ALCAM/CD166 Antibody (105901) [Unconjugated]

Western blot shows lysates of HuT 78 human cutaneous T cell lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ALCAM/CD166 Monoclonal Antibody (Catalog # MAB656) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ALCAM/CD166 at approximately 120 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1. For best results, non-reducing conditions only are recommended.

• Reactivity: Hu
• Applications: WB, B/N
• Clone: 105901
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

ALCAM/CD166 Antibody (105901) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ALCAM/CD166
  Trp28-Ala526
  Accession # Q13740
• Specificity: Detects human ALCAM/CD166 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) BCAM, recombinant mouse OCAM, rhMCAM, or rhEpCAM is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALCAM
• Purity Statement: Protein A or G purified from ascites
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Adhesion Blockade
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ALCAM/CD166 Antibody (105901) [Unconjugated]

• activated leucocyte cell adhesion molecule
• activated leukocyte cell adhesion moleculeFLJ38514
• ALCAM
• CD166 antigen
• CD166
• MEMDMGC71733

Background

ALCAM, activated leukocyte cell adhesion molecule, is a type I membrane glycoprotein and a member of the immunoglobulin supergene family. It is also known as CD166, MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. ALCAM is expressed on thymic epithelial cells, activated B and T cells, and monocytes. ALCAM can bind itself homotypically and is also capable of binding CD6, NgCAM, and other, as of yet, unidentified brain proteins. The ALCAM/CD6 interaction may be involved in T cell development and T cell regulation. Additionally, ALCAM/CD6 and ALCAM/NgCAM interactions may play roles in the nervous system. ALCAM has also been observed to be upregulated on highly metastasizing melanoma cell lines and may play a role in tumor migration. ALCAM is a 583 amino acid (aa) protein consisting of a 27 aa signal peptide, a 500 aa extracellular domain, a 24 aa transmembrane domain and a 32 aa cytoplasmic domain. The extracellular domain of ALCAM contains 5 Ig-like domains.

1. Bowen, M.A. et al. (1995) J. Exp. Med. 181:2213.
2. Aruffo, A. et al. (1997) Immunol. Today 18:498.
3. Degen, W.G. et al. (1998) Am. J. Pathol. 152:805.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ALCAM/CD166 Antibody (MAB656)(5)

Publications using MAB656

• G Enyindah-A, Y Li, JH Ruth, DS Spassov, KE Hebron, A Zijlstra, MM Moasser, B Wang, NG Singer, H Cui, RA Ohara, SM Rasmussen, DA Fox, F Lin CD318 is a ligand for CD6 Proc. Natl. Acad. Sci. U.S.A., 2017-07-31;0(0):. 2017-07-31 [PMID: 28760953] (Functional Assay, Human)
• Transcriptional and functional characterization of CD137L-dendritic cells identifies a novel dendritic cell phenotype Sci Rep, 2016-07-19;6(0):29712. 2016-07-19 [PMID: 27431276] (Functional Assay, Human)
• Nina Stojcheva, Gennadi Schechtmann, Steffen Sass, Patrick Roth, Ana-Maria Florea, Anja Stefanski et al. MicroRNA-138 promotes acquired alkylator resistance in glioblastoma by targeting the Bcl-2-interacting mediator BIM Oncotarget 3/15/2016 [PMID: 26887050]
• Yao H, Kim K, Duan M, Hayashi T, Guo M, Morgello S, Prat A, Wang J, Su TP, Buch S Cocaine hijacks sigma1 receptor to initiate induction of activated leukocyte cell adhesion molecule: implication for increased monocyte adhesion and migration in the CNS. J. Neurosci., 2011-04-20;31(16):5942-55. 2011-04-20 [PMID: 21508219] (Neutralization, Mouse)
• Vermeulen L, De Sousa E Melo F, van der Heijden M, Cameron K, de Jong JH, Borovski T, Tuynman JB, Todaro M, Merz C, Rodermond H, Sprick MR, Kemper K, Richel DJ, Stassi G, Medema JP Wnt activity defines colon cancer stem cells and is regulated by the microenvironment. Nat. Cell Biol., 2010-04-25;12(5):468-76. 2010-04-25 [PMID: 20418870] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: ALCAM
• Uniprot:
  • Q13740()