Adiponutrin/PNPLA3 Antibody [Unconjugated]

Western blot shows lysates of BG01V human embryonic stem cells, mouse liver tissue, human brain tissue, and mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Adiponutrin/PNPLA3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5179) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Adiponutrin/PNPLA3 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Product Discontinued

Adiponutrin/PNPLA3 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse Adiponutrin/PNPLA3
  Arg160-Arg327
  Accession # Q91WW7
• Specificity: Detects human and mouse Adiponutrin/PNPLA3 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: PNPLA3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Adiponutrin/PNPLA3 Antibody [Unconjugated]

• Acylglycerol O-acyltransferase
• Adiponutrin
• ADPN
• ADPNdJ796I17.1
• C22orf20
• Calcium-independent phospholipase A2-epsilon
• chromosome 22 open reading frame 20
• EC 2.3.1.-
• EC 2.7.7.56
• EC 3.1.1.3
• EC 4.2.3.4
• FLJ22012
• iPLA(2)epsilon
• iPLA2-epsilon
• patatin-like phospholipase domain containing 3
• patatin-like phospholipase domain-containing protein 3
• PNPLA3

Background

Mouse adiponutrin (also patatin-like phospholipase domain-containing protein 3) is a 45-48 kDa member of the adiponutrin family of phospholipase A2 enzymes. It is expressed in white and brown adipocytes, hepatocytes and brain. Adiponutrin is reduced in amount during fasting, and appears to act as an acute sensor of the body energy stores. Based on other family members, mouse adiponutrin is a type II transmembrane protein that is 413 amino acids (aa) in length. It contains one patatin domain (aa 10-179) that is associated with enzyme activity. There appear to be three splice variants. One shows a 69 aa substitution for aa 384-413, a second shows a deletion of aa 385-413, while a third shows a three aa substitution for aa 384-413. Over aa 160-327, mouse adiponutrin shares 84% and 62% aa identity with rat and human adiponutrin, respectively; and over aa 161-229, mouse adiponutrin shares 90% aa identity with human adiponutrin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PNPLA3
• Uniprot:
  • Q91WW7()