ALDH1A3 Antibody

Immunohistochemistry-Frozen: ALDH1A3 Antibody [NBP2-15339] - 8-week old mouse pancreas cryosections with beta cell dedifferentiation properties were stained with ALDH1A3 (1:500 dilution) and Insulin (1:1000 dilution) antibodies. Blue arrows indicate insulin+Aldh1A3+ cells. IHC-Fr image submitted by a verified customer review.

Western Blot: ALDH1A3 Antibody [NBP2-15339] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry: ALDH1A3 Antibody [NBP2-15339] - Markers of dedifferentiation are present in islets of db/db, but not weight-matched WD-fed mice. Staining for Aldh1a3 protein (red) and insulin (green) in islets from weight-matched WD-fed mice (20 weeks on diet) versus db/db mice (14 weeks of age). Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/jdr/2017/8503754/) licensed under a CC-BY licence.

Western Blot: ALDH1A3 Antibody [NBP2-15339] - Total protein from human Lymph node, mouse Stomach and A431 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-ALDH1A3 (NBP2-15339) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Immunocytochemistry/Immunofluorescence: ALDH1A3 Antibody [NBP2-15339] - A431 cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-ALDH1A3 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Western Blot: ALDH1A3 Antibody [NBP2-15339] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], diluted at 1:2000.

Immunocytochemistry/Immunofluorescence: ALDH1A3 Antibody [NBP2-15339] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ALDH1A3 stained by ALDH1A3 antibody [N2C2], diluted at 1:500. Blue: Hoechst 33342 staining.

Immunocytochemistry/Immunofluorescence: ALDH1A3 Antibody [NBP2-15339] - Human breast cancer cells with Aldh1a3 overexpression were stained at 1:500 in 5% goat serum in PBS-T. Human breast cancer cells were transduced with human Aldh1a3 and probed with antibody at 1:500 overnight at 4C followed by counterstaining with secondary and DAPI. Strong signal observed compared to knockout controls. ICC/IF image submitted by a verified customer review.

Immunohistochemistry-Paraffin: ALDH1A3 Antibody [NBP2-15339] - Mouse prostate. ALDH1A3 stained by ALDH1A3 antibody [N2C2], diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: ALDH1A3 Antibody [NBP2-15339] - Rat prostate. ALDH1A3 stained by ALDH1A3 antibody [N2C2], diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

• Reactivity: Hu, Mu, Rt, RM
• Applications: WB, IB, ICC/IF, IHC, IHC-Fr, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

ALDH1A3 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human ALDH1A3. The exact sequence is proprietary.
• Localization: Cytoplasm
• Predicted Species: Rhesus Macaque (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ALDH1A3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:3000
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:100 - 1:1000
  • Immunohistochemistry 1:100 - 1:1000
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100 - 1:1000
• Application Notes: IHC-Fr usage reported in scientific literature (PMID: 26713822) and a verfied customer reveiw. Use in Immunoblotting reported in scientific literature (PMID: 27572106). This ALDH1A3 antibody is validated for ICC/IF from a verified customer review.
• Theoretical MW: 56 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28249000). Chicken (88%), Xenopus laevis (83%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ALDH1A3 Antibody

• aldehyde dehydrogenase 1 family, member A3
• Aldehyde dehydrogenase 6
• aldehyde dehydrogenase family 1 member A3
• ALDH1A6
• ALDH6acetaldehyde dehydrogenase 6
• EC 1.2.1
• EC 1.2.1.5
• RALDH3
• RALDH-3
• Retinaldehyde dehydrogenase 3

Background

Aldehyde dehydrogenase isozymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. The enzyme encoded by this gene uses retinal as a substrate, either in a free or cellular retinol-binding protein form. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ALDH1A3 Antibody (NBP2-15339)(17)

Publications using NBP2-15339

• Stephens CH, Morrison RA, McLaughlin M et al. Oligomeric collagen as an encapsulation material for islet/beta cell replacement: Effect of islet source, dose, implant site, and administration format Am. J. Physiol. Endocrinol. Metab. Jun 16 2020 [PMID: 32543944]
• Huang JL, Lee S, Hoek P et al. Genetic deletion of Urocortin 3 does not prevent functional maturation of beta cells J. Endocrinol. May 1 2020 [PMID: 32369775] (Mouse)
• Shrestha N, Liu T, Ji Y et Al. Sel1L-Hrd1 ER-associated degradation maintains beta-cell identity via TGF- beta signaling J. Clin. Invest. Mar 17 2020 [PMID: 32182217] (ICC/IF, WB, Mouse)
• Liu N, Cai X, Liu T et al. Hypoxia-inducible factor-1 alpha mediates the expression of mature beta cell-disallowed genes in hypoxia-induced beta cell dedifferentiation Biochem. Biophys. Res. Commun. Dec 19 2019 [PMID: 31866014] (IHC-P, Human)
• GOmez-Banoy N, Guseh JS, Li G et al. Adipsin preserves beta cells in diabetic mice and associates with protection from type 2 diabetes in humans Nat. Med. Nov 1 2019 [PMID: 31700183] (IHC, IHC-P, ICC/IF, Mouse)
• Rahman MT, Muppala S, Wu J et al. Induction of alpha cell-restricted Gc in dedifferentiating beta cells contributes to stress-induced beta-cell dysfunction JCI Insight May 23 2019 [PMID: 31120862] (IHC-P, Mouse)
• Tersey SA, Levasseur EM, Syed F et al. Episodic B-cell death and dedifferentiation during diet-induced obesity and dysglycemia in male mice FASEB J. May 29 2018 [PMID: 29812970] (Mouse)
• Wang NN, Wang LH, Li Y et al. Targeting ALDH2 with disulfiram/copper reverses the resistance of cancer cells to microtubule inhibitors Exp. Cell Res. 2017 Nov 16 [PMID: 29155365] (Mouse)
• Burke SJ, Batdorf HM, Burk DH et al. db/db Mice Exhibit Features of Human Type 2 Diabetes That Are Not Present in Weight-Matched C57BL/6J Mice Fed a Western Diet. J Diabetes Res. 2017 Oct 17 [PMID: 29038790] (ICC/IF, Mouse)
• Ramzy A, Mojibian M, Kieffer TJ. Insulin deficient mouse b-cells do not fully mature but can be remedied through insulin replacement by islet transplantation. Endocrinology. 2017 Sep 27 [PMID: 29029025] (Mouse)
• Li M, Xiao A, Floyd D et al. CDK4/6 inhibition is more active against the glioblastoma proneural subtype Oncotarget 2017 Aug 15 [PMID: 28903422] (WB, Human)
• Ishida E, kim-Muller JY, Accili D. Pair Feeding, but Not Insulin, Phloridzin, or Rosiglitazone Treatment, Curtails Markers of B-Cell Dedifferentiation in db/db Mice. Diabetes. Aug 1 2017 [PMID: 28506962] (ICC/IF, Mouse)
• Feng AL, Xiang YY, Gui L et al. Paracrine GABA and insulin regulate pancreatic alpha cell proliferation in a mouse model of type 1 diabetes. Diabetologia. Mar 9 2017 [PMID: 28280900] (Mouse)
• Kim-Muller Jy, Fan J, Kim Yj et al. Aldehyde dehydrogenase 1a3 defines a subset of failing pancreatic beta cells in diabetic mice. Nat Commun Aug 30 2016 [PMID: 27572106] (IHC, IB)
• Cinti F, Bouchi R, Kim-Muller JY et al. Evidence of B-cell Dedifferentiation in Human Type 2 Diabetes. J. Clin. Endocrinol. Metab. 2015 Dec 29 [PMID: 26713822] (IHC-Fr, Human)
• Dahlhoff M, Frohlich T, Arnold GJ et al. Characterization of the sebocyte lipid droplet proteome reveals novel potential regulators of sebaceous lipogenesis. Exp. Cell Res. 2014 Dec 16 [PMID: 25523620] (ICC/IF, IHC-P, Human)
• Fan J, Du W, Kim-Muller JY et al. Cyb5r3 links FoxO1-dependent mitochondrial dysfunction with beta-cell failure bioRxiv Sep 19 2019

Reviews for ALDH1A3 Antibody (NBP2-15339) (3)

Average Rating: 4.7

(Based on 3 reviews)

We have 3 reviews tested in 2 species: Human, Mouse.

reviewed by: Anonymous WB Human 03/08/2020

Summary

• Application: Western Blot
• Sample Tested: T47D human breast cancer cell line
• Species: Human
• Lot: 43082

reviewed by: Anonymous ICC Human 01/14/2020

Summary

• Application: Immunocytochemistry
• Sample Tested: Human breast cancer cells
• Species: Human
• Lot: 43082

Comments

• Comments: Human breast cancer cells with Aldh1a3 overexpression were stained at 1:500 in 5% goat serum in PBS-T.

reviewed by: Anonymous IHC-Fr Mouse 11/09/2016

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Mouse Pancreas
• Species: Mouse

Bioinformatics

• Gene Symbol: ALDH1A3
• Uniprot:
  • P47895(Human)