ALDH1A3 Antibody

Images

 
Immunohistochemistry-Frozen: ALDH1A3 Antibody [NBP2-15339] - 8-week old mouse pancreas cryosections with beta cell dedifferentiation properties were stained with ALDH1A3 (1:500 dilution) and Insulin (1:1000 dilution) ...read more
Western Blot: ALDH1A3 Antibody [NBP2-15339] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], diluted at 1:1000. The HRP-conjugated anti-rabbit IgG ...read more
Immunohistochemistry: ALDH1A3 Antibody [NBP2-15339] - Markers of dedifferentiation are present in islets of db/db, but not weight-matched WD-fed mice. Staining for Aldh1a3 protein (red) and insulin (green) in islets ...read more
Western Blot: ALDH1A3 Antibody [NBP2-15339] - Total protein from human Lymph node, mouse Stomach and A431 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in ...read more
Immunocytochemistry/ Immunofluorescence: ALDH1A3 Antibody [NBP2-15339] - A431 cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated ...read more
Western Blot: ALDH1A3 Antibody [NBP2-15339] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], diluted at 1:2000.
Immunocytochemistry/ Immunofluorescence: ALDH1A3 Antibody [NBP2-15339] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ALDH1A3 stained by ALDH1A3 antibody [N2C2], diluted at 1:500. Blue: Hoechst ...read more
Immunocytochemistry/ Immunofluorescence: ALDH1A3 Antibody [NBP2-15339] - Human breast cancer cells with Aldh1a3 overexpression were stained at 1:500 in 5% goat serum in PBS-T. Human breast cancer cells were transduced ...read more
Immunohistochemistry-Paraffin: ALDH1A3 Antibody [NBP2-15339] - Mouse prostate. ALDH1A3 stained by ALDH1A3 antibody [N2C2], diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: ALDH1A3 Antibody [NBP2-15339] - Rat prostate. ALDH1A3 stained by ALDH1A3 antibody [N2C2], diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Product Details

Summary
Reactivity Hu, Mu, Rt, RMSpecies Glossary
Applications WB, IB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

ALDH1A3 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human ALDH1A3. The exact sequence is proprietary.
Localization
Cytoplasm
Predicted Species
Rhesus Macaque (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ALDH1A3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:3000
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:100 - 1:1000
  • Immunohistochemistry 1:100 - 1:1000
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100 - 1:1000
Application Notes
IHC-Fr usage reported in scientific literature (PMID: 26713822) and a verfied customer reveiw. Use in Immunoblotting reported in scientific literature (PMID: 27572106). This ALDH1A3 antibody is validated for ICC/IF from a verified customer review.
Theoretical MW
56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 3 Reviews rated 4.7
using
NBP2-15339 in the following applications:

Publications
Read Publications using
NBP2-15339 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28249000). Chicken (88%), Xenopus laevis (83%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ALDH1A3 Antibody

  • aldehyde dehydrogenase 1 family, member A3
  • Aldehyde dehydrogenase 6
  • aldehyde dehydrogenase family 1 member A3
  • ALDH1A6
  • ALDH6acetaldehyde dehydrogenase 6
  • EC 1.2.1
  • EC 1.2.1.5
  • RALDH3
  • RALDH-3
  • Retinaldehyde dehydrogenase 3

Background

Aldehyde dehydrogenase isozymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. The enzyme encoded by this gene uses retinal as a substrate, either in a free or cellular retinol-binding protein form. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ALDH1A3 Antibody (NBP2-15339)(17)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: IB, ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
IB
(1)
ICC/IF
(5)
IHC
(2)
IHC-Fr
(1)
IHC-P
(4)
WB
(2)
All Applications
Filter By Species
Human
(4)
Mouse
(10)
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using NBP2-15339 Applications Species
Stephens CH, Morrison RA, McLaughlin M et al. Oligomeric collagen as an encapsulation material for islet/beta cell replacement: Effect of islet source, dose, implant site, and administration format Am. J. Physiol. Endocrinol. Metab. Jun 16 2020 [PMID: 32543944]
Huang JL, Lee S, Hoek P et al. Genetic deletion of Urocortin 3 does not prevent functional maturation of beta cells J. Endocrinol. May 1 2020 [PMID: 32369775] (Mouse) Mouse
Shrestha N, Liu T, Ji Y et Al. Sel1L-Hrd1 ER-associated degradation maintains beta-cell identity via TGF- beta signaling J. Clin. Invest. Mar 17 2020 [PMID: 32182217] (ICC/IF, WB, Mouse) ICC/IF, WB Mouse
Liu N, Cai X, Liu T et al. Hypoxia-inducible factor-1 alpha mediates the expression of mature beta cell-disallowed genes in hypoxia-induced beta cell dedifferentiation Biochem. Biophys. Res. Commun. Dec 19 2019 [PMID: 31866014] (IHC-P, Human) IHC-P Human
GOmez-Banoy N, Guseh JS, Li G et al. Adipsin preserves beta cells in diabetic mice and associates with protection from type 2 diabetes in humans Nat. Med. Nov 1 2019 [PMID: 31700183] (IHC, IHC-P, ICC/IF, Mouse) IHC, IHC-P, ICC/IF Mouse
Rahman MT, Muppala S, Wu J et al. Induction of alpha cell-restricted Gc in dedifferentiating beta cells contributes to stress-induced beta-cell dysfunction JCI Insight May 23 2019 [PMID: 31120862] (IHC-P, Mouse) IHC-P Mouse
Tersey SA, Levasseur EM, Syed F et al. Episodic B-cell death and dedifferentiation during diet-induced obesity and dysglycemia in male mice FASEB J. May 29 2018 [PMID: 29812970] (Mouse) Mouse
Wang NN, Wang LH, Li Y et al. Targeting ALDH2 with disulfiram/copper reverses the resistance of cancer cells to microtubule inhibitors Exp. Cell Res. 2017 Nov 16 [PMID: 29155365] (Mouse) Mouse
Burke SJ, Batdorf HM, Burk DH et al. db/db Mice Exhibit Features of Human Type 2 Diabetes That Are Not Present in Weight-Matched C57BL/6J Mice Fed a Western Diet. J Diabetes Res. 2017 Oct 17 [PMID: 29038790] (ICC/IF, Mouse) ICC/IF Mouse
Ramzy A, Mojibian M, Kieffer TJ. Insulin deficient mouse b-cells do not fully mature but can be remedied through insulin replacement by islet transplantation. Endocrinology. 2017 Sep 27 [PMID: 29029025] (Mouse) Mouse
Show All 17 Publications.

Reviews for ALDH1A3 Antibody (NBP2-15339) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Mouse.

Reviews using NBP2-15339:
Filter by Applications
WB
(1)
ICC
(1)
IHC-Fr
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot ALDH1A3 NBP2-15339
Enlarge
5
reviewed by:
Anonymous
WB Human 03/08/2020
View

Summary

ApplicationWestern Blot
Sample TestedT47D human breast cancer cell line
SpeciesHuman
Lot43082
Immunocytochemistry ALDH1A3 NBP2-15339
Enlarge
4
reviewed by:
Anonymous
ICC Human 01/14/2020
View

Summary

ApplicationImmunocytochemistry
Sample TestedHuman breast cancer cells
SpeciesHuman
Lot43082

Comments

CommentsHuman breast cancer cells with Aldh1a3 overexpression were stained at 1:500 in 5% goat serum in PBS-T.
Immunohistochemistry-Frozen ALDH1A3 NBP2-15339
Enlarge
5
reviewed by:
Anonymous
IHC-Fr Mouse 11/09/2016
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedMouse Pancreas
SpeciesMouse

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ALDH1A3 Antibody (NBP2-15339) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-15339AF350
Alexa Fluor 405 NBP2-15339AF405
Alexa Fluor 488 NBP2-15339AF488
Alexa Fluor 532 NBP2-15339AF532
Alexa Fluor 594 NBP2-15339AF594
Alexa Fluor 647 NBP2-15339AF647
Alexa Fluor 700 NBP2-15339AF700
Alexa Fluor 750 NBP2-15339AF750
Biotin NBP2-15339B
DyLight 350 NBP2-15339UV
DyLight 405 NBP2-15339V
DyLight 488 NBP2-15339G
DyLight 550 NBP2-15339R
DyLight 594 NBP2-15339DL594
DyLight 650 NBP2-15339C
DyLight 680 NBP2-15339FR
DyLight 755 NBP2-15339IR
FITC NBP2-15339F
HRP NBP2-15339H
Janelia Fluor 549 NBP2-15339JF549
Janelia Fluor 646 NBP2-15339JF646

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Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

Anonymous
03/08/2020
Application: WB
Species: Human

Anonymous
01/14/2020
Application: ICC
Species: Human

Anonymous
11/09/2016
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol ALDH1A3
Uniprot