New pricing — Effective July 1, 2022 -

Amidst rising costs across all areas of our business, and aggressive attempts to implement cost savings without sacrificing quality, we announce the need to implement a cost increase starting July 1, 2022. If you have questions, please contact your sales representative.

ALDH1A3 Antibody - BSA Free

Images

 
Immunohistochemistry-Frozen: ALDH1A3 Antibody [NBP2-15339] - 8-week old mouse pancreas cryosections with beta cell dedifferentiation properties were stained with ALDH1A3 (1:500 dilution) and Insulin (1:1000 dilution) ...read more
Western Blot: ALDH1A3 Antibody [NBP2-15339] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], diluted at 1:1000. The HRP-conjugated anti-rabbit IgG ...read more
Immunohistochemistry: ALDH1A3 Antibody [NBP2-15339] - Markers of dedifferentiation are present in islets of db/db, but not weight-matched WD-fed mice. Staining for Aldh1a3 protein (red) and insulin (green) in islets ...read more
Western Blot: ALDH1A3 Antibody [NBP2-15339] - Total protein from human Lymph node, mouse Stomach and A431 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in ...read more
Immunocytochemistry/ Immunofluorescence: ALDH1A3 Antibody [NBP2-15339] - A431 cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated ...read more
Western Blot: ALDH1A3 Antibody [NBP2-15339] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], diluted at 1:2000.
Immunocytochemistry/ Immunofluorescence: ALDH1A3 Antibody [NBP2-15339] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ALDH1A3 stained by ALDH1A3 antibody [N2C2], diluted at 1:500. Blue: Hoechst ...read more
Immunocytochemistry/ Immunofluorescence: ALDH1A3 Antibody [NBP2-15339] - Human breast cancer cells with Aldh1a3 overexpression were stained at 1:500 in 5% goat serum in PBS-T. Human breast cancer cells were transduced ...read more
Immunohistochemistry-Paraffin: ALDH1A3 Antibody [NBP2-15339] - Mouse prostate. ALDH1A3 stained by ALDH1A3 antibody [N2C2], diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: ALDH1A3 Antibody [NBP2-15339] - Rat prostate. ALDH1A3 stained by ALDH1A3 antibody [N2C2], diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Product Details

Summary
Reactivity Hu, Mu, Rt, RMSpecies Glossary
Applications WB, IB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

ALDH1A3 Antibody - BSA Free Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human ALDH1A3. The exact sequence is proprietary.
Localization
Cytoplasm
Predicted Species
Rhesus Macaque (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ALDH1A3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:100 - 1:1000
  • Immunohistochemistry 1:100 - 1:1000
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100 - 1:1000
  • Western Blot 1:500 - 1:3000
Application Notes
IHC-Fr usage reported in scientific literature (PMID: 26713822) and a verfied customer reveiw. Use in Immunoblotting reported in scientific literature (PMID: 27572106). This ALDH1A3 antibody is validated for ICC/IF from a verified customer review.
Theoretical MW
56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 3 Reviews rated 4.7
using
NBP2-15339 in the following applications:

Publications
Read Publications using
NBP2-15339 in the following applications:

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:34572374). Mouse reactivity reported in scientific literature (PMID: 28249000). Chicken (88%)., Xenopus laevis (83%). .

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ALDH1A3 Antibody - BSA Free

  • aldehyde dehydrogenase 1 family, member A3
  • Aldehyde dehydrogenase 6
  • aldehyde dehydrogenase family 1 member A3
  • ALDH1A6
  • ALDH6acetaldehyde dehydrogenase 6
  • EC 1.2.1
  • EC 1.2.1.5
  • RALDH3
  • RALDH-3
  • Retinaldehyde dehydrogenase 3

Background

Aldehyde dehydrogenase isozymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. The enzyme encoded by this gene uses retinal as a substrate, either in a free or cellular retinol-binding protein form. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF5869
Species: Hu, Mu
Applications: ICC, IHC, IP, Simple Western, WB
NBP1-87158
Species: Hu
Applications: IHC, IHC-P, Simple Western, WB
NBP3-16627
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
314-BP/CF
Species: Hu
Applications: BA, BA
NB300-750
Species: Fi, Hu, Pm, Mu, Pm, Rt, Sh
Applications: ICC/IF, IHC, IP, WB
423-F8
Species: Hu, Mu
Applications: BA
1845-SH/CF
Species: Hu
Applications: BA
NBP2-00637
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP2-02483
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP2-15161
Species: Hu
Applications: ICC/IF, WB
NBP2-02164
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP1-89150
Species: Hu, Rt
Applications: IHC, IHC-P, WB
NB300-539
Species: Bv, Ca, Ch, Fe, Hu, Mu, Pm, Rb, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, WB
NB100-53810
Species: Hu, Mu
Applications: Flow, IHC, IHC-P, PEP-ELISA
NB200-322
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, WB
NBP2-24722
Species: Hu
Applications: IHC, IHC-P, In vitro, WB
NB100-74398
Species: Hu, Mu, Pm, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-15339
Species: Hu, Mu, Rt, RM
Applications: WB, IB, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for ALDH1A3 Antibody (NBP2-15339)(26)

We have publications tested in 3 confirmed species: Human, Mouse, Rhesus Macaque.

We have publications tested in 6 applications: IB, ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
IB
(1)
ICC/IF
(6)
IHC
(3)
IHC-Fr
(2)
IHC-P
(5)
WB
(5)
All Applications
Filter By Species
Human
(6)
Mouse
(16)
Rhesus Macaque
(1)
All Species
Showing Publications 1 - 10 of 26. Show All 26 Publications.
Publications using NBP2-15339 Applications Species
Liu J, Qian B, Zhou L et al. IL25 Enhanced Colitis-Associated Tumorigenesis in Mice by Upregulating Transcription Factor GLI1 Frontiers in Immunology Mar 14 2022 [PMID: 35359953] (WB, Mouse) WB Mouse
LOpez-PErez A, Norlin S, Steneberg P et al. Pan-AMPK activator O304 prevents gene expression changes and remobilisation of histone marks in islets of diet-induced obese mice Scientific reports Dec 23 2021 [PMID: 34949756] (ICC/IF, Mouse) ICC/IF Mouse
Brown M, Holmes H, Rakshit K et al. Electrogenic sodium bicarbonate cotransporter NBCe1 regulates pancreatic Beta cell function in type 2 diabetes Journal of Clinical Investigation Sep 1 2021 [PMID: 34623331] (IHC-P, Mouse) IHC-P Mouse
Shibata M, Pattabiraman K, Lorente-Galdos B Et al. Regulation of prefrontal patterning and connectivity by retinoic acid Nature Oct 1 2021 [PMID: 34599305] (WB, Human, Rhesus Macaque, Mouse) WB Human, Rhesus Macaque, Mouse
Collier JJ, Batdorf HM, Merrifield KL et al. Pioglitazone Reverses Markers of Islet Beta-Cell De-Differentiation in db/db Mice While Modulating Expression of Genes Controlling Inflammation and Browning in White Adipose Tissue from Insulin-Resistant Mice and Humans Biomedicines Sep 10 2021 [PMID: 34572374] (IHC, Mouse) IHC Mouse
Shrestha N, Liu T, Ji Y et Al. Sel1L-Hrd1 ER-associated degradation maintains beta-cell identity via TGF- beta signaling J. Clin. Invest. Mar 17 2020 [PMID: 32182217] (ICC/IF, WB, Mouse) ICC/IF, WB Mouse
Koike S, Hsu MF, Bettaieb A et al. Genetic deficiency or pharmacological inhibition of soluble epoxide hydrolase ameliorates high fat diet-induced pancreatic beta-cell dysfunction and loss Free radical biology & medicine May 23 2021 [PMID: 34038767]
Li B, Cheng Y, Yin Y et al. Reversion of early- and late-stage beta-cell dedifferentiation by human umbilical cord-derived mesenchymal stem cells in type 2 diabetic mice Cytotherapy Mar 15 2021 [PMID: 33736932] (IHC-Fr, Mouse) IHC-Fr Mouse
Lehman BJ, Lopez-Diaz FJ, Santisakultarm TP, et al. Dynamic regulation of CTCF stability and sub-nuclear localization in response to stress PLoS genetics Jan 1 2021 [PMID: 33411704] (WB, Human) WB Human
Show All 26 Publications.

Reviews for ALDH1A3 Antibody (NBP2-15339) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Mouse.

Reviews using NBP2-15339:
Filter by Applications
WB
(1)
ICC
(1)
IHC-Fr
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot ALDH1A3 NBP2-15339
Enlarge
5
reviewed by:
Verified Customer
WB Human 03/08/2020
View

Summary

ApplicationWestern Blot
Sample TestedT47D human breast cancer cell line
SpeciesHuman
Lot43082
Immunocytochemistry ALDH1A3 NBP2-15339
Enlarge
4
reviewed by:
Verified Customer
ICC Human 01/14/2020
View

Summary

ApplicationImmunocytochemistry
Sample TestedHuman breast cancer cells
SpeciesHuman
Lot43082

Comments

CommentsHuman breast cancer cells with Aldh1a3 overexpression were stained at 1:500 in 5% goat serum in PBS-T.
Immunohistochemistry-Frozen ALDH1A3 NBP2-15339
Enlarge
5
reviewed by:
Verified Customer
IHC-Fr Mouse 11/09/2016
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedMouse Pancreas
SpeciesMouse

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ALDH1A3 Antibody (NBP2-15339) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional ALDH1A3 Products

Bioinformatics Tool for ALDH1A3 Antibody (NBP2-15339)

Discover related pathways, diseases and genes to ALDH1A3 Antibody (NBP2-15339). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ALDH1A3 Antibody (NBP2-15339)

Discover more about diseases related to ALDH1A3 Antibody (NBP2-15339).
 

Pathways for ALDH1A3 Antibody (NBP2-15339)

View related products by pathway.

PTMs for ALDH1A3 Antibody (NBP2-15339)

Learn more about PTMs related to ALDH1A3 Antibody (NBP2-15339).
 

Research Areas for ALDH1A3 Antibody (NBP2-15339)

Find related products by research area.

Blogs on ALDH1A3

There are no specific blogs for ALDH1A3, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

Verified Customer
03/08/2020
Application: WB
Species: Human

Verified Customer
01/14/2020
Application: ICC
Species: Human

Verified Customer
11/09/2016
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol ALDH1A3
Uniprot