AG-2 Antibody

Images

 
Western Blot: AG-2 Antibody [NBP1-05936] - Human stomach lysate.
Immunocytochemistry/ Immunofluorescence: AG-2 Antibody [NBP1-05936] - AGR2 antibody was tested in MCF7 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry: AG-2 Antibody [NBP1-05936] - Detection of AGR2 in mouse kidney.
Immunohistochemistry: AG-2 Antibody [NBP1-05936] - Detection of AGR2 in mouse prostate.

Product Details

Summary
Reactivity Hu, Mu, BvSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

AG-2 Antibody Summary

Immunogen
Synthetic peptide made to an N-terminal portion of mouse AGR2 (within residues 1-50). [Swiss-Prot# O88312]
Localization
Secreted
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AGR2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1.0 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:100-1:300
  • Immunohistochemistry-Paraffin 1:100-1:300
Application Notes
This AGR2 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections, and Western blot analysis where a band can be seen at ~19.9 kDa. In ICC/IF cytoplasmic staining is observed. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
AG-2 Peptide (NBP1-05936PEP)

Reactivity Notes

Human, mouse and bovine.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for AG-2 Antibody

  • AG2
  • AG-2
  • AG2member 17
  • AGR2
  • anterior gradient 2 homolog (Xenopus laevis)
  • anterior gradient homolog 2 (Xenopus laevis)
  • GOB-4
  • hAG-2
  • HPC8
  • PDIA17
  • secreted cement gland homolog

Background

Originally discovered in Xenopus laevis wherein it plays a role during early neural development, AGR2 (anterior gradient protein 2 homolog) is now known as a member of protein disulfide isomerase (PDI) family of ER-resident proteins which is expressed in a variety of normal as well as transformed epithelial tissues. AGR2 has been implicated in the folding of proteins as a chaperone protein. It interacts with LYPD3, DAG1 (alphaDAG1) and MUC2 (disulfide-linked), and is essential for MUC2 translation as well as secretion. AGR2 plays a key role in the production of mucus by intestinal cells and has also been suggested to be a proto-oncogene that may involve in cellular migration, differentiation and growth. Differential expression of AGR2 was first found in estrogen receptor (ER)-positive breast cancer cell lines and subsequently, elevated AGR2 levels have been seen in subsets of other cancers also wherein its upregulation enhances classical hallmarks of cancer such as metastasis, invasion, colony formation, and proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for AG-2 Antibody (NBP1-05936) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-05936AF405
Alexa Fluor 488 Labeled NBP1-05936AF488
Alexa Fluor 647 Labeled NBP1-05936AF647
Alexa Fluor 700 Labeled NBP1-05936AF700
Biotin Labeled NBP1-05936B
DyLight 405 Labeled NBP1-05936V
DyLight 488 Labeled NBP1-05936G
DyLight 550 Labeled NBP1-05936R
DyLight 650 Labeled NBP1-05936C
FITC Labeled NBP1-05936F
HRP Labeled NBP1-05936H

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Bioinformatics

Gene Symbol AGR2
Entrez