AK3 Antibody (SJB3-36) - BSA Free

Images

 
Western Blot: AK3 Antibody (SJB3-36) [NBP1-04261] - The recombinant protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AK3 antibody (1:1,000). Proteins were visualized ...read more
Immunocytochemistry/ Immunofluorescence: AK3 Antibody (SJB3-36) [NBP1-04261] - Analysis of AK3 in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AK3 antibody (1:100) with goat ...read more
Immunohistochemistry-Paraffin: AK3 Antibody (SJB3-36) [NBP1-04261] - Human liver tissue was incubated with anti-human AK3 (1:100) for 2 hours at room temperature. Slide was then washed in PBS, and was incubated in ...read more
Flow Cytometry: AK3 Antibody (SJB3-36) [NBP1-04261] - Analysis of AK3 in jurkat cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype ...read more
Western Blot: AK3 Antibody (SJB3-36) [NBP1-04261] - The lysate (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human AK3 antibody (1:1000). Proteins were visualized using a goat ...read more
Western Blot: AK3 Antibody (SJB3-36) [NBP1-04261] - The lysate (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human AK3 antibody (1:1000). Proteins were visualized using a goat ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Clone
SJB3-36
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

AK3 Antibody (SJB3-36) - BSA Free Summary

Immunogen
Recombinant human AK3 (adenylaste kinase isozyme 3) purified from E. coli
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
AK3
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for AK3 Antibody (SJB3-36) - BSA Free

  • Adenylate kinase 3 alpha-like 1
  • adenylate kinase 3 like 1
  • Adenylate kinase 3
  • adenylate kinase 3adenylate kinase 6
  • AK 3
  • AK3
  • AK3L1
  • AK6
  • AKL3L
  • AKL3L1
  • EC 2.7.4
  • EC 2.7.4.10
  • FIX
  • GTP:AMP phosphotransferase, mitochondrial

Background

Adenylate kinase (AK; adenosine triphosphate-adenosine monophosphate [ATP-AMP] phospho-transferase, EC 2.7.4.3) is a ubiquitous monomeric enzyme involved energy metabolism of prokaryotic and eukaryotic cells. Three isozymes (AK1, AK2 and AK3) are characterized in vertebrates. AK1 is present in the cytosol of skeletal muscle, brain, and erythrocyte, while AK2 is localized in the intermembrane space of mitochondria of liver, kidney, spleen and heart. AK3, called GTP:AMP phosphotransferase, exists in the mitochondrial matrix of liver and heart. These isozymes contribute to homeostasis of the adenine nucleotide composition in the cell.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol AK3
Uniprot