Western Blot: Akt1 Antibody (4D6) [NBP2-01724] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using antiAKT1 monoclonal antibody.(1:200)
Immunocytochemistry/ Immunofluorescence: AKT1 Antibody (4D6) [NBP2-01724] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY AKT1.
Immunohistochemistry-Paraffin: AKT1 Antibody (4D6) [NBP2-01724] - Staining of paraffin-embedded Human prostate tissue using anti-AKT1 mouse monoclonal antibody.
Flow Cytometry: AKT1 Antibody (4D6) [NBP2-01724] - Analysis of Jurkat cells, using anti-AKT1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: AKT1 Antibody (4D6) [NBP2-01724] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Western Blot: AKT1 Antibody (4D6) [NBP2-01724] Analysis of extracts (35ug) from 9 different cell lines by using anti-AKT1 monoclonal antibody.
Western Blot: AKT1 Antibody (4D6) [NBP2-01724] - Detection of AKT1 in Human MD-MB-231 cells using NBP2-01724 at a dilution of 1:2000. Photo courtesy of a product review by verified customer.
Immunohistochemistry-Paraffin: AKT1 Antibody (4D6) [NBP2-01724] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-AKT1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: AKT1 Antibody (4D6) [NBP2-01724] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-AKT1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: AKT1 Antibody (4D6) [NBP2-01724] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-AKT1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: AKT1 Antibody (4D6) [NBP2-01724] - Staining of paraffin-embedded Human Kidney tissue using anti-AKT1 mouse monoclonal antibody.
56 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 1 Review rated 5 using NBP2-01724 in the following applications:
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for AKT1 Antibody (OTI4D6)
Protein kinase B
rac protein kinase alpha
RAC-alpha serine/threonine-protein kinase
v-akt murine thymoma viral oncogene homolog 1
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq].
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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