Western Blot: Akt1 [p Ser473] Antibody [NB600-590] - Lane 1: AKT1 Recombinant Protein. Lane 2: AKT1 Mutant Human Recombinant Protein. Lane 3: AKT1 (phosphatase treated) Human Recombinant Protein. Load: 50 ng per lane. ...read more
Immunocytochemistry/ Immunofluorescence: Akt1 [p Ser473] Antibody [NB600-590] - Tissue: cardiomyocytes infected with adenovirus expressing with wild-type AKT. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary ...read more
Immunohistochemistry: Akt1 [p Ser473] Antibody [NB600-590] - Tissue: human breast carcinoma. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Akt pS473 antibody at 100 ...read more
Western Blot: Akt1 [p Ser473] Antibody [NB600-590] - Lane 1: nuclear extract from cells infected with adenovirus expressing nuclear-targeted AKT kinase. Load: 35 ug per lane. Primary antibody: AKT pS473 antibody at ...read more
Immunohistochemistry-Paraffin: Akt1 [p Ser473] Antibody [NB600-590] - Rabbit anti-AKT pS473 was used at a 1:100 dilution to detect AKT in human breast tumor tissue. The staining is much stronger than the weak basal ...read more
Immunohistochemistry-Paraffin: Akt1 [p Ser473] Antibody [NB600-590] - Rabbit used at a 1:100 dilution to detect AKT in normal human breast tissue. AKT is weakly phosphorylated in normal tissue in the breast. The ...read more
Predicted cross-reactivity based on sequence identity: Chicken.
Packaging, Storage & Formulations
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% Sodium Azide
Immunogen affinity purified
Alternate Names for AKT1 [p Ser473] Antibody
Protein kinase B
rac protein kinase alpha
RAC-alpha serine/threonine-protein kinase
v-akt murine thymoma viral oncogene homolog 1
The serine threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet derived growth factor. The activation is rapid and specific. In the developing nervous system AKT is a critical mediator of growth factor induced neuronal survival. Survival factors can suppress apoptosis in a transcription independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene (referenced from entrez gene).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Altered expression of BCL2 in cancer Similar to other cell processes, the balance between cell survival and cell death is an important equilibrium that when altered expression of genes can lead to a variety of disease. For example, too little cell death can promote cell overgrowth a... Read full blog post.
AKT1, Scene 1: The Cell Must Go On Akt1 is a serine/threonine-specific protein kinase involved in many cellular signaling pathways. The major function of this kinase is to mediate cell survival, but it also plays key roles in various other cellular functions such as glycogen synthesis... Read full blog post.
EZH2: Epigenetic Regulation Made Easy! Enhancer of Zeste homolog 2 (EZH2) is the methyltransferase enzyme responsible for trimethylating lysine 27 on histone H3 to produce H3K27Me3. EZH2 is a polycomb group protein that is an essential epigenetic regulator that is often found deregulated i... Read full blog post.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
Review this Product
Be the first to review our AKT1 [p Ser473] Antibody and receive a gift card or discount.