AFMID Overexpression Lysate (Native)

Images

 
Western Blot: AFMID Overexpression Lysate (Adult Normal) [NBP2-08777] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for AFMID.

Product Details

Summary
Applications WB

Order Details

AFMID Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_001010982. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens AFMID, mRNA.
Gene
AFMID

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
33.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Lysate Details for AFMID

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for AFMID Overexpression Lysate (Native)

  • arylformamidase
  • kynurenine formamidase
  • MGC167063

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

H00009771-B01P
Species: Hu
Applications: WB, ELISA, ICC/IF
NBP2-02002
Species: Hu
Applications: WB, Flow, IHC, IHC-P
NB600-41532
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP1-30027
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-24916
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P
NBP1-83299
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP1-86616
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
H00003347-M01
Species: Hu
Applications: ELISA, ICC/IF
NBP2-45897
Species: Hu
Applications: WB, IHC
NBP1-91996
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
NBP1-91012
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-90306
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-59069
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NB600-1025
Species: Hu, Mu, Rt, Bv, Ha, Xp
Applications: WB, IHC, IHC-P, IP
NBP1-88116
Species: Hu
Applications: ICC/IF, IHC, IHC-P
MAB5197
Species: Hu
Applications: Flow, CyTOF-ready
NBP2-08777
Applications: WB

Publications for AFMID Lysate (NBP2-08777) (0)

There are no publications for AFMID Lysate (NBP2-08777).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for AFMID Lysate (NBP2-08777) (0)

There are no reviews for AFMID Lysate (NBP2-08777). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for AFMID Lysate (NBP2-08777) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional AFMID Overexpression Lysate (Native) Products

Related Products by Gene

Bioinformatics Tool for AFMID Lysate (NBP2-08777)

Discover related pathways, diseases and genes to AFMID Lysate (NBP2-08777). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for AFMID Lysate (NBP2-08777)

Discover more about diseases related to AFMID Lysate (NBP2-08777).

Blogs on AFMID

There are no specific blogs for AFMID, but you can read our latest blog posts.

Contact Information

Product PDFs

Review this Product

Be the first to review our AFMID Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol AFMID

Customers Who Bought This Also Bought

AFMID Antibody
NBP1-94068
AFMID Antibody
NBP1-94068
Albumin Antibody
NB600-41532