Adiponectin/Acrp30 Overexpression Lysate (Native)

Images

 
Western Blot: Adiponectin Overexpression Lysate (Adult Normal) [NBL1-07342] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Adiponectin.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Adiponectin/Acrp30 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215161 and based on accession number NM_004797. The protein contains a C-terminal DDK tag.
Epitope
C-terminal Myc/DDK
Specificity
Homo sapiens adiponectin, C1Q and collagen domain containing (ADIPOQ), mRNA.
Gene
ADIPOQ

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
24.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Adiponectin/Acrp30

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Adiponectin/Acrp30 Overexpression Lysate (Native)

  • ACDC
  • Acrp30
  • ACRP30ADPN
  • adipocyte, C1Q and collagen domain containing
  • Adiponectin
  • adiponectin, C1Q and collagen domain containing
  • AdipoQ
  • ADIPQTL1
  • ApM1
  • apM-1
  • APM1APM-1
  • C1q and collagen domain-containing protein
  • GBP28
  • GBP28apM1
  • Gelatin-binding protein

Background

This gene is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
NBP2-22106
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP1-28640
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
AF5739
Species: Hu, Mu, Rt
Applications: WB
NBP1-28641
Species: Hu, Mu, Rt, Mk
Applications: WB, IHC, IHC-P
NB100-594
Species: Hu, Mu, Ca
Applications: WB, IHC-P
NBP1-19773
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-22127
Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-P
AF2850
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, ICC
NBP1-87487
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
MAB8200
Species: Hu, Mu
Applications: IHC
NB300-537
Species: Hu, Mu, Rt, Bv, Rb
Applications: WB, ChIP, Flow, GS, ICC/IF, IHC, IHC-P, IP
NBP2-34294
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC-P
NBL1-07342
Species: Hu
Applications: WB

Publications for Adiponectin/Acrp30 Lysate (NBL1-07342) (0)

There are no publications for Adiponectin/Acrp30 Lysate (NBL1-07342).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Adiponectin/Acrp30 Lysate (NBL1-07342) (0)

There are no reviews for Adiponectin/Acrp30 Lysate (NBL1-07342). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Adiponectin/Acrp30 Lysate (NBL1-07342) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Adiponectin/Acrp30 Products

Bioinformatics Tool for Adiponectin/Acrp30 Lysate (NBL1-07342)

Discover related pathways, diseases and genes to Adiponectin/Acrp30 Lysate (NBL1-07342). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Adiponectin/Acrp30 Lysate (NBL1-07342)

Discover more about diseases related to Adiponectin/Acrp30 Lysate (NBL1-07342).
 

Pathways for Adiponectin/Acrp30 Lysate (NBL1-07342)

View related products by pathway.

PTMs for Adiponectin/Acrp30 Lysate (NBL1-07342)

Learn more about PTMs related to Adiponectin/Acrp30 Lysate (NBL1-07342).
 

Research Areas for Adiponectin/Acrp30 Lysate (NBL1-07342)

Find related products by research area.

Blogs on Adiponectin/Acrp30

There are no specific blogs for Adiponectin/Acrp30, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Adiponectin/Acrp30 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol ADIPOQ
Entrez