AHR Antibody (RPT9) - BSA Free

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Western Blot: AHR Antibody (RPT9) [NB300-515] - Analysis of Aryl Hydrocarbon Receptor was performed by loading 20 ug of HEK293 lysate overexpressing Aryl Hydrocarbon Receptor (right lane B) or empty vector control (left ...read more
Immunocytochemistry/ Immunofluorescence: AHR Antibody (RPT9) [NB300-515] - Analysis of Aryl Hydrocarbon Receptor using Aryl Hydrocarbon Receptor Monoclonal Antibody (RPT9) shows staining in U251 Cells. Aryl Hydrocarbon ...read more
Flow Cytometry: AHR Antibody (RPT9) [NB300-515] - Analysis of Aryl Hydrocarbon Receptor in NIH/3T3 cells compared to an isotype control (blue).
Western Blot: AHR Antibody (RPT9) [NB300-515] - Analysis of 40 ug of HEK293 lysate overexpressing Aryl Hydrocarbon Receptor (right lane) or empty vector control (left lane) and 10ul of PageRuler Prestained Protein ...read more
Immunocytochemistry/ Immunofluorescence: AHR Antibody (RPT9) [NB300-515] - Analysis of Aryl Hydrocarbon Receptor using Aryl Hydrocarbon Receptor Monoclonal Antibody (RPT9) shows staining in A2058 Cells. Aryl Hydrocarbon ...read more
Immunocytochemistry/ Immunofluorescence: AHR Antibody (RPT9) [NB300-515] - Analysis of Aryl Hydrocarbon Receptor using Aryl Hydrocarbon Receptor Monoclonal Antibody (RPT9) shows staining in Hela Cells. Aryl Hydrocarbon ...read more
Flow Cytometry: AHR Antibody (RPT9) [NB300-515] - Analysis of Aryl Hydrocarbon Receptor in Hela cells compared to an isotype control (blue).
Flow Cytometry: AHR Antibody (RPT9) [NB300-515] - Analysis of Aryl Hydrocarbon Receptor in PC-3 cells compared to an isotype control (blue).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ChIP, ELISA, Flow, GS, ICC/IF, IHC, IHC-Fr, IP
Clone
RPT9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

AHR Antibody (RPT9) - BSA Free Summary

Immunogen
Synthetic peptide corresponding to residues R(12) K R R K P(17) V(22) K P I P A E G I K(31) of mouse AhR.
Specificity
Detects the Aryl Hydrocarbon Receptor (AHR).
Isotype
IgG3 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
AHR
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation 1:100
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:50
  • Gel Super Shift Assays 1:1 - 1:100
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:500 - 1:2000
Application Notes
ELISA use reported in the literature (PMID: 10413464). IP: A single ~95 kDa protein representing AHR from Hepa 1 cytosol. IHC: Staining of AHR in rat liver results in strong cytoplasmic and some nuclear staining.
Control
AHR Overexpression Lysate
Publications
Read Publications using
NB300-515 in the following applications:

  • 1 publication
  • WB
    2 publications

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for AHR Antibody (RPT9) - BSA Free

  • Ah receptor
  • AHR
  • AH-receptor
  • aryl hydrocarbon receptor
  • BHLHE76
  • bHLHe76aromatic hydrocarbon receptor
  • Class E basic helix-loop-helix protein 76

Background

The aryl hydrocarbon receptor (AhR), also known as the dioxin receptor, is a ligand-activated helix/loop/helix transcription factor found in a variety of vertebrate species. The known ligands for AhR are foreign planar aromatic compounds, such as polycyclic aromatic compounds and halogenated aromatic compounds such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Unlike the steroid/thyroid hormone receptors, there is no known physiological ligand for the Ah Receptor. Studies indicate that in non-ligand activated cells, AhR is found complexed with HSP90 predominantly in the cytoplasm. Upon binding to an agonist, the ligand-activated AhR is believed to transform to a nuclear, DNA binding form. This transformation process appears to involve dissociation of HSP90 from AhR followed by formation of a heterodimer with AhR nuclear translocator protein (Arnt). The AhR-ligand complex appears to initiate gene transcription of cytochrome P450 1A1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AHR Antibody (NB300-515)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ChIP, WB.


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ChIP
(1)
WB
(2)
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Filter By Species
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Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for AHR Antibody (NB300-515). (Showing 1 - 1 of 1 FAQs).

  1. This clone (RPT9) has been discontinued by other vendors. The current lead time on the Novus website is 2-4 business days. May I know if this is guaranteed, or will this clone also be discontinued by Novus?
    • We have not had any recall from our licensee for this clone, and as of now, we can still provide this product. This may change in the future depending on licensing agreements, but we do not have any intentions of discontinuing this product at this time.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for AHR Antibody (NB300-515)

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Blogs on AHR.

AHR - A transcription factor regulating immunity and tumorigenesis
The aryl hydrocarbon receptor (AHR) is a ligand activated transcription factor that controls the expression of a diverse set of genes. In the absence of ligand, AHR is retained in the cytoplasm. Upon ligand binding AHR translocates to the nucleus w...  Read full blog post.

Aryl Hydrocarbon Signaling: AIP, AhR, ARNT, BMAL1 and more...
AH receptor-interacting protein (AIP) is a 37 kD immunophilin-like factor found in a variety of tissues with expression levels ranging from high (spleen, thymus, pituitary heart, placenta and skeletal muscle) to low (liver, kidney and lung). It mediat...  Read full blog post.

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Bioinformatics

Gene Symbol AHR
Entrez
Uniprot