAVTGLSGSGPAYAFTALDALADGGVKMGLPRRLAVRLGAQALLGAAKMLLHSEQHPGQLKDNVSSPGGATIHALHVLESGGFRSLLINAVEASCIRTRELQSMADQEQVSPAAIKKTILDKVKLDSPAGTALSPSGHTKLLPRSLAPAGKD
• Specificity: PYCR1 - pyrroline-5-carboxylate reductase 1,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: PYCR1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control. It has been used for IF. Use in porcine reported by customer review.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

Quality control test: Antibody reactive against mammalian transfected lysate.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PYCR1 Antibody

• ARCL2B
• EC 1.5.1.2
• mitochondrial pyrroline-5-carboxylate reductase 1
• P5C reductase 1
• P5C
• P5CR 1
• P5CR
• PIG45
• PP222
• PRO3
• proliferation-inducing protein 45
• PYCR
• pyrroline-5-carboxylate reductase 1
• pyrroline-5-carboxylate reductase 1, mitochondrial

Background

This gene encodes an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. The protein forms a homopolymer and localizes to the mitochondrion. Alternate splicing results in two transcript variants encoding different isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PYCR1 Antibody (H00005831-B01P)(1)

Publication using H00005831-B01P

• De Ingeniis J, Ratnikov B, Richardson AD et al. Functional specialization in proline biosynthesis of melanoma. PLoS One. 2012-01-01 [PMID: 23024808] (Human)

Review for PYCR1 Antibody (H00005831-B01P) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Pig tissue.

reviewed by: Kwangwon son WB Pig tissue 04/22/2019

Summary

• Application: Western Blot
• Sample Tested: Guinea Pig Liver Extract,Pig liver extract
• Species: Pig tissue
• Lot: J4091

Comments

• Comments: * Primary Antibody dilution- 1:500 (overnight in 4°C)
  * Secondary Antibody dilution - 1:7,500 (anti-mouse)
  * 5% Blocking Solution
  * 125 mM NaCl in TBS-t
  * Exposure Time – 2min10sec with Pento ECL
  * Expected Molecular Weight – 35.09 KDa

Bioinformatics

• Gene Symbol: PYCR1
• Entrez:
  • 5831(Human)
• Uniprot:
  • NP_008838.2(Human)