ALCAM/CD166 Antibody (105902)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready, ELISA(Cap)
Clone
105902
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

ALCAM/CD166 Antibody (105902) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human ALCAM/CD166
Trp28-Ala526
Accession # Q13740
Specificity
Detects human ALCAM/CD166 in Western blots. Shows approximately 50% cross‑reactivity with recombinant mouse OCAM and no cross‑reactivity with recombinant human (rh) BCAM, rhEpCAM, rhMCAM, or rhNCAM-L1.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ALCAM
Purity
Protein A or G purified from ascites
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Detection: Human ALCAM/CD166 Biotinylated Antibody (Catalog number BAF656)
Standard: Recombinant Human ALCAM/CD166 Fc Chimera (Catalog number 656-AL)
Publications
Read Publications using
MAB6561 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Purity
Protein A or G purified from ascites
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ALCAM/CD166 Antibody (105902)

  • activated leucocyte cell adhesion molecule
  • activated leukocyte cell adhesion moleculeFLJ38514
  • ALCAM
  • CD166 antigen
  • CD166
  • MEMDMGC71733

Background

ALCAM, activated leukocyte cell adhesion molecule, is a type I membrane glycoprotein and a member of the immunoglobulin supergene family. It is also known as CD166, MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. ALCAM is expressed on thymic epithelial cells, activated B and T cells, and monocytes. ALCAM can bind itself homotypically and is also capable of binding CD6, NgCAM, and other, as of yet, unidentified brain proteins. The ALCAM/CD6 interaction may be involved in T cell development and T cell regulation. Additionally, ALCAM/CD6 and ALCAM/NgCAM interactions may play roles in the nervous system. ALCAM has also been observed to be upregulated on highly metastasizing melanoma cell lines and may play a role in tumor migration. ALCAM is a 583 amino acid (aa) protein consisting of a 27 aa signal peptide, a 500 aa extracellular domain, a 24 aa transmembrane domain and a 32 aa cytoplasmic domain. The extracellular domain of ALCAM contains 5 Ig-like domains.

  1. Bowen, M.A. et al. (1995) J. Exp. Med. 181:2213.
  2. Aruffo, A. et al. (1997) Immunol. Today 18:498.
  3. Degen, W.G. et al. (1998) Am. J. Pathol. 152:805.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ALCAM/CD166 Antibody (MAB6561)(8)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 3 applications: ELISA Development, Flow Cytometry, Western Blot.


Filter By Application
ELISA Development
(2)
Flow Cytometry
(5)
Western Blot
(1)
All Applications
Filter By Species
Human
(7)
Rat
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using MAB6561 Applications Species
U Valentiner, J Knips, R Pries, T Clauditz, A Münscher, G Sauter, B Wollenberg, U Schumacher Selectin Binding Sites Are Involved in Cell Adhesive Properties of Head and Neck Squamous Cell Carcinoma Cancers (Basel), 2019;11(11):. 2019 [PMID: 31661833] (Flow Cytometry, Human) Flow Cytometry Human
SM Koura, M Salama, M El-Hussiny, MEA Khalil, A Lotfy, SA Hassan, SA Gad Elhak, MA Sobh Fluoxetine induces direct inhibitory effects on mesenchymal stem cell?derived osteoprogenitor cells independent of serotonin concentration Mol Med Rep, 2019;0(0):. 2019 [PMID: 30720108] (Flow Cytometry, Rat) Flow Cytometry Rat
KE Hebron, EY Li, SA Arnold Egl, AK von Lersne, C Taylor, J Houkes, DK Flaherty, A Eskaros, TP Stricker, A Zijlstra Alternative splicing of ALCAM enables tunable regulation of cell-cell adhesion through differential proteolysis Sci Rep, 2018;8(1):3208. 2018 [PMID: 29453336] (Flow Cytometry, Human) Flow Cytometry Human
Williams D, Anastos K, Morgello S, Berman J JAM-A and ALCAM are therapeutic targets to inhibit diapedesis across the BBB of CD14+CD16+ monocytes in HIV-infected individuals. J Leukoc Biol, 2015;97(2):401-12. 2015 [PMID: 25420915] (Flow Cytometry, Human) Flow Cytometry Human
Hansen A, Freeman T, Arnold S, Starchenko A, Jones-Paris C, Gilger M, Washington M, Fan K, Shyr Y, Beauchamp R, Zijlstra A Elevated ALCAM shedding in colorectal cancer correlates with poor patient outcome. Cancer Res, 2013;73(10):2955-64. 2013 [PMID: 23539446] (Western Blot, Human) Western Blot Human
Ihnen M, Kress K, Kersten JF, Kilic E, Choschzick M, Zander H, Muller V, Mahner S, Janicke F, Woelber L, Milde-Langosch K Relevance of activated leukocyte cell adhesion molecule (ALCAM) in tumor tissue and sera of cervical cancer patients. BMC Cancer, 2012;12(0):140. 2012 [PMID: 22475274] (ELISA Development, Human) ELISA Development Human
Kulasingam V, Zheng Y, Soosaipillai A, Leon AE, Gion M, Diamandis EP Activated leukocyte cell adhesion molecule: a novel biomarker for breast cancer. Int. J. Cancer, 2009;125(1):9-14. 2009 [PMID: 19322904] (ELISA Development, Human) ELISA Development Human
Dalerba P, Dylla S, Park I, Liu R, Wang X, Cho R, Hoey T, Gurney A, Huang E, Simeone D, Shelton A, Parmiani G, Castelli C, Clarke M Phenotypic characterization of human colorectal cancer stem cells. Proc Natl Acad Sci U S A, 0;104(24):10158-63. 0 [PMID: 17548814] (Flow Cytometry, Human) Flow Cytometry Human

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ALCAM
Uniprot