ALDH1A3 Antibody - BSA Free

Images

 
Western Blot: ALDH1A3 Antibody - BSA Free [NBP2-15339] - Total protein from human Lymph node, mouse Stomach and A431 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat ...read more
Immunocytochemistry/ Immunofluorescence: ALDH1A3 Antibody - BSA Free [NBP2-15339] - A431 cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were ...read more
Immunohistochemistry: ALDH1A3 Antibody - BSA Free [NBP2-15339] - Markers of dedifferentiation are present in islets of db/db, but not weight-matched WD-fed mice. Staining for Aldh1a3 protein (red) and insulin (green) in ...read more
Western Blot: ALDH1A3 Antibody - BSA Free [NBP2-15339] - IL25 Deficiency Induced loss of CRC stemness. The expression levels of CSC markers, namely, DCLK1, ALDH1 (NBP2-15339), and CD44, were examined in tumor tissues by ...read more
Western Blot: ALDH1A3 Antibody - BSA Free [NBP2-15339] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], diluted at 1:1000. The HRP-conjugated ...read more
Western Blot: ALDH1A3 Antibody - BSA Free [NBP2-15339] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], diluted at 1:2000.
Immunocytochemistry/ Immunofluorescence: ALDH1A3 Antibody - BSA Free [NBP2-15339] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ALDH1A3 stained by ALDH1A3 antibody [N2C2], diluted at 1:500. ...read more
Immunocytochemistry/ Immunofluorescence: ALDH1A3 Antibody - BSA Free [NBP2-15339] - Human breast cancer cells with Aldh1a3 overexpression were stained at 1:500 in 5% goat serum in PBS-T. Human breast cancer cells were ...read more
Immunohistochemistry-Frozen: ALDH1A3 Antibody - BSA Free [NBP2-15339] - 8-week old mouse pancreas cryosections with beta cell dedifferentiation properties were stained with ALDH1A3 (1:500 dilution) and Insulin (1:1000 ...read more
Immunohistochemistry-Paraffin: ALDH1A3 Antibody - BSA Free [NBP2-15339] - Mouse prostate. ALDH1A3 stained by ALDH1A3 antibody [N2C2], diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: ALDH1A3 Antibody - BSA Free [NBP2-15339] - Rat prostate. ALDH1A3 stained by ALDH1A3 antibody [N2C2], diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Palbociclib induces a potential transition of PN GSCs to MES GSCs. (B) qPCR and immunoblot analysis of ALDH1A3 in three independent PN GSC lines that were treated with 10 nM of palbociclib for 5 days. n = 3. Differences ...read more
Markers of dedifferentiation are present in islets of db/db, but not weight-matched WD-fed mice. (a) Immunofluorescent analysis of islets from weight-matched WD-fed mice (top row) and db/db mice (bottom row) showing ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, RMSpecies Glossary
Applications WB, ICC/IF, IHC, WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

ALDH1A3 Antibody - BSA Free Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human ALDH1A3. The exact sequence is proprietary.
Localization
Cytoplasm
Predicted Species
Rhesus Macaque (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ALDH1A3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoblotting reported in scientific literature (PMID 27572106)
  • Immunocytochemistry/ Immunofluorescence 1:100 - 1:1000. Use reported by customer review
  • Immunohistochemistry 1:100 - 1:1000
  • Immunohistochemistry-Frozen reported by customer review and in scientific literature (PMID 26713822)
  • Immunohistochemistry-Paraffin 1:100 - 1:1000
  • Western Blot 1:500 - 1:3000
Theoretical MW
56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 3 Reviews rated 4.7
using
NBP2-15339 in the following applications:

Publications
Read Publications using
NBP2-15339 in the following applications:

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:34572374). Mouse reactivity reported in scientific literature (PMID: 28249000). Chicken (88%)., Xenopus laevis (83%). .

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ALDH1A3 Antibody - BSA Free

  • aldehyde dehydrogenase 1 family, member A3
  • Aldehyde Dehydrogenase 1-A3
  • Aldehyde dehydrogenase 6
  • aldehyde dehydrogenase family 1 member A3
  • ALDH1A3
  • ALDH1A6
  • ALDH6
  • ALDH6acetaldehyde dehydrogenase 6
  • EC 1.2.1
  • EC 1.2.1.5
  • MCOP8
  • RALDH3
  • RALDH-3
  • Retinaldehyde dehydrogenase 3

Background

Aldehyde dehydrogenase isozymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. The enzyme encoded by this gene uses retinal as a substrate, either in a free or cellular retinol-binding protein form. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF5869
Species: Hu, Mu
Applications: ICC, IHC, IP, Simple Western, WB
NBP1-87158
Species: Hu
Applications: IHC, IHC-P, Simple Western, WB
NBP3-16627
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
314-BP
Species: Hu
Applications: BA, BA
NB300-750
Species: Fi, Hu, Pm, Mu, Pm, Rt, Sh
Applications: ICC/IF, IHC, IP, WB
423-F8
Species: Hu, Mu
Applications: BA
464-SH/CF
Species: Mu
Applications: BA
NBP2-00637
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP2-02483
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP2-15161
Species: Hu
Applications: ICC/IF, WB
NBP2-02164
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP1-89150
Species: Hu, Rt
Applications: IHC, IHC-P, WB
NBP3-16601
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NB100-53810
Species: Hu, Mu
Applications: Flow, IHC, IHC-P, PEP-ELISA
NB200-322
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, WB
NBP2-24722
Species: Hu
Applications: IHC, IHC-P, In vitro, WB
NB100-74398
Species: Hu, Mu, Pm, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-15339
Species: Hu, Mu, Rt, RM
Applications: WB, ICC/IF, IHC, WB

Publications for ALDH1A3 Antibody (NBP2-15339)(34)

We have publications tested in 3 confirmed species: Human, Mouse, Rhesus Macaque.

We have publications tested in 8 applications: IB, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, Simple Western, WB.


Filter By Application
IB
(1)
ICC/IF
(7)
IF/IHC
(3)
IHC
(2)
IHC-Fr
(2)
IHC-P
(7)
Simple Western
(1)
WB
(7)
All Applications
Filter By Species
Human
(7)
Mouse
(21)
Rhesus Macaque
(1)
All Species
Showing Publications 1 - 10 of 34. Show All 34 Publications.
Publications using NBP2-15339 Applications Species
Bauer BM, Irimia JM, Bloom-Saldana E et al. Pancreatic loss of Mig6 alters murine endocrine cell fate and protects functional beta cell mass in an STZ-induced model of diabetes bioRxiv : the preprint server for biology 2023-04-08 [PMID: 37066257] (Simple Western, Mouse) Simple Western Mouse
Son J, Du W, Esposito M et al. Genetic and pharmacologic inhibition of ALDH1A3 as a treatment of ?-cell failure Nature communications 2023-02-02 [PMID: 36732513] (IHC) IHC
Steingruber L, Krabichler F, Franzmeier S et al. Aldehyde Dehydrogenase 1 paralogues ALDH1A1 and ALDH1A3 in satellite cells are essential for myogenic differentiation Research Square 2022-10-26 (ICC/IF, WB, Mouse) ICC/IF, WB Mouse
Osipovich AB, Zhou FY, Chong JJ et al. Deletion of Ascl1 in pancreatic ?-cells improves insulin secretion, promotes parasympathetic innervation, and attenuates dedifferentiation during metabolic stress Molecular Metabolism 2023-09-01 [PMID: 37769990] (IHC, Mouse) IHC Mouse
Chen Z, Will R, Kim SN et al. Novel Function of Cancer Stem Cell Marker ALDH1A3 in Glioblastoma: Pro-Angiogenesis through Paracrine PAI-1 and IL-8 Cancers (Basel) 2023-09-04 [PMID: 37686698]
Wang K, Cui X, Li F et al. Glucagon receptor blockage inhibits beta -cell dedifferentiation through FoxO1 American journal of physiology. Endocrinology and metabolism 2022-11-16 [PMID: 36383639] (IHC-P, Mouse) IHC-P Mouse
Alghamdi TA, Krentz NAJ, Smith N et al. Zmiz1 is required for mature B-cell function and mass expansion upon high fat feeding Molecular metabolism 2022-10-25 [PMID: 36307047] (WB, Mouse)

Details:
Dilution used in WB 1:200
WB Mouse
Tsuchiya T, Saisho Y, Inaishi J et al. Increased alpha cell to beta cell ratio in patients with pancreatic cancer Endocrine journal 2022-08-06 [PMID: 35934795] (IHC-P, Human)

Details:
Dilution used for IHC 1:1000
IHC-P Human
Liu J, Qian B, Zhou L et al. IL25 Enhanced Colitis-Associated Tumorigenesis in Mice by Upregulating Transcription Factor GLI1 Frontiers in Immunology 2022-03-14 [PMID: 35359953] (WB, Mouse) WB Mouse
Show All 34 Publications.

Reviews for ALDH1A3 Antibody (NBP2-15339) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Mouse.

Reviews using NBP2-15339:
Filter by Applications
WB
(1)
ICC
(1)
IHC-Fr
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot ALDH1A3 NBP2-15339
Enlarge
5
reviewed by:
Verified Customer
WB Human 03/08/2020
View

Summary

ApplicationWestern Blot
Sample TestedT47D human breast cancer cell line
SpeciesHuman
Lot43082
Immunocytochemistry ALDH1A3 NBP2-15339
Enlarge
4
reviewed by:
Verified Customer
ICC Human 01/14/2020
View

Summary

ApplicationImmunocytochemistry
Sample TestedHuman breast cancer cells
SpeciesHuman
Lot43082

Comments

CommentsHuman breast cancer cells with Aldh1a3 overexpression were stained at 1:500 in 5% goat serum in PBS-T.
Immunohistochemistry-Frozen ALDH1A3 NBP2-15339
Enlarge
5
reviewed by:
Verified Customer
IHC-Fr Mouse 11/09/2016
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedMouse Pancreas
SpeciesMouse

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ALDH1A3 Antibody (NBP2-15339) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional ALDH1A3 Products

Diseases for ALDH1A3 Antibody (NBP2-15339)

Discover more about diseases related to ALDH1A3 Antibody (NBP2-15339).
 

Pathways for ALDH1A3 Antibody (NBP2-15339)

View related products by pathway.

PTMs for ALDH1A3 Antibody (NBP2-15339)

Learn more about PTMs related to ALDH1A3 Antibody (NBP2-15339).
 

Research Areas for ALDH1A3 Antibody (NBP2-15339)

Find related products by research area.

Blogs on ALDH1A3

There are no specific blogs for ALDH1A3, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

Verified Customer
03/08/2020
Application: WB
Species: Human

Verified Customer
01/14/2020
Application: ICC
Species: Human

Verified Customer
11/09/2016
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol ALDH1A3
Uniprot