AHR Antibody [Unconjugated]

Images

 
Western blot shows lysates of C2C12 mouse myoblast cell line, mouse lung tissue, Hepa 1‑6 mouse hepatoma cell line, Neuro‑2A mouse neuroblastoma cell line (negative control), NIH‑3T3 mouse embryonic ...read more
AHR was detected in immersion fixed C2C12 mouse myoblast cell line using Sheep Anti-Mouse AHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6697) at 10 µg/mL for 3 hours at room temperature. Cells were ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

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AHR Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse AHR
Asn706-Ser805 (Thr758Ala)
Accession # P30561
Specificity
Detects mouse AHR in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
AHR
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF6697 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for AHR Antibody [Unconjugated]

  • Ah receptor
  • AHR
  • AH-receptor
  • aryl hydrocarbon receptor
  • BHLHE76
  • bHLHe76aromatic hydrocarbon receptor
  • Class E basic helix-loop-helix protein 76

Background

AHR (Aryl-hydrocarbon receptor; also bHLHE76) is a 100-105 kDa member of the bHLH/PAS transcription factor family. It is widely expressed and serves many functions. First, it binds multiple xenobiotic chemicals in the cytoplasm. This induces dimerization with ARNT, translocation to the nucleus, and activation of P450 genes such as CYP1A1 and UGT1A6. Second, it appears to block cell cycle progression, possibly via a downregulation of CDK proteins. And third, it blocks apoptosis by interacting with E2F1, thus silencing Tap73 and Apaf1 genes. Mouse AHR precursor is 848 amino acids (aa) in length. It contains a nine aa prosegment, plus an 839 aa mature molecule that contains a DNA binding motif (aa 12-39), a bHLH region (aa 40-80), two PAS domains (aa 116-336) and one PAC segment that stabilizes the PAS domains (aa 342-383). There are multiple alleles for mouse AHR. One 95-97 kDa allele shows a premature truncation after Ser805, while a second 112 kDa allele shows a 41 aa substitution for aa 843-848. Over aa 706-805, mouse AHR shares 87% and 63% aa identity with rat and human AHR, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AHR Antibody (AF6697)(2)

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for AHR Antibody (AF6697)

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Diseases for AHR Antibody (AF6697)

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PTMs for AHR Antibody (AF6697)

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Blogs on AHR.

AHR - A transcription factor regulating immunity and tumorigenesis
The aryl hydrocarbon receptor (AHR) is a ligand activated transcription factor that controls the expression of a diverse set of genes. In the absence of ligand, AHR is retained in the cytoplasm. Upon ligand binding AHR translocates to the nucleus w...  Read full blog post.

Aryl Hydrocarbon Signaling: AIP, AhR, ARNT, BMAL1 and more...
AH receptor-interacting protein (AIP) is a 37 kD immunophilin-like factor found in a variety of tissues with expression levels ranging from high (spleen, thymus, pituitary heart, placenta and skeletal muscle) to low (liver, kidney and lung). It mediat...  Read full blog post.

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Bioinformatics

Gene Symbol AHR
Uniprot