Human Bladder Whole Tissue Lysate (Adult Whole Normal)

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SDS-Page: Human Bladder Whole Tissue Lysate (Whole Normal) [NB820-59173] - Protein lysate is verified on SDS-PAGE analysis as a consistent pattern.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Concentration
5.0 mg/ml

Order Details

Human Bladder Whole Tissue Lysate (Adult Whole Normal) Summary

Specificity
Total Protein - Human Adult Normal Tissue: Bladder

Applications/Dilutions

Application Notes
Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression
Positive Control
14-3-3 sigma/Stratifin Antibody (NB100-81672)
Uroplakin Ib Antibody (NBP1-80656)
Uroplakin III Antibody (NBP2-12482)

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
HEPES, pH 7.9, MgCl2, KCL, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors.
Concentration
5.0 mg/ml

Lysate Details for Bladder

Type
Tissue
Life Stage
Adult
Tissue
Digestive/Waste
Protein State
Native
Tissue Condition
Normal
Subcellular Fraction
Whole

Notes

We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.

Background

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Bladder Lysate (NB820-59173) (0)

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Positive Control Lysate(s)

Additional Bladder Products

Bladder NB820-59173

Blogs on Bladder

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