AKT1 Antibody (3A3)

Images

 
Western Blot: Akt1 Antibody (3A3) [NBP1-51602] - Western blot analysis of extracts from COS cells using AKT1 antibody at dilution of 1:1000. Image from verified customer review.
Immunohistochemistry-Paraffin: Akt1 Antibody (3A3) [NBP1-51602] - IHC analysis of paraffin-embedded human breast carcinoma using AKT1 antibody at dilution of 1:50. Image from verified customer review.
Western Blot: Akt1 Antibody (3A3) [NBP1-51602] - Western blot analysis using AKT1 mouse mAb against NIH/3T3 (1), Hela (2),COS7 (3) and Jurkat (4) cell lysate.
Western Blot: Akt1 Antibody (3A3) [NBP1-51602] - Western blot analysis of k562 cell lysate using AKT1 (NBP1-51602) antibody at 1:500.
Immunohistochemistry-Paraffin: Akt1 Antibody (3A3) [NBP1-51602] - IHC analysis of paraffin-embedded human endometrial cancer using AKT1 antibody at dilution of 1:25. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, PmSpecies Glossary
Applications WB, ELISA, IHC, IHC-P, IP
Clone
3A3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

AKT1 Antibody (3A3) Summary

Immunogen
Purified recombinant fragment of human AKT1 expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
AKT1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:10-1:500
Application Notes
Paraffin section data from customer review.
Theoretical MW
56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 3 Reviews rated 5
using
NBP1-51602 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for AKT1 Antibody (3A3)

  • AKT
  • Akt1
  • EC 2.7.11
  • EC 2.7.11.1
  • PKB alpha
  • PKBMGC99656
  • PRKBA
  • Protein kinase B
  • Proto-oncogene c-Akt
  • rac protein kinase alpha
  • RAC-alpha serine/threonine-protein kinase
  • RAC-alpha
  • RAC-PK-alpha
  • RACPKB-ALPHA
  • v-akt murine thymoma viral oncogene homolog 1

Background

AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AKT1 Antibody (NBP1-51602) (0)

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Reviews for AKT1 Antibody (NBP1-51602) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.
We have 3 reviews tested in 2 application: IHC-P, WB.

Reviews using NBP1-51602:
Filter by Applications
IHC-P
(1)
WB
(2)
All Applications
Filter by Species
Human
(3)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin AKT1 NBP1-51602
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reviewed by:
Anony- mous
IHC-P Human 08/29/2014
View

Summary

ApplicationImmunohistochemistry-Paraffin
SpeciesHuman
Special ApplicationsNBP1-51602 AKT1 antibody detected endogenous level of AKT1 in human breast carcinoma and human endometrial cancer in IHC at dilution of 1:50 and 1:25 respectively.
FileView PDF
Western Blot AKT1 NBP1-51602
Enlarge
reviewed by:
Anony- mous
WB Human 08/29/2014
View

Summary

ApplicationWestern Blot
SpeciesHuman
Special ApplicationsNBP1-51602 AKT1 antibody detected endogenous level of AKT1 in COS cell lysates in WB at dilution of 1:1000.
FileView PDF
Western Blot AKT1 NBP1-51602
Enlarge
reviewed by:
Liyong Zhang
WB Human 08/16/2014
View

Summary

ApplicationWestern Blot
SpeciesHuman
FileView PDF

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for AKT1 Antibody (NBP1-51602). (Showing 1 - 1 of 1 FAQ).

  1. I am looking for a antibody that recognizes human Akt1 but NOT Akt2 or 3, for Western blot analyses. I also want that antibody to recognize Akt1 regardless of its phosphorylated form.
    • At the moment we do not have an AKT1 antibody that definitively does not react with either AKT2 or AKT3.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for AKT1 Antibody (NBP1-51602)

Discover related pathways, diseases and genes to AKT1 Antibody (NBP1-51602). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for AKT1 Antibody (NBP1-51602)

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Blogs on AKT1. Showing 1-10 of 11 blog posts - Show all blog posts.

Using a STAT3 antibody in chromatin immunoprecipitation (ChIP)
Signal transducer and activator of transcription 3 (STAT3) is an important oncogenic transcriptional factor that mediates tumor induced immune suppression.  Specifically, STAT3 transmits signals from cytokines and growth factor receptors in the pla...  Read full blog post.

Altered expression of BCL2 in cancer
Similar to other cell processes, the balance between cell survival and cell death is an important equilibrium that when altered expression of genes can lead to a variety of disease.  For example, too little cell death can promote cell overgrowth a...  Read full blog post.

AKT1 - Regulating cell growth and survival through phosphorylation
AKT1 is a serine/threonine protein kinase with homology to protein kinase A (PKA) and protein kinase C (PKC). AKT1 contains the central kinase domain sandwiched between a pleckstrin homology domain and a regulatory domain (1). AKT1 is regulated by...  Read full blog post.

Akt1 - a central player in cell survival signaling
Akt1 is one of three isoforms of Akt belonging to the AGC family of serine/threonine kinases (Akt1, Akt2, and Akt3). All Akt isoforms contain an N-terminal Plekstrin Homology (PH) domain, a C-terminal regulatory domain, and a central catalytic kin...  Read full blog post.

AKT1, Scene 1: The Cell Must Go On
Akt1 is a serine/threonine-specific protein kinase involved in many cellular signaling pathways. The major function of this kinase is to mediate cell survival, but it also plays key roles in various other cellular functions such as glycogen synthesis...  Read full blog post.

Caspase 9 and Mitochondrial Apoptosis Regulation
Caspase 9 (also termed ICE-LAP6, Mch6, Apaf-3) is a member of cysteine protease family of caspases and is encoded by the CASP9 gene in humans. Caspase-9 is involved in mitochondrial apoptosis pathway and is an initiator caspase. Pro-caspase-9 is activ...  Read full blog post.

Mapping Signal Transduction with mTOR Antibodies
The protein encoded by mTOR (mammalian target of rapamycin), also known as dTOR in Drosophila, belongs to a family of phosphatidylinositol kinase-related kinases. These kinases regulate fundamental processes of cell growth, proliferation, metabolism...  Read full blog post.

EZH2: Epigenetic Regulation Made Easy!
Enhancer of Zeste homolog 2 (EZH2) is the methyltransferase enzyme responsible for trimethylating lysine 27 on histone H3 to produce H3K27Me3. EZH2 is a polycomb group protein that is an essential epigenetic regulator that is often found deregulated i...  Read full blog post.

Antibodies Targeting the mTOR Pathway for Many Diseases
The mammalian target of rapamycin (mTOR) is a downstream effector of the phosphatidylinositol 3-kinase (PI3K)/Akt (protein kinase B) signaling pathway that mediates cell survival and proliferation. It is increasingly apparent that mTOR signaling impac...  Read full blog post.

AKT Antibody Assays: A Complex Area with an Easy Solution
We at Novus Biologicals place a lot of emphasis on the kinase signaling pathways. Kinases, or phosphotransferase enzymes play a key role in phosphorylation signaling. Over 500 human protein kinases have so far been discovered. They play essential role...  Read full blog post.

Showing 1-10 of 11 blog posts - Show all blog posts.

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Recent Reviews

5
5
3
4
0
3
0
2
0
1
0

Anony- mous
08/29/2014
Application: IHC-P
Species: Human

Anony- mous
08/29/2014
Application: WB
Species: Human

Liyong Zhang
08/16/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol AKT1
Entrez