Recombinant Mouse Adiponectin/Acrp30 Protein

SDS-Page: Recombinant Mouse Adiponectin/Acrp30 Protein [NBC1-18447] - Adiponectin, 16 kDa (138 aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

• Reactivity: Mu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Mouse Adiponectin/Acrp30 Protein Summary

• Description: An un-tagged recombinant protein corresponding to the amino acids 111-247 of Mouse Adiponectin/Acrp30
  
  

  Source: E.coli

  Amino Acid Sequence: MAYMYRSAFS VGLETRVTVP NVPIRFTKIF YNQQNHYDGS TGKFYCNIPG LYYFSYHITV YMKDVKVSLF KKDKAVLFTY DQYQEKNVDQ ASGSVLLHLE VGDQVWLQVY GDGDHNGLYA DNVNDSTFTG FLLYHDTN

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: ADIPOQ
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 16 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 7.5) 50 mM NaCl, 5mM DTT, 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Mouse Adiponectin/Acrp30 Protein

• ACDC
• Acrp30
• ACRP30ADPN
• adipocyte, C1Q and collagen domain containing
• Adiponectin
• adiponectin, C1Q and collagen domain containing
• AdipoQ
• ADIPQTL1
• ApM1
• apM-1
• APM1APM-1
• C1q and collagen domain-containing protein
• GBP28
• GBP28apM1
• Gelatin-binding protein

Background

Adiponectin/Acrp30(247amino acids) is adipocyte complement-related protein of 30kDa and exclusively expressed in differentiated adipocytes. Adiponectin(Acrp30) is a member of the complement factor C1q family and consists of signal sequence, Non-homologous sequence, collagen domain and globular domain(gAcrp30). Adiponectin(Acrp30) expression is reduced in a variety of obese and insulin-resistant states in human, monkeys and mice. Injection of Acrp30(247aa) or gAcrp30(globular domain) lowers serum glucose and free fatty acid level in mice. The globular domain of adiponectin/acrp30(amino acid residues, 111-247, gAcrp30) was overexpressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: ADIPOQ