Ago2/eIF2C2 Antibody (2E12-1C9) - Azide and BSA Free

Western Blot: Ago2/eIF2C2 Antibody (2E12-1C9) [H00027161-M01] - AGO2 contributed predominately to silencing activity in both regions. The relative AGO protein levels were detected by western blot 56 hours post siAgo transfection, at the time point when Ago2/eIF2C2 ablated cells were harvested to evaluate luciferase activities. GAPDH was included as loading control. The intensity of protein bands was quantified by ImageJ software (NIH, USA). All experiments were performed at least twice. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0049309) licensed under a CC-BY license.

Western Blot: Ago2/eIF2C2 Antibody (2E12-1C9) [H00027161-M01] - The impact of different HAd infections on RNAi/miRNA-pathway proteins.HAd infections do not affect RNAi/miRNA-pathway protein levels. Western blot analysis on the same protein samples as in panel A was used to monitor the levels of RNAi/miRNA-pathway proteins Exportin 5, Dicer, TRBP and Ago2/eIF2C2. Detection of the Lamin B protein served as a loading control. Letter

Immunocytochemistry/Immunofluorescence: Ago2/eIF2C2 Antibody (2E12-1C9) [H00027161-M01] - Analysis of monoclonal antibody to EIF2C2 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody (2E12-1C9) [H00027161-M01] - Analysis of monoclonal antibody to EIF2C2 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml.

Western Blot: Ago2/eIF2C2 Antibody (2E12-1C9) [H00027161-M01] - Analysis of EIF2C2 over-expressed 293 cell line, cotransfected with EIF2C2 Validated Chimera RNAi ( Cat # H00027161-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with EIF2C2 monoclonal antibody (M01), clone 2E12-1C9 (Cat # H00027161-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

ELISA: Ago2/eIF2C2 Antibody (2E12-1C9) [H00027161-M01] - Detection limit for recombinant GST tagged EIF2C2 is 0.3 ng/ml as a capture antibody.

Western Blot: Ago2/eIF2C2 Antibody (2E12-1C9) [H00027161-M01] - The impact of different HAd infections on RNAi/miRNA-pathway proteins.(A) Efficiency of different HAd infections. HeLa cells were infected with the indicated viruses, followed by a 35S-methionine pulse labeling after 24 & 48 hpi. Total protein lysates were separated on an SDS-PAGE & protein synthesis visualized by autoradiography. Accumulation of late viral hexon protein is indicated by an arrow. (B) HAd infections do not affect RNAi/miRNA-pathway protein levels. Western blot analysis on the same protein samples as in panel A was used to monitor the levels of RNAi/miRNA-pathway proteins Exportin 5, Dicer, TRBP & Ago2. Detection of the Lamin B protein served as a loading control. Letter “M” denotes mock, non-infected samples. The different panels were repeated at least two times. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25144466), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Ago2/eIF2C2 Antibody (2E12-1C9) [H00027161-M01] - AGO2 contributed predominately to silencing activity in both regions.(A) The relative AGO mRNA levels were measured by quantitative RT-PCR 24 hours post siAgo transfection. Results were average values of assays in triplicates, & all experiments were repeated three times. (B) The relative AGO protein levels were detected by western blot 56 hours post siAgo transfection, at the time point when AGO ablated cells were harvested to evaluate luciferase activities. GAPDH was included as loading control. The intensity of protein bands was quantified by ImageJ software (NIH, USA). All experiments were performed at least twice. (C) The normalized silencing efficacies of siR-04 on perfectly matched target in CDS versus 3′-UTR after AGOs ablation. Silencing of AGO expression was carried out by gene-specific siRNA assessed in the previous study [9], & subsequently, influence of the gene silencing on perfectly-match tolerance was evaluated by reporter system. All data were normalized to mock. (D) The normalized silencing efficacies of siR-04 on single-nucleotide mismatched target sites in CDS versus 3′-UTR at the indicated positions (4C, 10U, 12G, 17A) after AGOs ablation. The target site location & siRNA:mRNA match pattern were given under the x-axis. Error bars represented SD. Data were average values of assays in triplicates, & all experiments were repeated at least twice. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23145149), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunoprecipitation: Ago2/eIF2C2 Antibody (2E12-1C9) [H00027161-M01] - Snail1 is a target of miR‐30c. (A) Sequence alignment between miR‐30c & the 3′‐UTR of Snail1 among several species. (B) Ago2 protein levels in co‐immunoprecipitated products detected by Western blot. IgGHC, IgG heavy chain; IgGLC, IgG light chain. (C) Relative expression of Snail1 in the whole RNA (left) & RNA of the nonspecific IgG or anti‐Ago2 co‐IP (right) from the HG‐treated HK2 cell lysates. #P < 0.05 vs. miR‐con + input, *P < 0.05 vs. miR‐con + IgG IP. (D) Schematic diagram of the luciferase reporter plasmids of pMIR‐Snail1 3′‐UTR & pMIR‐Snail1 3′‐UTR mut, & the potential target site of miR‐30c on the 3′‐UTR of Snail1. (E) Regulation of miR‐30c on 3′‐UTR of Snail1 in HEK293 cells by luciferase reporter assay. *P < 0.05 vs. Snail1 3′‐UTR + miR‐con. (F) Snail1 protein levels of HK2 cells with different treatments detected by Western blot. *P < 0.05 vs. NG, #P < 0.05 vs. HG + miR‐con, &P < 0.05 vs. HG + inhibitor‐con. (G) Snail1 protein levels of renal cortex detected by Western blot. *P < 0.05 vs. C57BL/Ks. #P < 0.05 vs. db/db control. (H) Stability curves of Snail1 mRNA in HG‐treated HK2 cells after transfection of miR‐30c mimics (left) or inhibitor (right). (I) The relative abundance of individual mRNA in each fraction was presented as the percentage of the total fraction following miR‐con (left) or miR‐30c (right) transfection. (J) The association of the Snail1 mRNA with putative polysome fractions (fraction 12 & fraction 13) after miR‐30c mimics transfection. Data are representative of three experiments. Data are expressed as mean ± SEM, n ≥ 3. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28127848), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Po, Pm, Xp
• Applications: WB, ELISA, ICC/IF, IHC, IP, WB, ChIP, KD
• Clone: 2E12-1C9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Ago2/eIF2C2 Antibody (2E12-1C9) - Azide and BSA Free Summary

• Immunogen: EIF2C2 (AAH07633.1, 483 a.a ~ 859 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPIQGQPCFCKYAQGADSVEPMFRHLKNTYAGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTSGQSNGRDHQALAKAVQVHQDTLRTMYFA
• Specificity: EIF2C2 - eukaryotic translation initiation factor 2C, 2
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: AGO2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 1:10-1:500
  • ELISA 1:100-1:2000
  • Immunoblotting
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation
  • Knockdown Validated
  • Western Blot 1:500
• Application Notes: Antibody reactive against recombinant protein and cell lysate for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), RNAi validation and ELISA and Sandwich ELISA. Chromatin Immunoprecipitation was reported in scientific literature. Use in immunprecipitation reported in scientific literature (PMID 24658750). Use in immunoblotting reported in scientific literature (PMID: 31393866).
• Control:

  Human Bladder Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 26858302). Mouse reactivity reported in scientific literature (PMID: 28127848)

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Ago2/eIF2C2 Antibody (2E12-1C9) - Azide and BSA Free

• AGO2argonaute 2
• argonaute2
• EC 3.1.26.n1
• EC 3.1.26.n2
• eIF2C 2
• eIF-2C 2
• Eukaryotic translation initiation factor 2C 2
• eukaryotic translation initiation factor 2C, 2
• hAgo2
• MGC3183
• PAZ Piwi domain protein
• PPD
• protein argonaute-2
• Protein slicer
• Q10

Background

This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ago2/eIF2C2 Antibody (H00027161-M01)(95)

Publications using H00027161-M01

• Lin MC, Kuo WH, Chen SY et Al. Ago2/CAV1 interaction potentiates metastasis via controlling Ago2 localization and miRNA action EMBO Rep 2024-04-22 [PMID: 38649663]
• Seungjae L, David J, Sid S et al. Promiscuous splicing-derived hairpins are dominant substrates of tailing-mediated defense of miRNA biogenesis in mammals. Cell Rep. 2023-02-16 [PMID: 36800291]
• Samantha M, Nicolai K, Sayaka D et al. Massively parallel identification of mRNA localization elements in primary cortical neurons. Nat Neurosci. 2023-01-16 [PMID: 36646877]
• Vladimir L, Karel D, Louis J et al. Identification of a Novel HBV Encoded miRNA Using Next Generation Sequencing Viruses. 2022-06-05 [PMID: 35746694]
• Ye Q, Li D, Siwen Z et al. A plant immune protein enables broad antitumor response by rescuing microRNA deficiency. Cell. 2022-05-26 [PMID: 35623329]
• Liu Z, Du J, Ren J et al. miR-183-96-182 clusters alleviated ox-LDL-induced vascular endothelial cell apoptosis in vitro by targeting FOXO1 RSC Adv 2022-05-13 [PMID: 35547044]
• S Chatterjee, I Mukherjee, S Bhattachar, M Bose, S Chakrabart, SN Bhattachar Target-Dependent Coordinated Biogenesis of Secondary MicroRNAs by miR-146a Balances Macrophage Activation Processes Molecular and Cellular Biology, 2022-03-21;0(0):e0045221. 2022-03-21 [PMID: 35311564]
• Lotteke Z, Yichen L, Annika S et al. The Role of the MYC/miR-150/MYB/ZDHHC11 Network in Hodgkin Lymphoma and Diffuse Large B-Cell Lymphoma. Genes (Basel). 2022-01-24 [PMID: 35205272]
• Wang S, Yang Y, Suen A Et al. Role of extracellular microRNA-146a-5p in host innate immunity and bacterial sepsis iScience 2021-12-01 [PMID: 34877498]
• Erin N, Ruth R, Koon-Kiu Y et al. miR-424/503 modulates Wnt/?-catenin signaling in the mammary epithelium by targeting LRP6. EMBO Rep. 2021-10-11 [PMID: 34633138]
• Bharti N, Arpita S, Sumantra C et al. Distinct temporal expression of GW182 (TNRC6A) in neurons regulates dendritic arborization. J Cell Sci. 2021-08-24 [PMID: 34328181]
• De D, Bhattacharyya SN., et Al. Amyloid-? oligomers block lysosomal targeting of miRNPs to prevent miRNP recycling and target repression in glial cells J Cell Sci 2021-07-12 [PMID: 34096603]
• Dipayan D, Suvendra B Amyloid Beta oligomers prevents Lysosomal targeting of miRNP to stop its recycling and target repression in glial cells. J Cell Sci. 2021-06-07 [PMID: 34002227]
• Xinxin Z, Jie Z, Yinming L et al. TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS. Nat Struct Mol Biol. 2021-01-04 [PMID: 33398173]
• Fubiao N, Agnieszka D, Jasper K et al. MiR-378a-3p Is Critical for Burkitt Lymphoma Cell Growth. Cancers (Basel). 2020-11-27 [PMID: 33261009]
• Jiabing Z, Huizhen L, Beibei D et al. The nuclear and cytoplasmic roles of miR-320 in non-alcoholic fatty liver disease. Aging (Albany NY). 2020-11-07 [PMID: 33186123]
• Qin M, Atsuko M, Brenda H et al. Oligodendrocyte-specific Argonaute profiling identifies microRNAs associated with experimental autoimmune encephalomyelitis. J Neuroinflammation. 2020-10-12 [PMID: 33046105]
• Yuying L, Chenghui Y, Jiahui F et al. MiR-221-3p targets Hif-1? to inhibit angiogenesis in heart failure. Lab Invest. 2020-09-01 [PMID: 32873879]
• Aida M, Stefano L, Eshita S et al. High-Throughput Identification of MiR-145 Targets in Human Articular Chondrocytes. Life (Basel). 2020-05-11 [PMID: 32403239]
• Monika D, Bronislawa S, Roman J et al. hsa-miR-20b-5p and hsa-miR-363-3p Affect Expression of PTEN and BIM Tumor Suppressor Genes and Modulate Survival of T-ALL Cells In Vitro. Cells. 2020-05-05 [PMID: 32380791]
• Bose M, Chatterjee S, Chakrabarty Y et al. Retrograde trafficking of Argonaute 2 acts as a rate-limiting step for de novo miRNP formation on endoplasmic reticulum-attached polysomes in mammalian cells. Life Sci Alliance. 2020-02-03 [PMID: 32015087]
• Li H, Zhan J, Zhao Y et al. Identification of ncRNA-Mediated Functions of Nucleus-Localized miR-320 in Cardiomyocytes. Mol Ther Nucleic Acids. 2019-11-18 [PMID: 31837603]
• Fan J, Zhang X, Nie X et al. Nuclear miR-665 aggravates heart failure via suppressing phosphatase and tensin homolog transcription. Sci China Life Sci. 2019-10-28 [PMID: 31664601]
• Park J, Seo J, Ahn N et al. UPF1/SMG7-dependent microRNA-mediated gene regulation. Nat Commun. 2019-09-13 [PMID: 31519907]
• Kaczmarczyk L, Bansal V, Rajput A et al. Tagger-A Swiss army knife for multiomics to dissect cell type-specific mechanisms of gene expression in mice PLoS Biol. 2019-08-01 [PMID: 31393866] (IB, Mouse)
• Kute P, Ramakrishna S, Neelagandan N et al. NMDAR mediated translation at the synapse is regulated by MOV10 and FMRP. Mol Brain. 2019-07-10 [PMID: 31291981]
• Tiwari D, Brager D, Rymer J et al. MicroRNA inhibition upregulates hippocampal A-type potassium current and reduces seizure frequency in a mouse model of epilepsy. Neurobiol Dis. 2019-06-15 [PMID: 31212067]
• He M, Wang J, Yin Z et al. MiR-320a induces diabetic nephropathy via inhibiting MafB Aging (Albany NY) 2019-05-17 [PMID: 31102503] (Chemotaxis, Mouse)
• Han X, Li Q, Liu C et al. Overexpression miR-24-3p repressed Bim expression to confer tamoxifen resistance in breast cancer. J Cell Biochem. 2019-04-18 [PMID: 31001849]
• Zhang K, Zhang X, Cai Z et al. A novel class of microRNA-recognition elements that function only within open reading frames. Nat Struct Mol Biol 2018-10-08 [PMID: 30297778]
• Daga N, Eicher S, Kannan A et al. Growth-restricting effects of siRNA transfections: a largely deterministic combination of off-target binding and hybridization-independent competition. Nucleic Acids Res. 2018-09-12 [PMID: 30215772] (Human)
• Nie X, Fan J, Li H et al. miR-217 Promotes Cardiac Hypertrophy and Dysfunction by Targeting PTEN. Mol Ther Nucleic Acids. 2018-06-17 [PMID: 30195764]
• Dai B, Li H, Fan J et al. MiR-21 protected against diabetic cardiomyopathy induced diastolic dysfunction by targeting gelsolin. Cardiovasc Diabetol 2018-09-04 [PMID: 30180843]
• Dzikiewicz-Krawczyk A, Diepstra A, Rutgers B et al. AGO2 RNA Immunoprecipitation Reveals Distinct miRNA Targetomes of Primary Burkitt Lymphoma Tumors and Normal B Cells. Am J Pathol 2018-02-16 [PMID: 29458013]
• Song X, Wang X, Ma Y et al. Site-Specific Modification Using the 2'-Methoxyethyl Group Improves the Specificity and Activity of siRNAs. Mol Ther Nucleic Acids 2017-10-07 [PMID: 29246303]
• Patranabis S, Bhattacharyya SN. P-body-induced inactivation of let-7a miRNP prevents the death of growth factor-deprived neuronal cells FASEB J. 2017-11-22 [PMID: 29167236] (WB, Rat)
• Ong J, Timens W, Rajendran V et al. Identification of transforming growth factor-beta-regulated microRNAs and the microRNA-targetomes in primary lung fibroblasts. PLoS One 2017-09-14 [PMID: 28910321]
• Elkayam E, Faehnle CR, Morales M et al. Multivalent Recruitment of Human Argonaute by GW182. Mol Cell 2017-08-03 [PMID: 28781232]
• Curtale G, Renzi TA, Drufuca L et al. Glucocorticoids downregulate TLR4 signaling activity via its direct targeting by miR-511-5p. Eur J Immunol 2017-08-04 [PMID: 28776644]
• Yuan Y, Kluiver J, Koerts J et al. miR-24-3p Is Overexpressed in Hodgkin Lymphoma and Protects Hodgkin and Reed-Sternberg Cells from Apoptosis. Am J Pathol 2017-04-19 [PMID: 28432871]
• Huang JH, Ku WC, Chen YC et al. Dual mechanisms regulate the nucleocytoplasmic localization of human DDX6. Sci Rep 2017-02-20 [PMID: 28216671]
• Zhao Y, Yin Z, Li H et al. MiR-30c protects diabetic nephropathy by suppressing epithelial-to-mesenchymal transition in db/db mice Aging Cell 2017-01-27 [PMID: 28127848] (IP, Mouse)
• Bose M, Barman B, Goswami A, Bhattacharyya SN. Spatio-temporal uncoupling of miRNA-mediated translational repression and target RNA degradation controls miRNP recycling in mammalian cells. Mol Cell Biol 2016-11-28 [PMID: 27895152]
• Wang WT, Ye H, Wei PP et al. LncRNAs H19 and HULC, activated by oxidative stress, promote cell migration and invasion in cholangiocarcinoma through a ceRNA manner. J Hematol Oncol 2016-11-03 [PMID: 27809873]
• Mantel PY, Hjelmqvist D, Walch M et al. Infected erythrocyte-derived extracellular vesicles alter vascular function via regulatory Ago2-miRNA complexes in malaria. Nat Commun 2016-10-10 [PMID: 27721445]
• He M. miRNA Tagging and Affinity-purification (miRAP). Bio Protoc 2012-10-05 [PMID: 27525292]
• Bose M, Bhattacharyya SN. Target-dependent biogenesis of cognate microRNAs in human cells Nat Commun 2016-07-24 [PMID: 27448149] (WB)
• Patranabis S, Bhattacharyya SN. Phosphorylation of Ago2 and Subsequent Inactivation of let-7a miRNPs Control Differentiation of Mammalian Sympathetic Neurons. Mol. Cell. Biol. 2016-02-08 [PMID: 26858302] (WB, Rat)
• Seidl CI, Martinez-Sanchez A, Murphy CL. Derepression of miRNA-138 contributes to loss of the human articular chondrocyte phenotype. Arthritis Rheumatol. 2015-09-11 [PMID: 26359943]
• Xiao S, Wang X, Ni H et al. MiR-24-3p Promotes Porcine Reproductive and Respiratory Syndrome Virus Replication through Suppression of Heme Oxygenase-1 Expression. J Virol. 2015-02-04 [PMID: 25653454]
• Ghosh S, Bose M, Ray A et al. Polysome arrest restricts miRNA turnover by preventing exosomal export of miRNA in growth-retarded mammalian cells. Mol Biol Cell. 2015-03-15 [PMID: 25609084] (WB, Human)
• Llobet-Navas D, Rodriguez-Barrueco R, de la Iglesia-Vicente J et al. The microRNA 424/503 cluster reduces CDC25A expression during cell cycle arrest imposed by transforming growth factor beta in mammary epithelial cells. Mol Cell Biol. 2014-12-01 [PMID: 25266660] (IP)
• Yan Y, Eipper BA, Mains RE. Kalirin-9 and Kalirin-12 Play Essential Roles in Dendritic Outgrowth and Branching Cereb. Cortex 2014-08-21 [PMID: 25146373]
• Kamel W, Segerman B, Punga T, Akusjarvi G. Small RNA Sequence Analysis of Adenovirus VA RNA-Derived MiRNAs Reveals an Unexpected Serotype-Specific Difference in Structure and Abundance. PLoS One. 2014-08-21 [PMID: 25144466]
• Zhang X, Zuo X, Yang B et al. MicroRNA Directly Enhances Mitochondrial Translation during Muscle Differentiation. Cell. 2014-07-31 [PMID: 25083871]
• Le Guillou Sandrine, Marthey Sylvain, Laloe Denis et al. Characterisation and comparison of lactating mouse and bovine mammary gland miRNomes. PLoS One. 2014-03-21 [PMID: 24658750] (IP)
• Zhang Q, Guo XK, Gao L et al. MicroRNA-23 inhibits PRRSV replication by directly targeting PRRSV RNA and possibly by upregulating type I interferons. Virology. 2014-01-01 [PMID: 24503081]
• Pai B, Siripornmongcolchai T, Berentsen B et al. NMDA receptor-dependent regulation of miRNA expression and association with Argonaute during LTP in vivo. Front Cell Neurosci. 2014-01-13 [PMID: 24454279] (IP)
• Fan M, Krutilina R, Sun J et al. Comprehensive Analysis of MicroRNA (miRNA) Targets in Breast Cancer Cells. J Biol Chem. 2013-08-06 [PMID: 23921383]
• Sasaki Y, Gross C, Xing L et al. Identification of axon-enriched microRNAs localized in growth cones of cortical neurons. Dev Neurobiol. 2013-07-29 [PMID: 23897634]
• Karginov FV, Hannon GJ. Remodeling of Ago2-mRNA interactions upon cellular stress reflects miRNA complementarity and correlates with altered translation rates. Genes Dev. 2013-07-03 [PMID: 23824327]
• Curtale G, Mirolo M, Renzi TA et al. Negative regulation of Toll-like receptor 4 signaling by IL-10-dependent microRNA-146b. Proc Natl Acad Sci U S A. 2013-06-24 [PMID: 23798430]
• Zheng J, Zhang L, Zhang J et al. Single modification at position 14 of siRNA strand abolishes its gene-silencing activity by decreasing both RISC loading and target degradation. FASEB J. 2013-06-14 [PMID: 23771927]
• Laffont B, Corduan A, Ple H et al. Activated platelets can deliver mRNA regulatory Ago2{middle dot}microRNA complexes to endothelial cells via microparticles. Blood. 2013-05-07 [PMID: 23652806]
• Wei Y, Nazari-Jahantigh M, Chan L et al. The microRNA-342-5p Fosters Inflammatory Macrophage Activation Through an Akt1- and microRNA-155-Dependent Pathway During Atherosclerosis. Circulation. 2013-03-19 [PMID: 23513069]
• Nakanishi H, Taccioli C, Palatini J et al. Loss of miR-125b-1 contributes to head and neck cancer development by dysregulating TACSTD2 and MAPK pathway. Oncogene. 2013-02-18 [PMID: 23416980]
• Hoffman AE, Liu R, Fu A et al. Targetome Profiling, Pathway Analysis and Genetic Association Study Implicate miR-202 in Lymphomagenesis. Cancer Epidemiol Biomarkers Prev 2013-03-01 [PMID: 23334589] (Chemotaxis, Human)
• Xue Y, Ouyang K, Huang J et al. Direct Conversion of Fibroblasts to Neurons by Reprogramming PTB-Regulated MicroRNA Circuits. Cell. 2012-11-09 [PMID: 23313552]
• Guo XK, Zhang Q, Gao L et al. Additional expression of microRNA-181 inhibits Porcine Reproductive and Respiratory Syndrome Virus replication and its implications for controlling virus infection. J Virol. 2012-11-14 [PMID: 23152505]
• Wei N, Zhang L, Huang H et al. siRNA Has Greatly Elevated Mismatch Tolerance at 3'-UTR Sites. PLoS One. 2012-11-08 [PMID: 23145149]
• Loedige I, Gaidatzis D, Sack R et al. The mammalian TRIM-NHL protein TRIM71/LIN-41 is a repressor of mRNA function. Nucleic Acids Res. 2012-11-03 [PMID: 23125361]
• Nazari-Jahantigh M, Wei Y, Noels H et al. MicroRNA-155 promotes atherosclerosis by repressing Bcl6 in macrophages. J Clin Invest. 2012-10-08 [PMID: 23041630]
• Tiedje C, Ronkina N, Tehrani M et al. The p38/MK2-Driven Exchange between Tristetraprolin and HuR Regulates AU-Rich Element-Dependent Translation. PLoS Genet. 2012-09-27 [PMID: 23028373]
• Reed JC, Molter B, Geary CD et al. HIV-1 Gag co-opts a cellular complex containing DDX6, a helicase that facilitates capsid assembly. J Cell Biol. 2012-07-30 [PMID: 22851315]
• Kluiver J, Slezak-Prochazka I, Smigielska-Czepiel K et al. Generation of miRNA sponge constructs. Methods. 2012-07-23 [PMID: 22836127]
• Dolezalova D, Mraz M, Barta T et al. MicroRNAs Regulate p21(Waf1/cip1) Protein Expression and the DNA Damage Response in Human Embryonic Stem Cells. Stem Cells. 2012-04-17 [PMID: 22511267]
• Banez-Coronel M, Porta S, Kagerbauer B et al. A Pathogenic Mechanism in Huntington's Disease Involves Small CAG-Repeated RNAs with Neurotoxic Activity. PLoS Genet. 2012-02-23 [PMID: 22383888]
• Chiang K, Sung TL, Rice AP. Regulation of cyclin T1 and HIV-1 Replication by microRNAs in resting CD4+ T lymphocytes. J Virol. 2012-03-01 [PMID: 22205749] (IP)
• Martinez-Sanchez A, Dudek KA, Murphy CL. Regulation of human chondrocyte function through direct inhibition of cartilage master-regulator SOX9 by miRNA-145. J Biol Chem. 2011-11-18 [PMID: 22102413]
• Cook D, Del Rayo Sanchez-Carbente M, Lachance C et al. Fragile X Related Protein 1 Clusters with Ribosomes and Messenger RNAs at a Subset of Dendritic Spines in the Mouse Hippocampus. PLoS One. 2011-10-11 [PMID: 22022532]
• Turchinovich A, Weiz L, Langheinz A, Burwinkel B. Characterization of extracellular circulating microRNA. Nucleic Acids Res. 2011-05-24 [PMID: 21609964]
• Lund E, Sheets MD, Imboden SB, Dahlberg JE. Limiting Ago protein restricts RNAi and microRNA biogenesis during early development in Xenopus laevis. Genes Dev. 2011-05-16 [PMID: 21576259]
• Glorian V, Maillot G, Poles S et al. HuR-dependent loading of miRNA RISC to the mRNA encoding the Ras-related small GTPase RhoB controls its translation during UV-induced apoptosis. Cell Death Differ. 2011-04-29 [PMID: 21527938]
• Rawlings RA, Krishnan V, Walter NG. et al. Viral RNAi Suppressor Reversibly Binds siRNA to Outcompete Dicer and RISC via Multiple Turnover. J Mol Biol 2011-02-24 [PMID: 21354178]
• Wang Y, Lacroix G, Haines J et al. The QKI-6 RNA binding protein localizes with the MBP mRNAs in stress granules of glial cells. PLoS One;5(9). 2010-09-17 [PMID: 20862255]
• Cheloufi S, Dos Santos CO, Chong MM et al. A dicer-independent miRNA biogenesis pathway that requires Ago catalysis. Nature;465. 2010-06-03 [PMID: 20424607]
• Flemr M, Ma J, Schultz RM, Svoboda P. P-Body Loss Is Concomitant with Formation of a Messenger RNA Storage Domain in Mouse Oocytes. Biol Reprod. 2010-01-14 [PMID: 20075394]
• Lin JC, Tarn WY. RNA-binding motif protein 4 translocates to cytoplasmic granules and suppresses translation via argonaute2 during muscle cell differentiation. J Biol Chem 284(50):34658-65. 2009-12-11 [PMID: 19801630]
• Tan LP, Seinen E, Duns G et al. A high throughput experimental approach to identify miRNA targets in human cells. Nucleic Acids Res. 2009-09-04 [PMID: 19734348]
• Landry P, Plante I, Ouellet DL et al. Existence of a microRNA pathway in anucleate platelets. Nat Struct Mol Biol 16(9):961-6. 2009-09-01 [PMID: 19668211]
• Zeitelhofer M, Karra D, Macchi P et al. Dynamic Interaction between P-Bodies Transport Ribonucleoprotein Particles in Dendrites of Mature Hippocampal Neurons. J Neurosci;28(30):7555-62. 2008-07-23 [PMID: 18650333]
• Zeng Y, Sankala H, Zhang X, Graves PR Phosphorylation of Argonaute 2 at serine-387 facilitates its localization to processing bodies. Biochem J 2008-08-01 [PMID: 18476811] (WB)
• Alisch RS, Jin P, Epstein M et al. Argonaute2 Is Essential for Mammalian Gastrulation Proper Mesoderm Formation. PLoS Genet;3(12):e227. 2007-12-28 [PMID: 18166081]
• Vickers TA, Lima WF, Nichols JG et al. Reduced levels of Ago2 expression result in increased siRNA competition in mammalian cells. Nucleic Acids Res;35(19):6598-610. 2007-01-01 [PMID: 17905815]
• Krol J, Fiszer A, Mykowska A et al. Ribonuclease Dicer Cleaves Triplet Repeat Hairpins into Shorter Repeats that Silence Specific Targets. Mol Cell;25(4):575-86. 2007-02-23 [PMID: 17317629]

Reviews for Ago2/eIF2C2 Antibody (H00027161-M01) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Mouse, Other.

reviewed by: Souvik Ghosh WB Mouse 04/27/2018

Summary

• Application: Western Blot
• Sample Tested: mouse cell
• Species: Mouse
• Lot: Not Available

Comments

• Comments: NIH3T3 cells were FACS sorted based on marker gene expression.Cell lysates were compared for Ago2 expression levels

reviewed by: Verified Customer WB Other 04/26/2010

Summary

• Application: Western Blot
• Sample Tested: HeLa 4
• Species: Other
• Lot: Lot B 3

FAQs for Ago2/eIF2C2 Antibody (H00027161-M01). (Showing 1 - 1 of 1 FAQs).

1. I ordered the H00027161-M01 antibody for detecting AGO2. I am using it for both IPs and westerns. The product sheet says the AGO2 should run at ~42 kDa, but online, in HL-60 cells, AGO2 runs at ~80-90 kDa, which is more of what I would expect. Can you please explain this discrepancy?
   • Ago2 should run at around 80-90 kDa. Abnova generated their antibody against a 42 kDa partial recombinant protein. The image you see showing Ago2 at 42 kDa is from them probing the partial recombinant protein with H00027161-M01.

Bioinformatics

• Gene Symbol: AGO2
• Entrez:
  • 27161(Human)
• OMIM:
  • 606229(Human)
• Uniprot:
  • AAH07633(Human)