ALDH1A3 Antibody

Images

 
Western Blot: ALDH1A3 Antibody [NBP2-15339] - Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE diluted at 1:10000
Immunocytochemistry: ALDH1A3 Antibody [NBP2-15339] - Analysis of methanol-fixed A549, using antibody at 1:500 dilution.
Immunohistochemistry-Frozen: ALDH1A3 Antibody [NBP2-15339] - 8 Week old mouse pancreas cryosections with beta cell dedifferentiation properties were stained with ALDH1A3 (1:500 dilution) and Insulin (1:1000 dilution) ...read more
Immunohistochemistry-Paraffin: ALDH1A3 Antibody [NBP2-15339] - Paraffin-embedded A549 xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity Hu, Mu, Rt, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

ALDH1A3 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human ALDH1A3. The exact sequence is proprietary.
Localization
Cytoplasm
Predicted Species
Rat (95%), Rhesus Macaque (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ALDH1A3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:20000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:1000
Application Notes
IHC-Fr usage reported in scientific literature (PMID: 26713822).
Theoretical MW
56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-15339 in the following applications:

Publications
Read Publications using
NBP2-15339 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28249000). Chicken (88%), Xenopus laevis (83%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
1XPBS, 20% Glycerol (pH7).
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for ALDH1A3 Antibody

  • aldehyde dehydrogenase 1 family, member A3
  • Aldehyde dehydrogenase 6
  • aldehyde dehydrogenase family 1 member A3
  • ALDH1A6
  • ALDH6acetaldehyde dehydrogenase 6
  • EC 1.2.1
  • EC 1.2.1.5
  • RALDH3
  • RALDH-3
  • Retinaldehyde dehydrogenase 3

Background

Aldehyde dehydrogenase isozymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. The enzyme encoded by this gene uses retinal as a substrate, either in a free or cellular retinol-binding protein form. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ALDH1A3 Antibody (NBP2-15339)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: IB, ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
IB
(1)
ICC/IF
(2)
IHC
(1)
IHC-Fr
(1)
IHC-P
(1)
WB
(1)
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Human
(3)
Mouse
(3)
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Showing Publications 1 - 7 of 7.
Publications using NBP2-15339 Applications Species
Burke SJ, Batdorf HM, Burk DH et al. db/db Mice Exhibit Features of Human Type 2 Diabetes That Are Not Present in Weight-Matched C57BL/6J Mice Fed a Western Diet. J Diabetes Res. 2017 Oct 17 [PMID: 29038790] (ICC/IF, Mouse) ICC/IF Mouse
Ramzy A, Mojibian M, Kieffer TJ. Insulin deficient mouse b-cells do not fully mature but can be remedied through insulin replacement by islet transplantation. Endocrinology. 2017 Sep 27 [PMID: 29029025] (Mouse) Mouse
Li M, Xiao A, Floyd D et al. CDK4/6 inhibition is more active against the glioblastoma proneural subtype Oncotarget 2017 Aug 15 [PMID: 28903422] (WB, Human) WB Human
Feng AL, Xiang YY, Gui L et al. Paracrine GABA and insulin regulate pancreatic alpha cell proliferation in a mouse model of type 1 diabetes. Diabetologia. Mar 9 2017 [PMID: 28280900] (Mouse) Mouse
Kim-Muller Jy, Fan J, Kim Yj et al. Aldehyde dehydrogenase 1a3 defines a subset of failing pancreatic beta cells in diabetic mice. Nat Commun Aug 30 2016 [PMID: 27572106] (IHC, IB) IHC, IB
Cinti F, Bouchi R, Kim-Muller JY et al. Evidence of B-cell Dedifferentiation in Human Type 2 Diabetes. J. Clin. Endocrinol. Metab. 2015 Dec 29 [PMID: 26713822] (IHC-Fr, Human) IHC-Fr Human
Dahlhoff M, Frohlich T, Arnold GJ et al. Characterization of the sebocyte lipid droplet proteome reveals novel potential regulators of sebaceous lipogenesis. Exp. Cell Res. 2014 Dec 16 [PMID: 25523620] (ICC/IF, IHC-P, Human) ICC/IF, IHC-P Human

Review for ALDH1A3 Antibody (NBP2-15339) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: IHC-Fr.

Reviews using NBP2-15339:
Filter by Applications
IHC-Fr
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen ALDH1A3 NBP2-15339
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reviewed by:
Anonymous
IHC-Fr Mouse 11/09/2016
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedMouse Pancreas
SpeciesMouse

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ALDH1A3 Antibody (NBP2-15339) (0)

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Secondary Antibodies

 

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Recent Reviews

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1
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Anonymous
11/09/2016
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol ALDH1A3
Uniprot