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Immunohistochemistry-Frozen: ALDH1A3 Antibody [NBP2-15339] - 8-week old mouse pancreas cryosections with beta cell dedifferentiation properties were stained with ALDH1A3 (1:500 dilution) and Insulin (1:1000 dilution) ...read more
Western Blot: ALDH1A3 Antibody [NBP2-15339] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], diluted at 1:1000. The HRP-conjugated anti-rabbit IgG ...read more
Immunohistochemistry: ALDH1A3 Antibody [NBP2-15339] - Markers of dedifferentiation are present in islets of db/db, but not weight-matched WD-fed mice. Staining for Aldh1a3 protein (red) and insulin (green) in islets ...read more
Western Blot: ALDH1A3 Antibody [NBP2-15339] - Total protein from human Lymph node, mouse Stomach and A431 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in ...read more
Immunocytochemistry/ Immunofluorescence: ALDH1A3 Antibody [NBP2-15339] - A431 cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated ...read more
Western Blot: ALDH1A3 Antibody [NBP2-15339] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], diluted at 1:2000.
Immunocytochemistry/ Immunofluorescence: ALDH1A3 Antibody [NBP2-15339] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ALDH1A3 stained by ALDH1A3 antibody [N2C2], diluted at 1:500. Blue: Hoechst ...read more
Immunocytochemistry/ Immunofluorescence: ALDH1A3 Antibody [NBP2-15339] - Human breast cancer cells with Aldh1a3 overexpression were stained at 1:500 in 5% goat serum in PBS-T. Human breast cancer cells were transduced ...read more
Immunohistochemistry-Paraffin: ALDH1A3 Antibody [NBP2-15339] - Mouse prostate. ALDH1A3 stained by ALDH1A3 antibody [N2C2], diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: ALDH1A3 Antibody [NBP2-15339] - Rat prostate. ALDH1A3 stained by ALDH1A3 antibody [N2C2], diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
IHC-Fr usage reported in scientific literature (PMID: 26713822) and a verfied customer reveiw. Use in Immunoblotting reported in scientific literature (PMID: 27572106). This ALDH1A3 antibody is validated for ICC/IF from a verified customer review.
Theoretical MW
56 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 3 Reviews rated 4.7 using NBP2-15339 in the following applications:
Use in Mouse reported in scientific literature (PMID:34572374). Mouse reactivity reported in scientific literature (PMID: 28249000). Chicken (88%)., Xenopus laevis (83%). .
Packaging, Storage & Formulations
Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified
Alternate Names for ALDH1A3 Antibody - BSA Free
aldehyde dehydrogenase 1 family, member A3
Aldehyde dehydrogenase 6
aldehyde dehydrogenase family 1 member A3
ALDH1A6
ALDH6acetaldehyde dehydrogenase 6
EC 1.2.1
EC 1.2.1.5
RALDH3
RALDH-3
Retinaldehyde dehydrogenase 3
Background
Aldehyde dehydrogenase isozymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. The enzyme encoded by this gene uses retinal as a substrate, either in a free or cellular retinol-binding protein form. [provided by RefSeq]
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Bioinformatics Tool for ALDH1A3 Antibody (NBP2-15339)
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Diseases for ALDH1A3 Antibody (NBP2-15339)
Discover more about diseases related to ALDH1A3 Antibody (NBP2-15339).
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