Western Blot: Akt1 Antibody (3A3) [NBP1-51602] - Western blot analysis of extracts from COS cells using AKT1 antibody at dilution of 1:1000. Image from verified customer review.
Immunohistochemistry-Paraffin: Akt1 Antibody (3A3) [NBP1-51602] - IHC analysis of paraffin-embedded human endometrial cancer using AKT1 antibody at dilution of 1:25. Image from verified customer review.
Western Blot: Akt1 Antibody (3A3) [NBP1-51602] - Western blot analysis using AKT1 mouse mAb against NIH/3T3 (1), Hela (2),COS7 (3) and Jurkat (4) cell lysate.
Western Blot: Akt1 Antibody (3A3) [NBP1-51602] - Western blot analysis of k562 cell lysate using AKT1 (NBP1-51602) antibody at 1:500.
Immunohistochemistry-Paraffin: Akt1 Antibody (3A3) [NBP1-51602] - IHC analysis of paraffin-embedded human breast carcinoma using AKT1 antibody at dilution of 1:50. Image from verified customer review.
Hu, Mu, PmSpecies Glossary
WB, ELISA, IHC, IHC-P, IP
This product is unpurified. The exact concentration of antibody is not quantifiable.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Ascitic fluid containing 0.03% sodium azide.
0.03% Sodium Azide
Western Blot 1:500-1:2000
Paraffin section data from customer review. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
56 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 3 Reviews rated 5 using NBP1-51602 in the following applications:
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Alternate Names for Akt1 Antibody (3A3)
Protein kinase B
rac protein kinase alpha
RAC-alpha serine/threonine-protein kinase
v-akt murine thymoma viral oncogene homolog 1
AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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