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AK2 Antibody (009)

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Immunocytochemistry/Immunofluorescence: AK2 Antibody (009) [NBP2-90182] - Immunofluorescence staining of AK2 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human AK2 monoclonal antibody (1:60) at 4? overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).
Immunocytochemistry/ Immunofluorescence: AK2 Antibody (009) [NBP2-90182] - Immunofluorescence staining of AK2 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF
Clone
009
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-90182AF350
Alexa Fluor 405
NBP2-90182AF405
Alexa Fluor 488
NBP2-90182AF488
Alexa Fluor 532
NBP2-90182AF532
Alexa Fluor 594
NBP2-90182AF594
Alexa Fluor 647
NBP2-90182AF647
Alexa Fluor 700
NBP2-90182AF700
Alexa Fluor 750
NBP2-90182AF750
Biotin
NBP2-90182B
DyLight 350
NBP2-90182UV
DyLight 405
NBP2-90182V
DyLight 488
NBP2-90182G
DyLight 550
NBP2-90182R
DyLight 594
NBP2-90182DL594
DyLight 650
NBP2-90182C
DyLight 680
NBP2-90182FR
DyLight 755
NBP2-90182IR
FITC
NBP2-90182F
Janelia Fluor 549
NBP2-90182JF549
Janelia Fluor 646
NBP2-90182JF646
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AK2 Antibody (009) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human AK2 (P54819-1; Met1-Ile239).
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
AK2
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:20-1:100

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 um filtered solution in PBS
Preservative
No Preservative
Purity
Protein A purified

Notes

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Alternate Names for AK2 Antibody (009)

  • adenylate kinase 2
  • adenylate kinase 2, mitochondrial
  • adenylate kinase isoenzyme 2, mitochondrial
  • ADK2
  • AK 2
  • ATP-AMP transphosphorylase 2
  • EC 2.7.4
  • EC 2.7.4.3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: ADK Antibody [NBP2-15291] - Mouse tissue extracts (50 ug) was separated by 10 % SDS-PAGE, and the membrane was blotted with Adenosine kinase antibody diluted by 1:1500.
Immunocytochemistry/Immunofluorescence: ADK Antibody [NBP2-15291] - Paraformaldehyde-fixed A431, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: ADK Antibody [NBP2-15291] - Paraffin-embedded mouse kidney. Adenosine kinase antibody dilution: 1:500.
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AK3L1 Antibody
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NBP2-00827
Western Blot: AK3L1 Antibody (3B1) [NBP2-00827] Analysis of extracts (35ug) from 9 different cell lines by using anti-AK3L1 monoclonal antibody.
Immunocytochemistry/Immunofluorescence: AK3L1 Antibody (3B1) [NBP2-00827] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY AK3L1.
Immunohistochemistry-Paraffin: AK3L1 Antibody (3B1) [NBP2-00827] - Staining of paraffin-embedded Human liver tissue using anti-AK3L1 mouse monoclonal antibody.
Species: Hu, Mu, Rt, Ca, Pm, Pm
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AK3 Antibody
(SJB3-36)
NBP1-04261
Western Blot: AK3 Antibody (SJB3-36) [NBP1-04261] - The recombinant protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AK3 antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Recombinant Human AK1 Lane 2. : Recombinant Human AK2 Lane 3. : Recombinant Human AK3
Immunocytochemistry/Immunofluorescence: AK3 Antibody (SJB3-36) [NBP1-04261] - Analysis of AK3 in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AK3 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Immunohistochemistry-Paraffin: AK3 Antibody (SJB3-36) [NBP1-04261] - Human liver tissue was incubated with anti-human AK3 (1:100) for 2 hours at room temperature. Slide was then washed in PBS, and was incubated in avidin biosystem anti-rabbit labeled polymer for 30 min at RT. Enzyme detection was performed with DAB chromo-gen.
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Tenascin C
Antibody (4C8MS)
NB110-68136
Flow (Intracellular): Tenascin C Antibody (4C8MS) [NB110-68136] - Figure A: Intracellular stain performed on U87MG Cells with Tenascin C (4C8MS) antibody NB110-68136 (blue) and a matched isotype control NBP1-97005 (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems].Figure B: U87MG Cells were either untreated (orange) or treated with 3uM Monensin (blue). An intracellular stain was performed with Tenascin C (4C8MS) antibody NB110-68136. Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems].
Western Blot: Tenascin C Antibody (4C8MS) [NB110-68136] - Regulation of Tenascin C expression and its effect on fibrotic responses. Confluent foreskin fibroblasts were incubated with TGF-beta (10 ng ml-1 or indicated concentrations) or Tenascin C (TNC) for 24 or 72 h or indicated periods. Whole-cell lysates, culture media and RNA were examined by western analysis (upper panels) and qPCR (lower panel). Representative immunoblots or qPCR results (means+/-s.e.m. of triplicate determinations). S, secreted; L, lysates. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/doifinder/10.1038/ncomms11703), licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: Tenascin C Antibody (4C8MS) [NB110-68136] - SK-MEL-28 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Tenascin C at 5 ug/ml overnight at 4C and detected with an anti-Mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
(2 Reviews)
(22 Publications)
Species: Hu, Mu, Rt, Fe
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready, Flow-IC
RNF130 Antibody
NBP1-81858
Western Blot: RNF130 Antibody [NBP1-81858] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-478
Immunohistochemistry-Paraffin: RNF130 Antibody [NBP1-81858] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Species: Hu
Applications: WB, IHC, IHC-P
Von Willebrand
Factor Antibody
NB600-586
Dual RNAscope ISH-IHC: Von Willebrand Factor Antibody [NB600-586] - Formalin-fixed paraffin-embedded tissue sections of human metastatic tonsil were probed for vWF mRNA (ACD RNAScope probe, catalog # 5860461; Fast Red chromogen, ACD catalog # 322500). Adjacent tissue section was processed for immunohistochemistry using rabbit polyclonal (Novus catalog # NB600-586) at 1:500 dilution for 1 hour at room temperature followed by incubation with the anti-rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue).
Immunohistochemistry: Von Willebrand Factor Antibody [NB600-586] - Formalin fixed paraffin embedded human tonsil stained with Factor VIII antibody.
Immunohistochemistry-Paraffin: Von Willebrand Factor Antibody [NB600-586] - Staining of formalin-fixed, paraffin-embedded Human Kidney sections with 1:2,000 Rabbit Anti-von Willebrand Factor.
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NBP2-27173
Western Blot: GFI-1 Antibody [NBP2-27173] - Analysis of Zinc finger protein Gfi-1 in human HepG2 cell lysate in the 1) absence and 2) presence of immunizing peptide, and 3) mouse RAW cell lysate using this antibody. goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Immunohistochemistry-Paraffin: GFI-1 Antibody [NBP2-27173] - Analysis of Gfi-1 in formalin-fixed, paraffin-embedded human brain tissue using this antibody at 5 ug/ml.
Species: Hu, Mu, Rt, Bv, Ch
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Glyoxalase I
Antibody (Glo1a)
NBP1-19015
Western Blot: Glyoxalase I Antibody (Glo1a) [NBP1-19015] - Detection of GLO-1 in human cell lines. Lane 1,2 and 3: MG-63 osteosarcoma cell line (positive control), 402-91 liposarcoma cell line and 402-91 liposarcoma cell line resistant to trabectedine. This image was submitted via customer Review.
Immunocytochemistry/Immunofluorescence: Glyoxalase I Antibody (Glo1a) [NBP1-19015] - Immunostaining of human retinal endothelial cells. Immunoreactivity for hGlx-1 was detected in human retinal endothelial cells stained with Glo1a mAb using anti mouse IgG Texas Red as secondary Ab. The cells were counter stained with phalloidin (green) and DAPI (blue), nuclear stain. No reactivity was detected in control cells, stained without Go1a mAb. Figure is a representative of 3 independent experiments. Photo courtesy of Dr. Nagaraj, Case Western Reserve University.
Western Blot: Glyoxalase I Antibody (Glo1a) [NBP1-19015] - HeLa whole cell extracts.
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Adenosine
Deaminase/ADA Antibod
NBP1-87404
Immunohistochemistry-Paraffin: Adenosine Deaminase/ADA Antibody [NBP1-87404] - Staining of human duodenum, small intestine, stomach and testis using Anti-Adenosine Deaminase/ADA antibody NBP1-87404 (A) shows similar protein distribution across tissues to independent antibody NBP1-90361 (B).
Immunohistochemistry-Paraffin: Adenosine Deaminase/ADA Antibody [NBP1-87404] - Analysis in human duodenum and testis tissues using NBP1-87404 antibody. Corresponding Adenosine Deaminase/ADA RNA-seq data are presented for the same tissues.
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Cytochrome c
Antibody (7H8.2C12)
NB100-56503
Western Blot: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Metformin activates apoptotic cell death; Right panel: MCF-7 cells were kept in the presence or absence of metformin for 20 days and then processed to obtain mitochondrial or whole cellular extracts. BAX and CYCS expression levels were determined by Western blot as indicated under Material and Methods. Densitometric analysis of the gels was performed as indicated under Material and Methods. PHB and CDK4 were used as purity and loading controls. Image collected and cropped by CiteAb from the following publication (http://www.mdpi.com/2073-4409/8/1/49), licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - p73 was detected in immersion fixed Hela human cell line using NB100-56503 at 25 ug/ml for 3 hours at room temperature. Cells were stained using the NorthernLights(TM) 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Staining was observed in the cytoplasm and mitochondria.
Immunohistochemistry-Paraffin: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Cytochrome C was detected in immersion fixed paraffin-embedded sections of human heart using anti-human mouse monoclonal antibody (Catalog # NB100-56503) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue).Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.
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PGM1 Antibody
(3B8-H4)
H00005236-M01
Western Blot: PGM1 Antibody (3B8-H4) [H00005236-M01] - Analysis of PGM1 expression in transfected 293T cell line by PGM1 monoclonal antibody (M01), clone 3B8-H4.Lane 1: PGM1 transfected lysate(61.4 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: PGM1 Antibody (3B8-H4) [H00005236-M01] - Analysis of monoclonal antibody to PGM1 on HeLa cell. Antibody concentration 20 ug/ml.
Western Blot: PGM1 Antibody (3B8-H4) [H00005236-M01] - Analysis of PGM1 over-expressed 293 cell line, cotransfected with PGM1 Validated Chimera RNAi ( Cat # H00005236-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PGM1 monoclonal antibody (M01), clone 3B8-H4 (Cat # H00005236-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
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Applications: WB, ELISA, ICC/IF, IP, RNAi, S-ELISA
SMAC/Diablo
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NB500-213
Western Blot: Smac Antibody (78-1-118) [NB500-213] - HeLa whole cell extract lysates.
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Western Blot: Catalase Antibody [NBP2-24916] - Analysis in liver lysate of 1) human 2) mouse and 3) rat at 0.1 ug/ml.
Immunohistochemistry-Paraffin: Catalase Antibody [NBP2-24916] - Staining of human liver tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.
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Neutrophil
Elastase/ELA2 Antibod ...
MAB9167
Western Blot: Neutrophil Elastase/ELA2 Antibody (950334) [MAB9167] - Analysis of human bone marrow tissue lysate with 0.5 ug/ml of hELA-2 Monoclonal Antibody followed by HRP-conjugated Anti-Rabbit IgG Antibody. A specific band was detected for hELA-2 at approximately 25-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry: Neutrophil Elastase/ELA2 Antibody (950334) - Azide Free [MAB9167] - Neutrophil Elastase/ELA2 was detected in immersion fixed THP-1 cell using Neutrophil Elastase/ELA2 Monoclonal Antibody at 8 ug/ml dilution. Cells were stained using Donkey anti-Mouse IgG Secondary Antibody [NL557] (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic.
Immunohistochemistry: Neutrophil Elastase/ELA2 Antibody (950334) [MAB9167] - ELA-2 was detected in immersion fixed paraffin-embedded sections of human lymphoma using ELA-2 Monoclonal Antibody at 5 ug/ml. Tissue was stained using Mouse IgG VisUCyte HRP Polymer Antibody (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neutrophils.
(1 Review)
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AK5 Antibody
(OTI2F9)
NBP2-02978
Western Blot: AK5 Antibody (2F9) [NBP2-02978] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AK5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AK5.
Immunocytochemistry/Immunofluorescence: AK5 Antibody (2F9) [NBP2-02978] Staining of COS7 cells transiently transfected by pCMV6-ENTRY AK5.
Immunohistochemistry-Paraffin: AK5 Antibody (2F9) [NBP2-02978] - Staining of paraffin-embedded Human prostate tissue using anti-AK5 mouse monoclonal antibody.
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
WASP Antibody
NB300-996
Immunocytochemistry/Immunofluorescence: WASP Antibody [NB300-996] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunohistochemistry-Paraffin: WASP Antibody [NB300-996] - Staining of paraffin embedded Human Spleen. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: WASP Antibody [NB300-996] - Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
(1 Publication)
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, PEP-ELISA
HP1 alpha Antibody
(SR44-03)
NBP2-67349
Western Blot: HP1 alpha Antibody (SR44-03) [NBP2-67349] - Analysis of HP1 alpha on different cell lysates using anti-HP1 alpha antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: Hela
Immunocytochemistry/Immunofluorescence: HP1 alpha Antibody (SR44-03) [NBP2-67349] - Staining HP1 alpha in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: HP1 alpha Antibody (SR44-03) [NBP2-67349] - Analysis of paraffin-embedded mouse uterus tissue using anti-HP1 alpha antibody. Counter stained with hematoxylin.
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP

Publications for AK2 Antibody (NBP2-90182) (0)

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Product General Protocols

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ICC/IF Video Protocol

FAQs for AK2 Antibody (NBP2-90182) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-90182AF350
Alexa Fluor 405 NBP2-90182AF405
Alexa Fluor 488 NBP2-90182AF488
Alexa Fluor 532 NBP2-90182AF532
Alexa Fluor 594 NBP2-90182AF594
Alexa Fluor 647 NBP2-90182AF647
Alexa Fluor 700 NBP2-90182AF700
Alexa Fluor 750 NBP2-90182AF750
Biotin NBP2-90182B
DyLight 350 NBP2-90182UV
DyLight 405 NBP2-90182V
DyLight 488 NBP2-90182G
DyLight 550 NBP2-90182R
DyLight 594 NBP2-90182DL594
DyLight 650 NBP2-90182C
DyLight 680 NBP2-90182FR
DyLight 755 NBP2-90182IR
FITC NBP2-90182F
Janelia Fluor 549 NBP2-90182JF549
Janelia Fluor 646 NBP2-90182JF646
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Bioinformatics

Gene Symbol AK2