PYCR1 Antibody

Images

 
Western Blot: PYCR1 Antibody [H00005831-B01P] - Pig liver extracts. Lane 1: 30 ug, Lane 2: 45 ug, Lane 3: 60 ug, Lane 4: secondary anti-mouse antibody only. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: PYCR1 Antibody [H00005831-B01P] - Analysis of purified antibody to PYCR1 on HeLa cell (antibody concentration 10 ug/mL).
Western Blot: PYCR1 Antibody [H00005831-B01P] - Expression in U-2 OS.
Western Blot: PYCR1 Antibody [H00005831-B01P] - Analysis of PYCR1 expression in transfected 293T cell line by PYCR1 polyclonal antibody. Lane 1: PYCR1 transfected lysate (35.09 kDa). Lane 2: Non-transfected lysate.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

PYCR1 Antibody Summary

Description
Quality control test: Antibody reactive against mammalian transfected lysate.
Immunogen
PYCR1 (NP_008838.2, 1 a.a. - 319 a.a.) full-length human protein. MSVGFIGAGQLAFALAKGFTAAGVLAAHKIMASSPDMDLATVSALRKMGVKLTPHNKETVQHSDVLFLAVKPHIIPFILDEIGADIEDRHIVVSCAAGVTISSIEKKLSAFRPAPRVIRCMTNTPVVVREGATVYATGTHAQVEDGRLMEQLLSSVGFCTEVEEDLIDAVTGLSGSGPAYAFTALDALADGGVKMGLPRRLAVRLGAQALLGAAKMLLHSEQHPGQLKDNVSSPGGATIHALHVLESGGFRSLLINAVEASCIRTRELQSMADQEQVSPAAIKKTILDKVKLDSPAGTALSPSGHTKLLPRSLAPAGKD
Specificity
PYCR1 - pyrroline-5-carboxylate reductase 1,
Isotype
IgG
Clonality
Polyclonal
Host
Mouse
Gene
PYCR1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control. It has been used for IF. Use in porcine reported by customer review.
Reviewed Applications
Read 1 Review rated 4
using
H00005831-B01P in the following applications:

Publications
Read Publication using H00005831-B01P.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PYCR1 Antibody

  • ARCL2B
  • EC 1.5.1.2
  • mitochondrial pyrroline-5-carboxylate reductase 1
  • P5C reductase 1
  • P5C
  • P5CR 1
  • P5CR
  • PIG45
  • PP222
  • PRO3
  • proliferation-inducing protein 45
  • PYCR
  • pyrroline-5-carboxylate reductase 1
  • pyrroline-5-carboxylate reductase 1, mitochondrial

Background

This gene encodes an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. The protein forms a homopolymer and localizes to the mitochondrion. Alternate splicing results in two transcript variants encoding different isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PYCR1 Antibody (H00005831-B01P)(1)

We have publications tested in 1 confirmed species: Human.


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(1)
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Review for PYCR1 Antibody (H00005831-B01P) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Pig tissue.

Reviews using H00005831-B01P:
Filter by Applications
WB
(1)
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Pig tissue
(1)
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Images Ratings Applications Species Date Details
Western Blot PYCR1 H00005831-B01P
Enlarge
4
reviewed by:
Verified Customer
WB Pig tissue 04/22/2019
View

Summary

ApplicationWestern Blot
Sample TestedGuinea Pig Liver Extract,Pig liver extract
SpeciesPig tissue
LotJ4091

Comments

Comments* Primary Antibody dilution- 1:500 (overnight in 4°C)
* Secondary Antibody dilution - 1:7,500 (anti-mouse)
* 5% Blocking Solution
* 125 mM NaCl in TBS-t
* Exposure Time – 2min10sec with Pento ECL
* Expected Molecular Weight – 35.09 KDa

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PYCR1 Antibody (H00005831-B01P) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
mFluor Violet Conjugated Antibodies

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Recent Reviews

4
5
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4
1
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2
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1
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Verified Customer
04/22/2019
Application: WB
Species: Pig tissue

Bioinformatics

Gene Symbol PYCR1
Entrez
Uniprot