Aldehyde Dehydrogenase 1-A1/ALDH1A1: Novus Biologicals

AF5869

Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [Unconjugated]

100 μg

AF5869

HAF019). A specific band was detected for Aldehyde Dehydrogenase 1-A1/ALDH1A1 at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_lightbox">

Western blot shows lysates of human liver tissue, human testis tissue, and mouse liver. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5869) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF019). A specific band was detected for Aldehyde Dehydrogenase 1-A1/ALDH1A1 at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." alt="Western blot shows lysates of human liver tissue, human testis tissue, and mouse liver. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5869) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Aldehyde Dehydrogenase 1-A1/ALDH1A1 at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_thumb" />

042-206). This experiment was conducted under reducing conditions and using the 12-230kDa separation system." class="big_lightbox">

Simple Western lane view shows lysates of Exosome Standards (HT‑29) (NBP3-11685), Exosome Standards (Human Urine) (NBP2-49840) and HepG2 human hepatocellular carcinoma cell line, loaded at 0.5 mg/ml. A specific band was detected for Aldehyde Dehydrogenase 1‑A1/ALDH1A1 at approximately 56 kDa (as indicated) using 10 µg/ml of Goat Anti-Human/Mouse Aldehyde Dehydrogenase 1‑A1/ALDH1A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5869) followed by HRP-conjugated Donkey Anti-Goat Secondary Antibody (Catalog # <a class=NoLineLink href=

042-206). This experiment was conducted under reducing conditions and using the 12-230kDa separation system." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

Aldehyde Dehydrogenase 1-A1/ALDH1A1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5869) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

Aldehyde Dehydrogenase 1-A1/ALDH1A1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Goat Anti-Human/Mouse Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5869) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # <a class=

CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

Simple Western lane view shows lysates of human liver tissue and mouse liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Aldehyde Dehydrogenase 1-A1/ALDH1A1 at approximately 57 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5869) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , ,

Applications: , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF5869U-100UG
Alexa Fluor 405	AF5869V-100UG
Alexa Fluor 488	AF5869G-100UG
Alexa Fluor 532	AF5869X-100UG
Alexa Fluor 594	AF5869T-100UG
Alexa Fluor 647	AF5869R-100UG
Alexa Fluor 700	AF5869N-100UG
Alexa Fluor 750	AF5869S-100UG
Alexa Fluor Plus 405	AF5869AFP405-100UG
Alexa Fluor Plus 488	AF5869AFP488-100UG
Alexa Fluor Plus 555	AF5869AFP555-100UG
Alexa Fluor Plus 594	AF5869AFP594-100UG
Alexa Fluor Plus 647	AF5869AFP647-100UG
Alexa Fluor Plus 680	AF5869AFP680-100UG
Allophycocyanin	AF5869A
Biotin	AF5869B
CoraFluor 1	AF5869CL1
DyLight 350	AF5869D
DyLight 405	AF5869E
DyLight 488	AF5869K
DyLight 550	AF5869L
DyLight 594	AF5869M
DyLight 650	AF5869W
DyLight 680	AF5869Y
DyLight 755	AF5869Z
FITC	AF5869F
HRP	AF5869H
Janelia Fluor 525	AF5869JF525
Janelia Fluor 549	AF5869I
Janelia Fluor 646	AF5869J
Janelia Fluor 669	AF5869JF669
PE	AF5869P
PerCP	AF5869C
mFluor Violet 450 SE	AF5869MFV450
mFluor Violet 500 SE	AF5869MFV500
mFluor Violet 610 SE	AF5869MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF5869-SP

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MAB5869

Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (703410) [Unconjugated]

(3 Publications)

100 μg

MAB5869

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

Aldehyde Dehydrogenase 1-A1/ALDH1A1 was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human Aldehyde Dehydrogenase 1-A1/ALDH1A1 Monoclonal Antibody (Catalog # MAB5869) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="Aldehyde Dehydrogenase 1-A1/ALDH1A1 was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human Aldehyde Dehydrogenase 1-A1/ALDH1A1 Monoclonal Antibody (Catalog # MAB5869) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

Aldehyde Dehydrogenase 1-A1/ALDH1A1 was detected in immersion fixed paraffin-embedded sections of human brain (substantia nigra) using Mouse Anti-Human Aldehyde Dehydrogenase 1-A1/ALDH1A1 Monoclonal Antibody (Catalog # MAB5869) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Aldehyde Dehydrogenase 1‑A1/ALDH1A1 at approximately 58 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Aldehyde Dehydrogenase 1‑A1/ALDH1A1 Monoclonal Antibody (Catalog # MAB5869). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

HAF007). A specific band was detected for Aldehyde Dehydrogenase 1-A1/ ALDH1A1 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of human liver tissue, mouse liver tissue, and rat lung tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Aldehyde Dehydrogenase 1-A1/ALDH1A1 Monoclonal Antibody (Catalog # MAB5869) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF007). A specific band was detected for Aldehyde Dehydrogenase 1-A1/ ALDH1A1 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HTRA2 overexpression inhibitsDCIS.com breast cancer cell growth and invasion. (A–F) DCIS.com cells expressing pHAGE control or HTRA2 were analyzed for (A) ALDH1A1 and HTRA2 expression by immunoblot, (B) spheroid size in 3D cultures, (C) PCNA expression, (D) transwell invasion, (E) E-cadherin or (F) TWIST1 expression. Mean number of spheroids analyzed per group±s.e.m.: pHAGE, 325±25; pHAGE±CCL2, 358±33; HTRA2-OE, 343±17; HTRA2-OE±CCL2, 316±44. Protein levels in immunoblots were measured by densitometry. Expression levels were normalized to control group. Representative blots of three experiments are shown. Spheroid size and invasion index were normalized to spheroid number. Experimental groups were plated in triplicate. Experiments were performed three times. Two slides containing three sections each were subject to immunostaining. Statistical analysis was performed using two-tailed t-test (A) or one-way ANOVA with Bonferroni post-hoc comparison (B–F). *P<0.05, **P<0.01, ***P<0.001; n.s, not significant. Mean±s.e.m. is shown. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31208996), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Genetic Strategies Validation. </b>HTRA2 overexpression orknockdown affects CCR2-H SUM225 and CCR2-KO DCIS.com breast cancer cell growth and invasion. (A–D) CCR2-H SUM225 cells expressing vehicle control (pHAGE) or HTRA2 (HTRA2-OE) were analyzed for HTRA2 expression by immunoblot (A), spheroid size in 3D cultures (B), PCNA expression (C) or invasion index (D). Mean number of spheroids analyzed per group±s.e.m.: pHAGE, 269±63; HTRA2-OE, 227±28. (E–H) CCR2-KO DCIS.com cells were transduced with lentivirus expressing Con or HTRA2 shRNAs (HTRA2-KD#2, HTRA2-KD#3) and analyzed for HTRA2 expression by immunoblot (E), spheroid size in Matrigel: Collagen (F), PCNA expression (G) or transwell invasion (H). Spheroid size and invasion index were normalized to spheroid number. Experimental groups were plated in triplicate. Experiments were repeated three times. Two slides containing three sections each were subject to immunostaining. Mean number of spheroids analyzed/group±s.e.m.: Con, 196±80; HTRA2-KD#2, 219±99; HTRA2-KD#2, 161±40. Statistical analysis was performed using two-tailed t-test (A–D) or one-way ANOVA with Bonferroni post-hoc comparison (E–H). *P<0.05, **P<0.01, ***P<0.001; n.s, not significant. Mean±s.e.m. is shown. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31208996), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Genetic Strategies Validation. </b>Effect of p42/44MAPK and SMAD3 knockdown on CCL2/CCR2-mediated ALDH1A1 and HTRA2 expression. (A) DCIS.com, pHAGE or CCR2-H SUM225 cell lines were analyzed for expression of the indicated proteins by immunoblot. Arrow points to SMAD3 protein approximately 50 kda in size. (B) DCIS.com or CCR2-H SUM225 cells were transfected with control siRNAs or siRNAs to p42/44MAPK or SMAD3, stimulated with CCL2 for 24 h and analyzed for expression of the indicated proteins by immunoblot. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31208996), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Genetic Strategies Validation. </b>ALDH1A1 knockdown inhibits DCIS.com breast cancer cell growth and invasion. (A–F) DCIS.com cells expressing control shRNAs (Con) or shRNAs to ALDH1A1 (ALDH-KD#1, ALDH-KD#5) were treated with/without 100 ng/ml CCL2 and analyzed for (A) ALDH1A1 and HTRA2 expression by immunoblot, (B) spheroid size in 3D cultures, (C) PCNA expression, (D) transwell invasion, (E) E-cadherin or (F) TWIST1 expression. Mean number of spheroids analyzed per group±s.e.m.: Con, 163+9; Con+CCL2, 165+7; ALDH-KD#1 152+12; ALDH-KD#1+CCL2, 168+11; ALDH-KD#5, 174+10; ALDH-KD#5+CCL2, 162+13. Protein levels in immunoblots were measured by densitometry. Expression levels were normalized to control group. Representative blots of three experiments are shown. Spheroid size and invasion index were normalized to spheroid number. Experimental groups were plated in triplicate. Experiments were performed three times. Two slides containing three sections each were subject to immunostaining. Statistical analysis was performed using one-way ANOVA with Bonferroni post-hoc comparison. *P<0.05, **P<0.01; n.s, not significant. Mean±s.e.m. is shown. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31208996), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Genetic Strategies Validation. </b>ALDH1A1 overexpression orknockdown affects CCR2-H SUM225 and CCR2-KO DCIS.com breast cancer cell growth but not invasion. (A–D) CCR2-H SUM225 cells expressing Con shRNAs or shRNAs to ALDH1A1 (ALDH-KD#1, ALDH-KD#5) were analyzed for ALDH1A1 and HTRA2 expression by immunoblot (A), spheroid size in 3D cultures (B), PCNA expression (C) or invasion index (D). Mean number of spheroids analyzed per group±s.e.m.: Con, 197±18; ALDH-KD#1, 190±10; ALDH-KD#5, 196±87. (E–H) CCR2-KO DCIS.com cells expressing vehicle control (pHAGE) or ALDH1A1 (ALDH-OE) were analyzed for: expression of ALDH1A1 and HTRA2 by immunoblot (E), spheroid size in 3D cultures (F), PCNA expression (G) or transwell invasion (H). Protein levels in immunoblots were measured by densitometry. Expression levels were normalized to control group. Representative blots of three experiments are shown. Spheroid size and invasion index were normalized to spheroid number. Experimental groups were plated in triplicate. Experiments were performed three times. Two slides containing three sections each were subject to immunostaining. Mean number of spheroids analyzed per group±s.e.m.: pHAGE, 275±58; ALDH-OE, 219±38. Statistical analysis was performed using one-way ANOVA with Bonferroni post-hoc comparison. *P<0.05; n.s, not significant. Mean±s.e.m. is shown. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31208996), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB5869U-100UG
Alexa Fluor 405	FAB5869V-100UG
Alexa Fluor 488	FAB5869G-100UG
Alexa Fluor 532	FAB5869X-100UG
Alexa Fluor 594	FAB5869T-100UG
Alexa Fluor 647	FAB5869R-100UG
Alexa Fluor 700	FAB5869N-100UG
Alexa Fluor 750	FAB5869S-100UG
Alexa Fluor Plus 405	FAB5869AFP405-100UG
Alexa Fluor Plus 488	FAB5869AFP488-100UG
Alexa Fluor Plus 555	FAB5869AFP555-100UG
Alexa Fluor Plus 594	FAB5869AFP594-100UG
Alexa Fluor Plus 647	FAB5869AFP647-100UG
Alexa Fluor Plus 680	FAB5869AFP680-100UG
Allophycocyanin	FAB5869A
Biotin	FAB5869B
CoraFluor 1	FAB5869CL1
DyLight 350	FAB5869D
DyLight 405	FAB5869E
DyLight 488	FAB5869K
DyLight 550	FAB5869L
DyLight 594	FAB5869M
DyLight 650	FAB5869W
DyLight 680	FAB5869Y
DyLight 755	FAB5869Z
FITC	FAB5869F
HRP	FAB5869H
Janelia Fluor 525	FAB5869JF525
Janelia Fluor 549	FAB5869I
Janelia Fluor 646	FAB5869J
Janelia Fluor 669	FAB5869JF669
PE	FAB5869P
PerCP	FAB5869C
mFluor Violet 450 SE	FAB5869MFV450
mFluor Violet 500 SE	FAB5869MFV500
mFluor Violet 610 SE	FAB5869MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB5869-SP

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NBP2-15336

Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody

(3 Publications)

0.1 ml

NBP2-15336

Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15336] - Detects ALDH1A1 protein by Western blot analysis. A. 50 ug rat liver lysate/extract 10 % SDS-PAGE Dilution: 1:5000

Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15336] - Detects ALDH1A1 protein by Western blot analysis. A. 50 ug mouse liver lysate/extract 10 % SDS-PAGE Dilution: 1:5000

Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15336] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A1 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15336] - Human gastric cancer, using ALDH1A1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

ALDH1A1 antibody detects Aldh1a2 protein on whole-mount zebrafish embryos by immunohistochemical analysis.?<br /> Sample: Paraformaldehyde-fixed zebrafish embryos. <br /> ALDH1A1 antibody (NBP2-15336) dilution: 1:200.

Reactivity:

Hu, Mu, Rt, Fe, Ze, Bv, Ca, Pm, Rb, Sh

Species Glossary

Reactivity: Hu, Mu, Rt, Fe, Ze, Bv, Ca, Pm, Rb, Sh

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP1-89152

Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody - BSA Free

(4 Publications)

0.1 ml

NBP1-89152

Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP1-89152] - Staining of human tonsil shows very weak positivity in non-germinal center cells.

Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP1-89152] - Staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP1-89152] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.

Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP1-89152] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-89152-25ul

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NB100-787

Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody

(5 Publications)

0.1 mg

NB100-787

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Unconjugated

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NBP2-15334

Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody

0.1 ml

NBP2-15334

Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15334] - Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE; antibody diluted at 1:5000.

Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15334] - A. 50 ug mouse liver lysate/extract 10 % SDS-PAGE ALDH1A1 antibody dilution: 1:1000

Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15334] - PG-4 whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Aldehyde Dehydrogenase 1-A1/ALDH1A1 antibody (NBP2-15334) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

ALDH1A1 antibody detects ALDH1A1 protein by western blot analysis.<br />A. 50 ug rat liver lysate/extract<br />10% SDS-PAGE<br />ALDH1A1 antibody (NBP2-15334) dilution: 1:5000 <br />The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Reactivity:

Hu, Mu, Rt, Fe, Bv, Ca, Pm, Rb, Sh

Species Glossary

Reactivity: Hu, Mu, Rt, Fe, Bv, Ca, Pm, Rb, Sh

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NB100-2563

Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody

(2 Publications)

0.1 mg

NB100-2563

Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NB100-2563] - (1ug/ml) Analysis of Human Liver Lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Unconjugated

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H00000216-M05

Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (1G6) - Azide and BSA Free

(1 Publication)

0.1 mg

H00000216-M05

Immunoprecipitation: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (1G6) [H00000216-M05] - Analysis of ALDH1A1 transfected lysate using anti-ALDH1A1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ALDH1A1 rabbit polyclonal antibody.

ELISA: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (1G6) [H00000216-M05] - Detection limit for recombinant GST tagged ALDH1A1 is 1 ng/ml as a capture antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP2-53248

Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (ALDH1A1/1381)

100 μg

NBP2-53248

Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (ALDH1A1/1381) [NBP2-53248] - Western Blot of K562 and Lung lysate Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (ALDH1A1/1381).

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-54422AF350
Alexa Fluor 405	NBP2-54422AF405
Alexa Fluor 488	NBP2-54422AF488
Alexa Fluor 532	NBP2-54422AF532
Alexa Fluor 594	NBP2-54422AF594
Alexa Fluor 647	NBP2-54422AF647
Alexa Fluor 700	NBP2-54422AF700
Alexa Fluor 750	NBP2-54422AF750
Alexa Fluor Plus 405	NBP2-54422AFP405
Alexa Fluor Plus 488	NBP2-54422AFP488
Alexa Fluor Plus 555	NBP2-54422AFP555
Alexa Fluor Plus 594	NBP2-54422AFP594
Alexa Fluor Plus 647	NBP2-54422AFP647
Alexa Fluor Plus 680	NBP2-54422AFP680
Allophycocyanin	NBP2-54422APC
Biotin	NBP2-54422B
CoraFluor 1	NBP2-54422CL1
DyLight 350	NBP2-54422UV
DyLight 405	NBP2-54422V
DyLight 488	NBP2-54422G
DyLight 550	NBP2-54422R
DyLight 594	NBP2-54422DL594
DyLight 650	NBP2-54422C
DyLight 680	NBP2-54422FR
DyLight 755	NBP2-54422IR
FITC	NBP2-54422F
HRP	NBP2-54422H
Janelia Fluor 525	NBP2-54422JF525
Janelia Fluor 549	NBP2-54422JF549
Janelia Fluor 646	NBP2-54422JF646
Janelia Fluor 669	NBP2-54422JF669
PE	NBP2-54422PE
mFluor Violet 450 SE	NBP2-54422MFV450
mFluor Violet 500 SE	NBP2-54422MFV500
mFluor Violet 610 SE	NBP2-54422MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-54422-100ug

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NBP3-49099

Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (rALDH1A1/7285)

100 μg

NBP3-49099

Formalin-fixed, paraffin-embedded human lung stained with Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (rALDH1A1/7285). HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human lung stained with Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (rALDH1A1/7285). Inset: PBS instead of primary antibody; secondary only negative control.

Western Blot Analysis of Human Brain tissue lysate using Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (rALDH1A1/7285).

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP3-49634

Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (rALDH1A1/7285) - Azide and BSA Free

100 μg

NBP3-49634

Formalin-fixed, paraffin-embedded human lung stained with Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (rALDH1A1/7285) - Azide and BSA Free. Inset: PBS instead of primary antibody; secondary only negative control.

Western Blot Analysis of Human Brain tissue lysate using Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (rALDH1A1/7285) - Azide and BSA Free.

Formalin-fixed, paraffin-embedded human lung stained with Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (rALDH1A1/7285) - Azide and BSA Free. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP3-32019

Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (8F1-R)

100 ul

NBP3-32019

Western blot analysis on different lysates with NBP3-32019 at 1/1,000 dilution. Lane 1: A549 cell lysate (20 µg/Lane) Lane 2: HT-29 cell lysate (20 µg/Lane) Lane 3: HepG2 cell lysate (20 µg/Lane) Lane 4: K-562 cell lysate (20 µg/Lane) Lane 5: Human liver tissue lysate (40 µg/Lane) Lane 6: Human lung tissue lysate (40 µg/Lane) Predicted band size: 55 kDa Observed band size: 50 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32019 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat anti-Mouse IgG [HRP] at 1/50,000 dilution was used for 1 hour at room temperature.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

ALDH1A1 Products By Gene Name

Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [Unconjugated]

Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (703410) [Unconjugated]

Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody

Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody - BSA Free

Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody

Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody

Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody

Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (1G6) - Azide and BSA Free

Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (ALDH1A1/1381)

Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (rALDH1A1/7285)

Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (rALDH1A1/7285) - Azide and BSA Free

Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (8F1-R)