AGER Antibody (176902) [Unconjugated]

Western blot shows lysates of human lung tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human RAGE Monoclonal Antibody (Catalog # MAB11451) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for RAGE at approximately 43 kDa under non-reducing (NR) conditions and 50 kDa under reducing (R) conditions (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, B/N, ELISA(Cap)
• Clone: 176902
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

AGER Antibody (176902) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human RAGE
  Gln24-Ala344
  Accession # Q15109
• Specificity: Detects human RAGE in ELISAs and Western blots. In Western blots, approximately 25% cross-reactivity with recombinant mouse RAGE and recombinant rat RAGE is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: AGER
• Endotoxin Note: <0.01 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL
  • Blockade of Receptor-ligand Interaction
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Detection: Human RAGE Biotinylated Antibody (Catalog number BAF1145)
  Standard: Recombinant Human RAGE Fc Chimera (Catalog number 1145-RG)
• Control:

  RAGE Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for AGER Antibody (176902) [Unconjugated]

• advanced glycosylation end product-specific receptor
• AGER
• RAGE isoform delta
• RAGE isoform sRAGE-delta
• RAGE
• Receptor for advanced glycosylation end products
• receptor for advanced glycosylation end-products
• SCARJ1

Background

Advanced glycation endproducts (AGE) are adducts formed by the non-enzymatic glycation or oxidation of macromolecules (1). AGE forms during aging and its formation is accelerated under pathophysiologic states such as diabetes, Alzheimer’s disease, renal failure and immune/inflammatory disorders. Receptor for Advanced Glycation Endoproducts (RAGE), named for its ability to bind AGE, is a multi-ligand receptor belonging the immunoglobulin (Ig) superfamily. Besides AGE, RAGE binds amyloid beta -peptide, S100/calgranulin family proteins, high mobility group B1 (HMGB1, also know as amphoterin) and leukocyte integrins (1, 2).

The human RAGE gene encodes a 404 amino acid residues (aa) type I transmembrane glycoprotein with a 22 aa signal peptide, a 320 aa extracellular domain containing an Ig-like V-type domain and two Ig-like Ce-type domains, a 21 aa transmembrane domain and a 41 aa cytoplasmic domain (3). The V-type domain and the cytoplasmic domain are important for ligand binding and for intracellular signaling, respectively. Two alternative splice variants, lacking the V-type domain or the cytoplasmic tail, are known (1, 4). RAGE is highly expressed in the embryonic central nervous system (5). In adult tissues, RAGE is expressed at low levels in multiple tissues including endothelial and smooth muscle cells, mononuclear phagocytes, pericytes, microglia, neurons, cardiac myocytes and hepatocytes (6). The expression of RAGE is upregulated upon ligand interaction. Depending on the cellular context and interacting ligand, RAGE activation can trigger differential signaling pathways that affect divergent pathways of gene expression (1, 7). RAGE activation modulates varied essential cellular responses (including inflammation, immunity, proliferation, cellular adhesion and migration) that contribute to cellular dysfunction associated with chronic diseases such as diabetes, cancer, amyloidoses and immune or inflammatory disorders (1).

1. Schmidt, A. et al. (2001) J. Clin. Invest. 108:949.
2. Chavakis, T. et al. (2003) J. Exp. Med. 198:507.
3. Neeper, M. et al. (1992) J. Biol. Chem. 267:14998.
4. Yonekura, H. et al. (2003) Biochem. J. 370:1097.
5. Hori, O. et al. (1995) J. Biol. Chem. 270:25752.
6. Brett, J. et al. (1993) Am. J. Pathol. 143:1699.
7. Valencia, J.V. et al. (2004) Diabetes 53:743.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AGER Antibody (MAB11451)(10)

Publications using MAB11451

• J Wu, Z Deng, M Sun, W Zhang, Y Yang, Z Zeng, J Wu, Q Zhang, Y Liu, Z Chen, X Guo, KS Zhao, Q Huang, Z Chen Polydatin protects against lipopolysaccharide-induced endothelial barrier disruption via SIRT3 activation Lab. Invest., 2019;0(0):. 2019 [PMID: 31641228] (Neutralization, Human)
• RJ Pickering, C Tikellis, CJ Rosado, D Tsorotes, A Dimitropou, M Smith, O Huet, RM Seeber, R Abhayaward, EK Johnstone, J Golledge, Y Wang, KA Jandeleit-, ME Cooper, KD Pfleger, MC Thomas Transactivation of RAGE mediates angiotensin-induced inflammation and atherogenesis J. Clin. Invest., 2019;129(1):406-421. 2019 [PMID: 30530993] (Bioassay, Mouse)
• B Zhou, P Flodby, J Luo, DR Castillo, Y Liu, FX Yu, A McConnell, B Varghese, G Li, NO Chimge, M Sunohara, MN Koss, W Elatre, P Conti, JM Liebler, C Yang, CN Marconett, IA Laird-Offr, P Minoo, K Guan, BR Stripp, ED Crandall, Z Borok Claudin-18-mediated YAP activity regulates lung stem and progenitor cell homeostasis and tumorigenesis J. Clin. Invest., 2018;0(0):. 2018 [PMID: 29400695] (Western Blot, Mouse)
• J Robert, EB Button, S Stukas, GK Boyce, E Gibbs, CM Cowan, M Gilmour, WH Cheng, SK Soo, B Yuen, A Bahrabadi, K Kang, I Kulic, G Francis, N Cashman, CL Wellington High-density lipoproteins suppress A?-induced PBMC adhesion to human endothelial cells in bioengineered vessels and in monoculture Mol Neurodegener, 2017;12(1):60. 2017 [PMID: 28830501] (Neutralization, Human)
• Liu W, Shen T, Chen R, Wu H, Wang Y, Deng J, Chen Q, Pan Q, Huang Fu C, Tao J, Liang D, Liu H Autophagy-Lysosome Pathway in Renal Tubular Epithelial Cells Is Disrupted by Advanced Glycation End Products in Diabetic Nephropathy. J Biol Chem, 2015;290(33):20499-510. 2015 [PMID: 26100632] (Neutralization, Human)
• Tuinman P, Cornet A, Kuipers M, Vlaar A, Schultz M, Beishuizen A, Groeneveld A, Juffermans N Soluble receptor for advanced glycation end products as an indicator of pulmonary vascular injury after cardiac surgery. BMC Pulm Med, 2013;13(0):76. 2013 [PMID: 24341821] (ELISA Development, Human)
• Yamakawa N, Uchida T, Matthay MA, Makita K Proteolytic release of the receptor for advanced glycation end products from in vitro and in situ alveolar epithelial cells. Am. J. Physiol. Lung Cell Mol. Physiol., 2011;300(4):L516-25. 2011 [PMID: 21257730] (ELISA Development, Rat)
• Tam HL, Shiu SW, Wong Y, Chow WS, Betteridge DJ, Tan KC Effects of atorvastatin on serum soluble receptors for advanced glycation end-products in type 2 diabetes. Atherosclerosis, 2010;209(1):173-7. 2010 [PMID: 19733353] (Western Blot, Human)
• Sakaguchi M, Sonegawa H, Murata H, Kitazoe M, Futami J, Kataoka K, Yamada H, Huh NH S100A11, an dual mediator for growth regulation of human keratinocytes. Mol. Biol. Cell, 2007;19(1):78-85. 2007 [PMID: 17978094] (Western Blot, Human)
• Zen K, Chen CX, Chen YT, Wilton R, Liu Y Receptor for advanced glycation endproducts mediates neutrophil migration across intestinal epithelium. J. Immunol., 2007;178(4):2483-90. 2007 [PMID: 17277156] (Western Blot, Human)

Bioinformatics

• Gene Symbol: AGER
• Uniprot:
  • Q15109()