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ALDH1A3 Antibody

 (11 Publications)
 (1 Review)
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Western Blot: ALDH1A3 Antibody [NBP2-15339] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: ALDH1A3 Antibody [NBP2-15339] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], Internal diluted at 1:1000. The HRP-conjugated ...read more
Immunocytochemistry/ Immunofluorescence: ALDH1A3 Antibody [NBP2-15339] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ALDH1A3 stained by ALDH1A3 antibody [N2C2], Internal diluted at 1:500. ...read more
Immunohistochemistry-Paraffin: ALDH1A3 Antibody [NBP2-15339] - Rat prostate. ALDH1A3 stained by ALDH1A3 antibody [N2C2], Internal diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: ALDH1A3 Antibody [NBP2-15339] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], Internal diluted at 1:1000. The HRP-conjugated ...read more
Western Blot: ALDH1A3 Antibody [NBP2-15339] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], Internal diluted at 1:2000.
Western Blot: ALDH1A3 Antibody [NBP2-15339] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], Internal diluted at 1:1000. The HRP-conjugated ...read more
Immunohistochemistry-Frozen: ALDH1A3 Antibody [NBP2-15339] - 8 Week old mouse pancreas cryosections with beta cell dedifferentiation properties were stained with ALDH1A3 (1:500 dilution) and Insulin (1:1000 dilution) ...read more
Immunohistochemistry-Paraffin: ALDH1A3 Antibody [NBP2-15339] - Mouse prostate. ALDH1A3 stained by ALDH1A3 antibody [N2C2], Internal diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Product Details

Summary
Reactivity Hu, Mu, Rt, RMSpecies Glossary
Applications WB, IB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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ALDH1A3 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human ALDH1A3. The exact sequence is proprietary.
Localization
Cytoplasm
Predicted Species
Rhesus Macaque (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ALDH1A3
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:1000
Application Notes
IHC-Fr usage reported in scientific literature (PMID: 26713822). Use in Immunoblotting reported in scientific literature (PMID: 27572106).
Theoretical MW
56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-15339 in the following applications:
Publications
Read Publications using
NBP2-15339 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28249000). Chicken (88%), Xenopus laevis (83%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for ALDH1A3 Antibody

  • aldehyde dehydrogenase 1 family, member A3
  • Aldehyde dehydrogenase 6
  • aldehyde dehydrogenase family 1 member A3
  • ALDH1A6
  • ALDH6acetaldehyde dehydrogenase 6
  • EC 1.2.1
  • EC 1.2.1.5
  • RALDH3
  • RALDH-3
  • Retinaldehyde dehydrogenase 3

Background

Aldehyde dehydrogenase isozymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. The enzyme encoded by this gene uses retinal as a substrate, either in a free or cellular retinol-binding protein form. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Dehydrogenase 1-A1/AL ...
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Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP1-89152] - Analysis in human cell lines A-549 and A-431 using anti-ALDH1A1 antibody. Corresponding ALDH1A1 RNA-seq data are presented for the same cell lines. Loading control: anti-PPIB.
Immunocytochemistry/Immunofluorescence: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP1-89152] - Staining of human cell line A549 shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP1-89152] - Staining of human tonsil shows very weak positivity in non-germinal center cells.
(3 Publications)
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
ALDH1A2 Antibody
NBP1-87158
Western Blot: ALDH1A2 Antibody [NBP1-87158] - Analysis in human cell line K562.
Immunohistochemistry-Paraffin: ALDH1A2 Antibody [NBP1-87158] - Staining in human endometrium and skin tissues. Corresponding ALDH1A2 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: ALDH1A2 Antibody [NBP1-87158] - Staining of human endometrium shows moderate to strong cytoplasmic positivity in cells in endometrial stroma.
(1 Review)
(4 Publications)
Species: Hu
Applications: WB, Simple Western, IHC, IHC-P
CYP26A1 Antibody
NBP2-16061
Western Blot: CYP26A1 Antibody [NBP2-16061] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with CYP26A1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: CYP26A1 Antibody [NBP2-16061] - Mouse muscle, using CYP26A1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: CYP26A1 Antibody [NBP2-16061] - Sample (30 ug of whole cell lysate) A: 293T 7. 5% SDS PAGE gel, diluted at 1:1000.
Species: Hu, Mu
Applications: WB, IHC, IHC-P
BMP-4
[Unconjugated]
314-BP
1 ug/lane of Recombinant Human BMP-4 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing major bands at 22-25 kDa and 37-41 kDa, respectively. Multiple bands in gel are due to variable glycosylation. 
Recombinant Human BMP-4 (Catalog #314-BP/CF) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED50 for this effect is 2.5-15 ng/mL.
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Pax6 Antibody
NB300-750
Western Blot: Pax6 Antibody [NB300-750] - Analysis of sheep retinal extract
Immunocytochemistry: Pax6 Antibody [NB300-750] - ARPE-19 monolayer on glass substrate. Fixative Methanol Blocking Goat Serum. Dilution 1:100. Incubation 1h @ RT. This image was submitted via customer Review.
Western Blot: Pax6 Antibody [NB300-750] - Analysis of rat whole eye extract
(4 Reviews)
(4 Publications)
Species: Hu, Mu, Rt, Fi, Mk, Pm, Sh
Applications: WB, ICC/IF, IHC, IP, ICC
FGF-8
[Unconjugated]
423-F8
Recombinant Human/Mouse FGF-8b (Catalog # 423-F8) stimulates cell proliferation in the NR6R‑3T3 mouse fibroblast cell line. The ED50 for this effect is 6.5-40 ng/mL in the presence of 1 μg/mL heparin.
1 μg/lane of Recombinant Human/Mouse FGF-8b was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 23 kDa.
(3 Reviews)
(42 Publications)
Species: Hu, Mu
Sonic Hedgehog/Shh
[Unconjugated]
1845-SH
1 μg/lane of Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 22 kDa.
MALDI-TOF analysis of Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus. The major peak corresponds to the calculated molecular mass, 19814 Da. The minor peak at 20029  is a matrix-associated artifact of the MALDI-TOF.
Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus (Catalog # 1845-SH) induces alkaline phosphatase production by the C3H10T1/2 mouse embryonic fibroblast cell line. The ED50 for this effect is 0.1‑0.4 μg/mL.
(5 Reviews)
(31 Publications)
Species: Hu
Retinol Binding
Protein RBP Antibody ...
NBP2-00637
Western Blot: Retinol Binding Protein RBP Antibody (2H3) [NBP2-00637] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Retinol Binding Protein RBP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Retinol Binding Protein RBP.
Immunocytochemistry/Immunofluorescence: Retinol Binding Protein RBP Antibody (2H3) [NBP2-00637] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Retinol Binding Protein RBP.
Immunohistochemistry-Paraffin: Retinol Binding Protein RBP Antibody (2H3) [NBP2-00637] - Staining of paraffin-embedded Human Ovary tissue using anti-Retinol Binding Protein RBP mouse monoclonal antibody.
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Aldehyde
Dehydrogenase 3-A1/AL ...
NBP2-02483
Western Blot: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody (OTI1B6) [NBP2-02483] - analysis of extracts (10ug) from 2 different cell lines by using anti-ALDH3A1 monoclonal antibody at 1:200 dilution.
Immunocytochemistry/Immunofluorescence: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody (OTI1B6) [NBP2-02483] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ALDH3A1.
Immunohistochemistry-Paraffin: ALDH3A1 Antibody (1B6) [NBP2-02483] - Staining of paraffin-embedded prostate tissue using anti-ALDH3A1 mouse monoclonal antibody.
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
VAX2 Antibody
NBP2-15161
Western Blot: VAX2 Antibody [NBP2-15161] - Sample (30 ug of whole cell lysate) A: Jurkat 12% SDS PAGE, antibody diluted at 1:500.
Immunocytochemistry/Immunofluorescence: VAX2 Antibody [NBP2-15161] - Paraformaldehyde-fixed A431, using antibody at 1:500 dilution.
Species: Hu
Applications: WB, ICC/IF
AKR1A1 Antibody
(OTI6E3)
NBP2-02164
Western Blot: AKR1A1 Antibody (6E3) [NBP2-02164] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKR1A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKR1A1.
Immunocytochemistry/Immunofluorescence: AKR1A1 Antibody (6E3) [NBP2-02164] Staining of COS7 cells transiently transfected by pCMV6-ENTRY AKR1A1.
Immunohistochemistry-Paraffin: AKR1A1 Antibody (6E3) [NBP2-02164] - Staining of paraffin-embedded liver tissue using anti-AKR1A1 mouse monoclonal antibody.
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
ALDH9A1 Antibody
NBP1-89150
Western Blot: ALDH9A1 Antibody [NBP1-89150] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunohistochemistry-Paraffin: ALDH9A1 Antibody [NBP1-89150] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Western Blot: ALDH9A1 Antibody [NBP1-89150] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
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(C-1)
NB300-539
Immunocytochemistry/Immunofluorescence: CRABP1 Antibody (C-1) [NB300-539] - Analysis of CRABPI using Anti-CRABPI Monoclonal Antibody (C-1) shows staining in NIH-3T3 Cells. CRABPI staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing CRABPI at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunohistochemistry-Paraffin: CRABP1 Antibody (C-1) [NB300-539] - Both normal and cancer biopsies of deparaffinized Human thyroid tissues.
Flow Cytometry: CRABP1 Antibody (C-1) [NB300-539] - Analysis of CRABPI in Hela cells (green) compared to an isotype control (blue).
(5 Publications)
Species: Hu, Mu, Rt, Bv, Ca, Ch, Fe, Pm, Rb
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Cytochrome P450
26B1 Antibody (1H6)
H00056603-M01
Western Blot: Cytochrome P450 26B1 Antibody (1H6) [H00056603-M01] - Analysis of CYP26B1 expression in transfected 293T cell line by CYP26B1 monoclonal antibody (M01), clone 1H6.Lane 1: CYP26B1 transfected lysate (Predicted MW: 57.5 KDa).Lane 2: Non-transfected lysate.
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RAR alpha/NR1B1
Antibody (OTI2C3)
NBP2-45516
Western Blot: RAR alpha/NR1B1 Antibody (2C3) [NBP2-45516] - Analysis of HEK293T cells were transiently transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAR alpha.
Immunohistochemistry: RAR alpha/NR1B1 Antibody (2C3) [NBP2-45516] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120 C for 3min)
Immunohistochemistry: RAR alpha/NR1B1 Antibody (2C3) [NBP2-45516] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
(1 Publication)
Species: Hu
Applications: WB, ICC/IF, IHC
CYP1B1 Antibody
NBP2-24722
Western Blot: CYP1B1 Antibody [NBP2-24722] - Analysis of CYP1B1 in human brain lysate in the 1) absence and 2) presence of immunizing peptide using this antibody at 1 ug/ml.
Immunohistochemistry-Paraffin: CYP1B1 Antibody [NBP2-24722] - Analysis of human adrenal using this antibody at 10 ug/ml.
Immunohistochemistry-Paraffin: CYP1B1 Antibody [NBP2-24722] - Analysis of CYP1B1 in human brain tissue using an isotype control (top left) and this antibody (bottom left, right) at 5 ug/ml.
(1 Review)
(3 Publications)
Species: Hu
Applications: WB, IHC-P
Cytochrome P450
1A1/1A2 Antibody (MC1
NB100-74398
Immunocytochemistry/Immunofluorescence: Cytochrome P450 1A1/1A2 Antibody (MC1) [NB100-74398] - Analysis of Cytochrome P450 1A1/1A2 (green) showing staining in the cytoplasm and membrane of COS-7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cytochrome P450 1A1/1A2 monoclonal antibody in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI.
Immunohistochemistry-Paraffin: Cytochrome P450 1A1/1A2 Antibody (MC1) [NB100-74398] - Analysis showing positive staining in the cytoplasm and membrane of Mouse liver tissue (right) compared with a negative control in the absence of primary antibody (left).
Immunocytochemistry/Immunofluorescence: Cytochrome P450 1A1/1A2 Antibody (MC1) [NB100-74398] - Analysis of Cytochrome P450 1A1/1A2 (green) showing staining in the cytoplasm and membrane of HT29 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cytochrome P450 1A1/1A2 monoclonal antibody in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescentred phalloidin and nuclei (blue) were stained with Hoechst or DAPI.
(1 Review)
(1 Publication)
Species: Hu, Mu, Rt, Pm
Applications: WB, ICC/IF, IHC, IHC-P
ALDH1A3 Antibody
NBP2-15339
ALDH1A3 Antibody
Species: Hu, Mu, Rt, RM
Applications: WB, IB, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for ALDH1A3 Antibody (NBP2-15339)(11)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: IB, ICC/IF, IHC, IHC-Fr, IHC-P, WB.


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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NBP2-15339 Applications Species
Rahman MT, Muppala S, Wu J et al. Induction of alpha cell-restricted Gc in dedifferentiating beta cells contributes to stress-induced beta-cell dysfunction JCI Insight May 23 2019 [PMID: 31120862] (IHC-P, Mouse) IHC-P Mouse
Tersey SA, Levasseur EM, Syed F et al. Episodic B-cell death and dedifferentiation during diet-induced obesity and dysglycemia in male mice FASEB J. May 29 2018 [PMID: 29812970] (Mouse) Mouse
Wang NN, Wang LH, Li Y et al. Targeting ALDH2 with disulfiram/copper reverses the resistance of cancer cells to microtubule inhibitors Exp. Cell Res. 2017 Nov 16 [PMID: 29155365] (Mouse) Mouse
Burke SJ, Batdorf HM, Burk DH et al. db/db Mice Exhibit Features of Human Type 2 Diabetes That Are Not Present in Weight-Matched C57BL/6J Mice Fed a Western Diet. J Diabetes Res. 2017 Oct 17 [PMID: 29038790] (ICC/IF, Mouse) ICC/IF Mouse
Ramzy A, Mojibian M, Kieffer TJ. Insulin deficient mouse b-cells do not fully mature but can be remedied through insulin replacement by islet transplantation. Endocrinology. 2017 Sep 27 [PMID: 29029025] (Mouse) Mouse
Li M, Xiao A, Floyd D et al. CDK4/6 inhibition is more active against the glioblastoma proneural subtype Oncotarget 2017 Aug 15 [PMID: 28903422] (WB, Human) WB Human
Ishida E, kim-Muller JY, Accili D. Pair Feeding, but Not Insulin, Phloridzin, or Rosiglitazone Treatment, Curtails Markers of B-Cell Dedifferentiation in db/db Mice. Diabetes. Aug 1 2017 [PMID: 28506962] (ICC/IF, Mouse) ICC/IF Mouse
Feng AL, Xiang YY, Gui L et al. Paracrine GABA and insulin regulate pancreatic alpha cell proliferation in a mouse model of type 1 diabetes. Diabetologia. Mar 9 2017 [PMID: 28280900] (Mouse) Mouse
Kim-Muller Jy, Fan J, Kim Yj et al. Aldehyde dehydrogenase 1a3 defines a subset of failing pancreatic beta cells in diabetic mice. Nat Commun Aug 30 2016 [PMID: 27572106] (IHC, IB) IHC, IB
Cinti F, Bouchi R, Kim-Muller JY et al. Evidence of B-cell Dedifferentiation in Human Type 2 Diabetes. J. Clin. Endocrinol. Metab. 2015 Dec 29 [PMID: 26713822] (IHC-Fr, Human) IHC-Fr Human
Show All 11 Publications.

Review for ALDH1A3 Antibody (NBP2-15339) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP2-15339:
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Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen ALDH1A3 NBP2-15339
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reviewed by:
Anonymous
 IHC-Fr Mouse 11/09/2016
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedMouse Pancreas
SpeciesMouse

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Immunohistochemistry-Frozen ALDH1A3 NBP2-15339
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 Anonymous
11/09/2016
Application: IHC-Fr
Species: Mouse
read this review

Bioinformatics

Gene Symbol ALDH1A3
Uniprot