AGER Antibody (176902) [Unconjugated]

Western blot shows lysates of human lung tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human RAGE Monoclonal Antibody (Catalog # MAB11451) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for RAGE at approximately 43 kDa under non-reducing (NR) conditions and 50 kDa under reducing (R) conditions (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

The effects of blocking TLR4 and RAGE on S100A8, S100A9 and S100A8/A9 stimulation of HUVECs.HUVECs were stimulated with S100A8 (2.0 µg/mL) (A, D), S100A9 (2.0 µg/mL) (B, E) and S100A8/A9 (2.0 µg/mL) (C, F) for 120 min with or without 60 min pre-incubation with specific blockers (TAK242 (5 µM) for TLR4 and anti-human RAGE antibody (10 µg/mL) for RAGE). Then the TER was measured (A, B and C). All data are presented as mean ± s.d. of four independent experiments. Phosphorylation of p38 (p-p38) and ERK1/2 (p-ERK1/2) were also assessed by Western blotting (D, E and F). The ratio of immunointensity between the phosphorylation of p38 and ERK1/2 (p-p38 and p-ERK) and total p38 and ERK1/2 (p38 and ERK) were calculated (D, E and F). The results are expressed in mean ± s.d. from three independent experiments. **P<0.01 vs. Control; #P<0.05 vs. S100A8/S100A9; ♣P<0.05 vs. S100A8/S100A9+TAK242+anti-RAGE. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/24595267), licensed under a CC-BY license. Not internally tested by R&D Systems.

The effects of blocking TLR4 and RAGE on S100A8, S100A9 and S100A8/A9 stimulation of HUVECs.HUVECs were stimulated with S100A8 (2.0 µg/mL) (A, D), S100A9 (2.0 µg/mL) (B, E) and S100A8/A9 (2.0 µg/mL) (C, F) for 120 min with or without 60 min pre-incubation with specific blockers (TAK242 (5 µM) for TLR4 and anti-human RAGE antibody (10 µg/mL) for RAGE). Then the TER was measured (A, B and C). All data are presented as mean ± s.d. of four independent experiments. Phosphorylation of p38 (p-p38) and ERK1/2 (p-ERK1/2) were also assessed by Western blotting (D, E and F). The ratio of immunointensity between the phosphorylation of p38 and ERK1/2 (p-p38 and p-ERK) and total p38 and ERK1/2 (p38 and ERK) were calculated (D, E and F). The results are expressed in mean ± s.d. from three independent experiments. **P<0.01 vs. Control; #P<0.05 vs. S100A8/S100A9; ♣P<0.05 vs. S100A8/S100A9+TAK242+anti-RAGE. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/24595267), licensed under a CC-BY license. Not internally tested by R&D Systems.

The effects of blocking TLR4 and RAGE on S100A8, S100A9 and S100A8/A9 stimulation of HUVECs.HUVECs were stimulated with S100A8 (2.0 µg/mL) (A, D), S100A9 (2.0 µg/mL) (B, E) and S100A8/A9 (2.0 µg/mL) (C, F) for 120 min with or without 60 min pre-incubation with specific blockers (TAK242 (5 µM) for TLR4 and anti-human RAGE antibody (10 µg/mL) for RAGE). Then the TER was measured (A, B and C). All data are presented as mean ± s.d. of four independent experiments. Phosphorylation of p38 (p-p38) and ERK1/2 (p-ERK1/2) were also assessed by Western blotting (D, E and F). The ratio of immunointensity between the phosphorylation of p38 and ERK1/2 (p-p38 and p-ERK) and total p38 and ERK1/2 (p38 and ERK) were calculated (D, E and F). The results are expressed in mean ± s.d. from three independent experiments. **P<0.01 vs. Control; #P<0.05 vs. S100A8/S100A9; ♣P<0.05 vs. S100A8/S100A9+TAK242+anti-RAGE. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/24595267), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, B/N, ELISA(Cap)
• Clone: 176902
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

AGER Antibody (176902) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human RAGE
  Gln24-Ala344
  Accession # Q15109
• Specificity: Detects human RAGE in ELISAs and Western blots. In Western blots, approximately 25% cross-reactivity with recombinant mouse RAGE and recombinant rat RAGE is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: AGER
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 2 ug/mL
• Application Notes: ELISA Detection: Human RAGE Biotinylated Antibody (Catalog number BAF1145)
  Standard: Recombinant Human RAGE Fc Chimera (Catalog number 1145-RG)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for AGER Antibody (176902) [Unconjugated]

• advanced glycosylation end product-specific receptor
• AGER
• RAGE isoform delta
• RAGE isoform sRAGE-delta
• RAGE
• Receptor for advanced glycosylation end products
• receptor for advanced glycosylation end-products
• SCARJ1

Background

Advanced glycation endproducts (AGE) are adducts formed by the non-enzymatic glycation or oxidation of macromolecules (1). AGE forms during aging and its formation is accelerated under pathophysiologic states such as diabetes, Alzheimer’s disease, renal failure and immune/inflammatory disorders. Receptor for Advanced Glycation Endoproducts (RAGE), named for its ability to bind AGE, is a multi-ligand receptor belonging the immunoglobulin (Ig) superfamily. Besides AGE, RAGE binds amyloid beta -peptide, S100/calgranulin family proteins, high mobility group B1 (HMGB1, also know as amphoterin) and leukocyte integrins (1, 2).

The human RAGE gene encodes a 404 amino acid residues (aa) type I transmembrane glycoprotein with a 22 aa signal peptide, a 320 aa extracellular domain containing an Ig-like V-type domain and two Ig-like Ce-type domains, a 21 aa transmembrane domain and a 41 aa cytoplasmic domain (3). The V-type domain and the cytoplasmic domain are important for ligand binding and for intracellular signaling, respectively. Two alternative splice variants, lacking the V-type domain or the cytoplasmic tail, are known (1, 4). RAGE is highly expressed in the embryonic central nervous system (5). In adult tissues, RAGE is expressed at low levels in multiple tissues including endothelial and smooth muscle cells, mononuclear phagocytes, pericytes, microglia, neurons, cardiac myocytes and hepatocytes (6). The expression of RAGE is upregulated upon ligand interaction. Depending on the cellular context and interacting ligand, RAGE activation can trigger differential signaling pathways that affect divergent pathways of gene expression (1, 7). RAGE activation modulates varied essential cellular responses (including inflammation, immunity, proliferation, cellular adhesion and migration) that contribute to cellular dysfunction associated with chronic diseases such as diabetes, cancer, amyloidoses and immune or inflammatory disorders (1).

1. Schmidt, A. et al. (2001) J. Clin. Invest. 108:949.
2. Chavakis, T. et al. (2003) J. Exp. Med. 198:507.
3. Neeper, M. et al. (1992) J. Biol. Chem. 267:14998.
4. Yonekura, H. et al. (2003) Biochem. J. 370:1097.
5. Hori, O. et al. (1995) J. Biol. Chem. 270:25752.
6. Brett, J. et al. (1993) Am. J. Pathol. 143:1699.
7. Valencia, J.V. et al. (2004) Diabetes 53:743.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AGER Antibody (MAB11451)(34)

Publications using MAB11451

• Nguyen, DH;Tian, J;Shanahan, SL;Wang, CK;Jacks, T;Wang, X;Li, P; A tissue-scale strategy for sensing threats in barrier organs bioRxiv : the preprint server for biology 2025-03-19 [PMID: 40166266] (Immunohistochemistry, Mouse)
• Schwertner, K;Gelles, K;Leitner, J;Steinberger, P;Gundacker, C;Vrticka, R;Hoffmann-Sommergruber, K;Ellinger, I;Geiselhart, S; Human intestine and placenta exhibit tissue-specific expression of RAGE isoforms Heliyon 2023-08-01 [PMID: 37533998] (Flow Cytometry, ICC, Western Blot, Human)
• Han Z, Andr� M, Madhavan BK et al. The importance of nuclear RAGE-Mcm2 axis in diabetes or cancer-associated replication stress Nucleic acids research 2023-03-21 [PMID: 36807739]
• Han Z, Andr� M, Madhavan BK et al. The importance of nuclear RAGE-Mcm2 axis in diabetes or cancer-associated replication stress Nucleic acids research 3/21/2023 [PMID: 36807739]
• Valerie R. Wiersma, Susan J. M. Hoonhorst, Nick H. T. ten Hacken, Maarten van den Berge, Dirk-Jan Slebos, Simon D. Pouwels The Decrease in Serum sRAGE Levels Upon Smoking is Associated with Activated Neutrophils Lung 12/1/2022 [PMID: 36282357]
• T Kadonosono, K Miyamoto, S Sakai, Y Matsuo, S Kitajima, Q Wang, M Endo, M Niibori, T Kuchimaru, T Soga, K Hirota, S Kizaka-Kon AGE/RAGE axis regulates reversible transition to quiescent states of ALK-rearranged NSCLC and pancreatic cancer cells in monolayer cultures Oncogene, 2022-06-14;12(1):9886. 2022-06-14 [PMID: 35701529] (Neutralization, Human)
• F Klont, P Horvatovic, RP Bowler, E van Rikxoo, JP Charbonnie, M Kwiatkowsk, DA Lynch, S Humphries, R Bischoff, NHT Ten Hacken, SD Pouwels Plasma sRAGE levels strongly associate with centrilobular emphysema assessed by HRCT scans Respiratory Research, 2022-01-24;23(1):15. 2022-01-24 [PMID: 35073932] (Immunoprecipitation, Human)
• Helena Bui, Amena Keshawarz, Shih-Jen Hwang, Chen Yao, Gha Young Lee, Kathryn Recto et al. A genomic approach identifies sRAGE as a putatively causal protein for asthma Journal of Allergy and Clinical Immunology 6/1/2022 [PMID: 34974068]
• Hannah E. Zenker, Malgorzata Teodorowicz, Harry J. Wichers, Kasper A. Hettinga No Glycation Required: Interference of Casein in AGE Receptor Binding Tests Foods 8/9/2021 [PMID: 34441613]
• Xiang Zhong, Fengwen Xie, Li Chen, Zhixing Liu, Qun Wang S100A8 and S100A9 promote endothelial cell activation through the RAGE‑mediated mammalian target of rapamycin complex 2 pathway Molecular Medicine Reports 10/14/2020 [PMID: 33174028]
• Y Deng, C Govers, M Teodorowic, I Liobyte, I De Simone, K Hettinga, HJ Wichers Hydrophobicity drives receptor-mediated uptake of heat-processed proteins by THP-1 macrophages and dendritic cells, but not cytokine responses PLoS ONE, 2020-08-14;15(8):e0236212. 2020-08-14 [PMID: 32797100] (ELISA Detection, Human)
• V Rolfes, LS Ribeiro, I Hawwari, L Böttcher, N Rosero, S Maasewerd, MLS Santos, T Próchnicki, CMS Silva, CWS Wanderley, M Rothe, SV Schmidt, HJ Stunden, D Bertheloot, MN Rivas, CJ Fontes, LH Carvalho, FQ Cunha, E Latz, M Arditi, BS Franklin Platelets Fuel the Inflammasome Activation of Innate Immune Cells Cell Rep, 2020-05-12;31(6):107615. 2020-05-12 [PMID: 32402278] (Inhibition, Human)
• J Robert, EB Button, EM Martin, L McAlary, Z Gidden, M Gilmour, G Boyce, TM Caffrey, A Agbay, A Clark, JM Silverman, NR Cashman, CL Wellington Cerebrovascular amyloid Angiopathy in bioengineered vessels is reduced by high-density lipoprotein particles enriched in Apolipoprotein E Mol Neurodegener, 2020-03-25;15(1):23. 2020-03-25 [PMID: 32213187] (Neutralization, Human)
• Gareth D. Healey, Belen Pan-Castillo, Jezabel Garcia-Parra, Julia Davies, Shaun Roberts, Eilir Jones et al. Antibody drug conjugates against the receptor for advanced glycation end products (RAGE), a novel therapeutic target in endometrial cancer Journal for ImmunoTherapy of Cancer 12/1/2019 [PMID: 31665084]
• J Wu, Z Deng, M Sun, W Zhang, Y Yang, Z Zeng, J Wu, Q Zhang, Y Liu, Z Chen, X Guo, KS Zhao, Q Huang, Z Chen Polydatin protects against lipopolysaccharide-induced endothelial barrier disruption via SIRT3 activation Lab. Invest., 2019-10-22;0(0):. 2019-10-22 [PMID: 31641228] (Neutralization, Human)
• Hannah E. Zenker, Arifa Ewaz, Ying Deng, Huub F. J. Savelkoul, R.J. Joost van Neerven, Nicolette W. De Jong et al. Differential Effects of Dry vs. Wet Heating of beta -Lactoglobulin on Formation of sRAGE Binding Ligands and sIgE Epitope Recognition Nutrients 6/25/2019 [PMID: 31242665]
• RJ Pickering, C Tikellis, CJ Rosado, D Tsorotes, A Dimitropou, M Smith, O Huet, RM Seeber, R Abhayaward, EK Johnstone, J Golledge, Y Wang, KA Jandeleit-, ME Cooper, KD Pfleger, MC Thomas Transactivation of RAGE mediates angiotensin-induced inflammation and atherogenesis J. Clin. Invest., 2018-12-10;129(1):406-421. 2018-12-10 [PMID: 30530993] (Bioassay, Mouse)
• B Zhou, P Flodby, J Luo, DR Castillo, Y Liu, FX Yu, A McConnell, B Varghese, G Li, NO Chimge, M Sunohara, MN Koss, W Elatre, P Conti, JM Liebler, C Yang, CN Marconett, IA Laird-Offr, P Minoo, K Guan, BR Stripp, ED Crandall, Z Borok Claudin-18-mediated YAP activity regulates lung stem and progenitor cell homeostasis and tumorigenesis J. Clin. Invest., 2018-02-05;0(0):. 2018-02-05 [PMID: 29400695] (Western Blot, Mouse)
• J Robert, EB Button, S Stukas, GK Boyce, E Gibbs, CM Cowan, M Gilmour, WH Cheng, SK Soo, B Yuen, A Bahrabadi, K Kang, I Kulic, G Francis, N Cashman, CL Wellington High-density lipoproteins suppress A?-induced PBMC adhesion to human endothelial cells in bioengineered vessels and in monoculture Mol Neurodegener, 2017-08-22;12(1):60. 2017-08-22 [PMID: 28830501] (Neutralization, Human)
• Chakraborty Paramita, Bjork Per, Kallberg Eva et al. Vesicular Location and Transport of S100A8 and S100A9 Proteins in Monocytoid Cells. PloS One 1/1/2015 [PMID: 26661255]
• Fahui Liu, Małgorzata Teodorowicz, Martinus A. J. S. van Boekel, Harry J. Wichers, Kasper A. Hettinga The decrease in the IgG-binding capacity of intensively dry heated whey proteins is associated with intense Maillard reaction, structural changes of the proteins and formation of RAGE-ligands Food & Function 1/1/2016 [PMID: 26524422]
• Siao-Syun Guan, Meei-Ling Sheu, Cheng-Tien Wu, Chih-Kang Chiang, Shing-Hwa Liu ATP synthase subunit-beta down-regulation aggravates diabetic nephropathy Scientific Reports 2015-10-09 [PMID: 26449648] (Bioassay, Human)
• Siao-Syun Guan, Meei-Ling Sheu, Cheng-Tien Wu, Chih-Kang Chiang, Shing-Hwa Liu ATP synthase subunit-beta down-regulation aggravates diabetic nephropathy Scientific Reports 10/9/2015 [PMID: 26449648]
• Sung Wook Park, Jin Hyoung Kim, Sang Min Park, Minho Moon, Ki Hwang Lee, Kyu Hyung Park et al. RAGE mediated intracellular A beta uptake contributes to the breakdown of tight junction in retinal pigment epithelium Oncotarget 11/3/2015 [PMID: 26431165]
• Xinyu Yang, Haichao Wang, Menmen Zhang, Jin Liu, Ben Lv, Fangping Chen RETRACTED ARTICLE: HMGB1: a novel protein that induced platelets active and aggregation via Toll-like receptor-4, NF-kappa B and cGMP dependent mechanisms Diagnostic Pathology 12/1/2015 [PMID: 26245198]
• Liu W, Shen T, Chen R, Wu H, Wang Y, Deng J, Chen Q, Pan Q, Huang Fu C, Tao J, Liang D, Liu H Autophagy-Lysosome Pathway in Renal Tubular Epithelial Cells Is Disrupted by Advanced Glycation End Products in Diabetic Nephropathy. J Biol Chem, 2015-06-22;290(33):20499-510. 2015-06-22 [PMID: 26100632] (Neutralization, Human)
• Wang Y, Fang C, Gao H et al. Platelet-derived S100 family member myeloid-related protein-14 regulates thrombosis. J. Clin. Invest. 4/1/2014 [PMID: 24691441]
• Liqun Wang, Haihua Luo, Xiaohuan Chen, Yong Jiang, Qiaobing Huang Functional Characterization of S100A8 and S100A9 in Altering Monolayer Permeability of Human Umbilical Endothelial Cells PLoS ONE 3/3/2014 [PMID: 24595267]
• Tuinman P, Cornet A, Kuipers M, Vlaar A, Schultz M, Beishuizen A, Groeneveld A, Juffermans N Soluble receptor for advanced glycation end products as an indicator of pulmonary vascular injury after cardiac surgery. BMC Pulm Med, 2013-12-16;13(0):76. 2013-12-16 [PMID: 24341821] (ELISA Development, Human)
• Yamakawa N, Uchida T, Matthay MA, Makita K Proteolytic release of the receptor for advanced glycation end products from in vitro and in situ alveolar epithelial cells. Am. J. Physiol. Lung Cell Mol. Physiol., 2011-01-21;300(4):L516-25. 2011-01-21 [PMID: 21257730] (ELISA Development, Rat)
• Tam HL, Shiu SW, Wong Y, Chow WS, Betteridge DJ, Tan KC Effects of atorvastatin on serum soluble receptors for advanced glycation end-products in type 2 diabetes. Atherosclerosis, 2009-08-21;209(1):173-7. 2009-08-21 [PMID: 19733353] (Western Blot, Human)
• Haichao Wang, Haichao Tang, Yiting Tang, Zhang Fan, Xianzhong Xiao, Xianzhong Chen High-mobility group box 1 protein induces tissue factor expression in vascular endothelial cells via activation of NF-kappaB and Egr-1 Thrombosis and Haemostasis 4/16/2010 [PMID: 19652887]
• Sakaguchi M, Sonegawa H, Murata H, Kitazoe M, Futami J, Kataoka K, Yamada H, Huh NH S100A11, an dual mediator for growth regulation of human keratinocytes. Mol. Biol. Cell, 2007-10-31;19(1):78-85. 2007-10-31 [PMID: 17978094] (Neutralization, Western Blot, Human)
• Zen K, Chen CX, Chen YT, Wilton R, Liu Y Receptor for advanced glycation endproducts mediates neutrophil migration across intestinal epithelium. J. Immunol., 2007-02-15;178(4):2483-90. 2007-02-15 [PMID: 17277156] (IHC-Fr, Western Blot, Human)

Bioinformatics

• Gene Symbol: AGER
• Uniprot:
  • Q15109()