Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [Alexa Fluor® 350]

• Applications: WB, IHC, IP, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 350

Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant human Aldehyde Dehydrogenase 1-A1/ALDH1A1
  Ser2-Ser501
  Accession # P00352
• Specificity: Detects human Aldehyde Dehydrogenase 1‑A1/ALDH1A1 in direct ELISAs and detects human and mouse Aldehyde Dehydrogenase 1‑A1/ALDH1A1 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Aldehyde dehydrogenases (ALDHs) are NAD(P)⁺-dependent enzymes that detoxify aldehydes by oxidizing them to carboxylic acids. Nineteen ALDHs are present in humans, expressed in a variety of organelles and having different substrate preferences (1). ALDH1A1 is a cytosolic enzyme that preferentially oxidizes retinaldehyde to retinoic acid (2). ALDH1A1 is expressed in the epithelium of many organs, including brain, liver, testis, eye lens and cornea (3). ALDH1A1 is highly expressed in brain dopaminergic neurons, where it produces the retinoic acid required for their differentiation and development (4). The retinoic acid produced by ALDH1A1 is also important for the differentiation of hematopoietic stem cells (5). ALDH1A1 is a major enzyme in the oxidation of acetaldehyde, a toxic metabolite of ethanol (6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.