Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody

Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NB100-787] - Staining of human (A), mouse (B) and rat (C) liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NB100-787] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody Summary

• Immunogen: Peptide with sequence C-EVKTVTVKISQKNS corresponding to C-Terminus according to NP_000680.2.
• Marker: Astrocyte Marker
• Specificity: This antibody may cross-react with ALDH1A2 (GeneID 8854) with one residue difference from this design, and with ALDH2 (GeneID 217) with two residues difference from this design.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ALDH1A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Immunohistochemistry
  • Peptide ELISA detection limit 1:1000
  • Western Blot 0.1-0.3 ug/ml
• Application Notes: WB: Approx 55 kDa band observed in human, mouse and rat liver lysates (calculated MW of 54.9 kDa according to NP_000680.2). IHC: successful usage on mouse Peyer's patches is reported in scientific literature (PMID: 15485630).
• Control:

  293 Whole Cell Lysate
  Mouse Spleen Whole Tissue Lysate (Adult Whole Normal)
  Aldehyde Dehydrogenase 1-A1/ALDH1A1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody

• acetaldehyde dehydrogenase 1
• ALDC
• aldehyde dehydrogenase 1 family, member A1
• aldehyde dehydrogenase 1, soluble
• Aldehyde Dehydrogenase 1A1
• Aldehyde Dehydrogenase 1-A1
• Aldehyde dehydrogenase family 1 member A1
• Aldehyde dehydrogenase, cytosolic
• aldehyde dehydrogenase, liver cytosolic
• ALDH class 1
• ALDH1
• ALDH11
• ALDH1A1
• ALDH-E1
• ALHDII
• EC 1.2.1
• EC 1.2.1.36
• MGC2318
• PUMB1
• RALDH 1
• RALDH1
• retinal dehydrogenase 1
• retinaldehyde dehydrogenase 1

Background

A protease inhibitor that inhibits fibrin clot formation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (NB100-787)(5)

Publications using NB100-787

• Kim D, Ahn J, Suh Y et al. Retinol Binding Protein 7 Promotes Adipogenesis in vitro and Regulates Expression of Genes Involved in Retinol Metabolism Frontiers in Cell and Developmental Biology [PMID: 35493071] (WB, Mouse)
• Eminel S, Jin N, Rostami M et al. Dimethyl- and monomethylfumarate regulate indolamine 2,3-dioxygenase (IDO) activity in human immune cells Exp. Dermatol. 2016-07-04 [PMID: 27376248] (WB, Mouse)
• Fishman-Jacob T, Reznichenko L, Youdim MB, et al. A sporadic Parkinson disease model via silencing of the ubiquitin-proteasome/E3 ligase component SKP1A J Biol Chem 2009-11-01 [PMID: 19748892]
• Iwata M, Hirakiyama A, Eshima Y et al. Retinoic acid imprints gut-homing specificity on T cells. Immunity 2004-10-01 [PMID: 15485630]
• Zheng CF, Wang TT, Weiner H. Cloning and expression of the full-length cDNAS encoding human liver class 1 and class 2 aldehyde dehydrogenase. Alcohol Clin Exp Res 1993-08-01 [PMID: 8214422]

Bioinformatics

• Gene Symbol: ALDH1A1
• Entrez:
  • 216(Human)
  • 11668(Mouse)
  • 24188(Rat)
• Uniprot:
  • NP_000680(Human)