AGER Antibody (175410) [Unconjugated]

Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse/Rat RAGE Monoclonal Antibody (Catalog # 1179) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for RAGE and mature RAGE at approximately 50 and 38 kDa, respectively (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

RAGE was detected in perfusion fixed frozen sections of adult mouse lung using Rat Anti-Mouse/Rat RAGE Monoclonal Antibody (Catalog # MAB1179) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Northern­Lights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu, Rt
• Applications: WB, IHC
• Clone: 175410
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

AGER Antibody (175410) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse RAGE
  Gly23-Ala342
  Accession # NP_031451
• Specificity: Detects mouse and rat RAGE in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human RAGE is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: Ager

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for AGER Antibody (175410) [Unconjugated]

• advanced glycosylation end product-specific receptor
• AGER
• RAGE isoform delta
• RAGE isoform sRAGE-delta
• RAGE
• Receptor for advanced glycosylation end products
• receptor for advanced glycosylation end-products
• SCARJ1

Background

Advanced glycation endproducts (AGE) are adducts formed by the non-enzymatic glycation or oxidation of macromolecules (1). AGE forms during aging and its formation is accelerated under pathophysiologic states such as diabetes, Alzheimer’s disease, renal failure and immune/inflammatory disorders. Receptor for Advanced Glycation Endoproducts (RAGE), named for its ability to bind AGE, is a multiligand receptor belonging the immunoglobulin (Ig) superfamily. Besides AGE, RAGE binds amyloid beta -peptide, S100/calgranulin family proteins, high mobility group B1 (HMGB1, also know as amphoterin) and leukocyte integrins (1, 2). The mouse RAGE gene encodes a 403 amino acid (aa) residue type I transmembrane glycoprotein with a 22 aa signal peptide, a 319 aa extracellular domain containing a Ig-like V‑type domain and two Ig-like Ce-type domains, a 21 aa transmembrane domain and a 41 aa cytoplasmic domain (3). The V‑type domain and the cytoplasmic domain are important for ligand binding and for intracellular signaling, respectively. Two alternative splice variants, lacking the V‑type domain or the cytoplasmic tail, are known (1, 4). RAGE is highly expressed in the embryonic central nervous system (5). In adult tissues, RAGE is expressed at low levels in multiple tissues including endothelial and smooth muscle cells, mononuclear phagocytes, pericytes, microglia, neurons, cardiac myocytes and hepatocytes (6). The expression of RAGE is upregulated upon ligand interaction. Depending on the cellular context and interacting ligand, RAGE activation can trigger differential signaling pathways that affect divergent pathways of gene expression (1, 7). RAGE activation modulates varied essential cellular responses (including inflammation, immunity, proliferation, cellular adhesion and migration) that contribute to cellular dysfunction associated with chronic diseases such as diabetes, cancer, amyloidoses, and immune or inflammatory disorders (1).

1. Schmidt, A. et al. (2001) J. Clin. Invest. 108:949.
2. Chavakis, T. et al. (2003) J. Exp. Med. 198:507.
3. Renard, C. et al. (1997) Mol. Pharmacol. 52:54.
4. Yonekura, H. et al. (2003) Biochem. J. 370:1097.
5. Hori, O. et al. (1995) J. Biol. Chem. 270:25752.
6. Brett, J. et al. (1993) Am. J. Pathol. 143:1699.
7. Valencia, J.V. et al. (2004) Diabetes 53:743.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AGER Antibody (MAB1179)(12)

Publications using MAB1179

• H Katsura, Y Kobayashi, PR Tata, BLM Hogan IL-1 and TNF? Contribute to the Inflammatory Niche to Enhance Alveolar Regeneration Stem Cell Reports, 2019;0(0):. 2019 [PMID: 30930244] (IHC-P, Mouse)
• K Kato, R Diéguez-Hu, DY Park, SP Hong, S Kato-Azuma, S Adams, M Stehling, B Trappmann, JL Wrana, GY Koh, RH Adams Pulmonary pericytes regulate lung morphogenesis Nat Commun, 2018;9(1):2448. 2018 [PMID: 29934496] (IHC-P, Mouse)
• HY Kim, X Wang, R Kang, D Tang, BA Boone, HJ Zeh, MT Lotze, WB Edwards RAGE-specific single chain Fv for PET imaging of pancreatic cancer PLoS ONE, 2018;13(3):e0192821. 2018 [PMID: 29529089] (Flow Cytometry, Mouse)
• H Matsumoto, N Matsumoto, J Shimazaki, J Nakagawa, Y Imamura, K Yamakawa, T Yamada, M Ikeda, H Hiraike, H Ogura, T Shimazu Therapeutic Effectiveness of Anti-RAGE Antibody Administration in a Rat Model of Crush Injury Sci Rep, 2017;7(1):12255. 2017 [PMID: 28947744] (IHC, Rat)
• AJ Lechner, IH Driver, J Lee, CM Conroy, A Nagle, RM Locksley, JR Rock Recruited Monocytes and Type 2 Immunity Promote Lung Regeneration following Pneumonectomy Cell Stem Cell, 2017;0(0):. 2017 [PMID: 28506464] (IHC, Mouse)
• J Yang, BJ Hernandez, D Martinez A, O Narvaez de, L Vila-Ellis, H Akiyama, SE Evans, EJ Ostrin, J Chen The development and plasticity of alveolar type 1 cells Development, 2016;143(1):54-65. 2016 [PMID: 26586225] (ICC, Mouse)
• Alanis, Denise M, Chang, Daniel R, Akiyama, Haruhiko, Krasnow, Mark A, Chen, Jichao Two nested developmental waves demarcate a compartment boundary in the mouse lung. Nat Commun, 2014;5(0):3923. 2014 [PMID: 24879355] (IHC, Mouse)
• Desai, Tushar J, Brownfield, Douglas, Krasnow, Mark A Alveolar progenitor and stem cells in lung development, renewal and cancer. Nature, 2014;507(7491):190-4. 2014 [PMID: 24499815] (IHC-Fr, Mouse)
• Mizumoto S, Takahashi J, Sugahara K Receptor for advanced glycation end products (RAGE) functions as receptor for specific sulfated glycosaminoglycans, and anti-RAGE antibody or sulfated glycosaminoglycans delivered in vivo inhibit pulmonary metastasis of tumor cells. J. Biol. Chem., 2012;287(23):18985-94. 2012 [PMID: 22493510] (Neutralization, Mouse)
• Hallam KM, Li Q, Ananthakrishnan R, Kalea A, Zou YS, Vedantham S, Schmidt AM, Yan SF, Ramasamy R Aldose reductase and AGE-RAGE pathways: central roles in the pathogenesis of vascular dysfunction in aging rats. Aging Cell, 2010;9(5):776-84. 2010 [PMID: 20670350] (Western Blot, Rat)
• Zhang H, Tasaka S, Shiraishi Y, Fukunaga K, Yamada W, Seki H, Ogawa Y, Miyamoto K, Nakano Y, Hasegawa N, Miyasho T, Maruyama I, Ishizaka A Role of soluble receptor for advanced glycation end products on endotoxin-induced lung injury. Am. J. Respir. Crit. Care Med., 2008;178(4):356-62. 2008 [PMID: 18535257] (Western Blot, Mouse)
• Parmley LA, Elkins ND, Fini MA, Liu YE, Repine JE, Wright RM Alpha-4/beta-1 and alpha-L/beta-2 integrins mediate cytokine induced lung leukocyte-epithelial adhesion and injury. Br. J. Pharmacol., 2007;152(6):915-29. 2007 [PMID: 17828290] (Western Blot, Rat)

Bioinformatics

• Gene Symbol: Ager
• Uniprot:
  • NP_031451()