AF9 Antibody

Images

 
Independent Antibodies: Western Blot: AF9 Antibody [NB100-1565] - Detection of Human AF9 on HeLa whole cell lysates using NB100-1565. AF9 was also immunoprecipitated using rabbit anti-AF9 antibodies NB100-1564, ...read more
Immunohistochemistry-Paraffin: AF9 Antibody [NB100-1565] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-AF9 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
 

Independent Antibodies

       

Order Details

AF9 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 250 and 300 of human ALL1 Fused Gene from Chromosome 9 Protein using the numbering given in entry NP_004520.1 (GeneID 4300).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MLLT3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:500 - 1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 1-4 ug/mg of lysate
  • Western Blot 1:5000-1:15000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Control
AF9 Overexpression Lysate
Publications
Read Publications using
NB100-1565 in the following applications:

  • IP
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for AF9 Antibody

  • AF-9
  • AF9myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog);translocated to, 3
  • ALL1-fused gene from chromosome 9 protein
  • myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog); translocatedto, 3
  • myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila);translocated to, 3
  • Myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein
  • protein AF-9
  • YEATS domain-containing protein 3
  • YEATS3FLJ2035

Background

The human AF9 gene is one of the most common fusion partner genes with the MLL (mixed lineage leukemia) gene resulting in AF9/MLL translocations and leukemogenesis. AF9 is highly homologous to the gene product of ENL, another common MLL fusion partner gene. The function of AF9 is not known but through studies of ENL it is proposed to be a nuclear transactivating factor. AF9 is also known as ALL1 Fused Gene from Chromosome 9 Protein, MLLT3, Myeloid/Lymphoid or Mixed-Lineage Leukemia Translocated 3, YEATS3, and FLJ2035.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

H00004298-M01
Species: Hu, Mu
Applications: ELISA, ICC/IF, IHC, IHC-P, S-ELISA, WB
NBP1-28728
Species: Hu
Applications: IHC, IHC-P, IP (-), WB
NBP1-33736
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, WB
NBP1-52105
Species: Hu, Rt
Applications: Flow, IHC, IHC-P, PEP-ELISA, WB
NBP1-84254
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-13754
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NB100-40845
Species: Hu, Mu
Applications: CHIP-SEQ, ChIP, IHC, IHC-P, IP, KD, WB
NBP1-90222
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-82991
Species: Hu
Applications: IHC, IHC-P, WB
NBP1-89222
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
AF7829
Species: Hu
Applications: ICC, IHC, WB
NBP1-30141
Species: Ce, Dr, Hu, In, Ma, Mu, Po, Rt, Ye
Applications: ChIP, ELISA, ICC/IF, IHC, IHC-P, IP, WB
AF5129
Species: Hu
Applications: WB
NB100-74357
Species: Hu, Mu, Rt
Applications: ICC/IF, WB
NBP1-89105
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
H00027125-M01
Species: Hu
Applications: ELISA, IHC, IHC-P, S-ELISA, WB
AF5414
Species: Hu
Applications: Simple Western, WB
NBP2-67811
Species: Hu, Mu
Applications: ChIP, ICC/IF, IHC, IHC-P, IP, Simple Western, WB

Publications for AF9 Antibody (NB100-1565)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IP, WB.


Filter By Application
IP
(1)
WB
(1)
All Applications
Filter By Species
Human
(2)
All Species

Reviews for AF9 Antibody (NB100-1565) (0)

There are no reviews for AF9 Antibody (NB100-1565). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for AF9 Antibody (NB100-1565) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional AF9 Products

Bioinformatics Tool for AF9 Antibody (NB100-1565)

Discover related pathways, diseases and genes to AF9 Antibody (NB100-1565). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for AF9 Antibody (NB100-1565)

Discover more about diseases related to AF9 Antibody (NB100-1565).
 

Pathways for AF9 Antibody (NB100-1565)

View related products by pathway.

PTMs for AF9 Antibody (NB100-1565)

Learn more about PTMs related to AF9 Antibody (NB100-1565).
 

Research Areas for AF9 Antibody (NB100-1565)

Find related products by research area.

Blogs on AF9

There are no specific blogs for AF9, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our AF9 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol MLLT3
Entrez
Uniprot